MolPharm xPharm- The Comprehensive Pharmacology Reference

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by CHAMBERS, J. W.
Right arrow Articles by BASS, A. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by CHAMBERS, J. W.
Right arrow Articles by BASS, A. D.

Molecular Pharmacology, Vol 1, 66-76, Copyright © 1965 by the American Society for Pharmacology and Experimental Therapeutics

Effect of Hydrocortisone and Insulin on Uptake of agr-Aminoisobutyric Acid by Isolated Perfused Rat Liver

JOHN W. CHAMBERS 1, RALPH H. GEORG 1, and ALLAN D. BASS 1

1 Department of Pharmacology, Vanderbilt University, School of Medicine, Nashville, Tennessee

In this study both hydrocortisone and insulin were shown to increase the uptake of a nonmetabolizable amino acid, agr-aminoisobutyric acid (AIB), by the isolated perfused rat liver. DNA-dependent RNA synthesis was inhibited by actinomycin D to determine whether the effects of these hormones on transport were independent of their actions on the transcription of genetic information. Actinomycin D inhibited enzyme induction by hydrocortisone approximately 90% without affecting the increase in AIB transport. However, approximately half of the insulin effect on AIB transport was blocked by actinomycin D. The effect of hydrocortisone on the uptake of AIB was completely inhibited by phenoxybenzamine (PBZ), an adrenergic blocking agent. The action of insulin on AIB uptake was not affected by PBZ. Hydrocortisone and insulin together exerted an additive effect on the hepatic uptake of AIB. Both hormones act directly (but apparently at different sites) to increase the AIB uptake by the liver. Most of the steroid action and approximately half of the insulin action appears to be independent of any effect these hormones have on DNA-dependent RNA synthesis in the liver.

Note:
ACKNOWLEDGMENTS This work was supported, in part, by U.S. Public Health Grant CA-02020 and, in part, by U.S. Public Health Grant 2-RO1-AM-05474. J. W. C., while a predoctoral trainee, was supported, in part, by U.S. Public Health Training Grant 2-T1-GM-58 and, in part, by U.S. Public Health Predoctoral Fellowship GFM-16,435.

Submitted on April 1, 1965




This article has been cited by other articles:


Home page
 ScienceHome page
E. L. Krawitt, E. F. Baril, J. E. Becker, and V. R. Potter
Amino Acid Transport in Hepatoma Cell Cultures during Tyrosine Aminotransferase Induction
Science, July 17, 1970; 169(3942): 294 - 296.
[Abstract] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1965 by the American Society for Pharmacology and Experimental Therapeutics