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Molecular Pharmacology, Vol 13, 652-661, Copyright © 1977 by the American Society for Pharmacology and Experimental Therapeutics

Active Dimers of Dopamine beta-Hydroxylase in Human Plasma

ROBERT C. ROSENBERG 1 and WALTER LOVENBERG 1

1 Section on Biochemical Pharmacology, Hypertension-Endocrine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20014

Normal human plasma and serum have been shown to contain two active species of dopamine beta-hydroxylase (EC 1.14.17.1) by gradient ultracentrifugation and gel filtration. About 75-80% of the dopamine beta-hydroxylase activity in plasma sediments almost exactly like bovine dopamine beta-hydroxylase, and has a molecular weight of 289,000. The remainder of the dopamine beta-hydroxylase activity in plasma or serum (20-25%) has a molecular weight of 147,000, or one-half that of the principal species. No interconversion is observed between these two species at pH 7.4, ionic strength 0.15. Thus a significant amount of dopamine beta-hydroxylase activity in plasma is in the form of an active dimer of the basic subunit of this enzyme.

Submitted on September 15, 1976
Accepted on February 1, 1977






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