Molecular Pharmacology, Vol 11, 520-527, Copyright © 1975 by the American Society for Pharmacology and Experimental Therapeutics

Comparative Metabolic Effects Of Chloramphenicol Analogues

¹ Departments of Medicine and Biochemistry, University of Miami School of Medicine, and the Howard Hughes Medical Institute, Miami, Florida 33152

Two groups of chloramphenicol analogues have been examined with respect to their effect on mitochondrial and Escherichia coli protein synthesis and on DNA synthesis in cultured human lymphoblastoid cells; one group of analogues with substitutions in the para position of the ring and another in which the substitutions were in the propanediol and dichloroacetamide moieties of the molecule. In general, substitutions in the propanediol or dichloroacetamide portion of the molecule had profound effects upon its capacity to inhibit both mitochondrial and E. coli protein synthesis. Although the patterns of inhibition of both mitochondrial and E. coli protein synthesis were similar with most of the analogues, there were several exceptions, suggesting some basic differences in these two processes. Substitutions in the para position of the molecule did not influence in any recognizable pattern its effect on either protein or DNA synthesis.

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ACKNOWLEDGMENT The authors wish to acknowledge the expert technical assistance of Miss Olga Carvajalino, who assisted in many phases of this work.