MolPharm xPharm- The Comprehensive Pharmacology Reference

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by PRÉMONT, J.
Right arrow Articles by BOCKAERT, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by PRÉMONT, J.
Right arrow Articles by BOCKAERT, J.

Molecular Pharmacology, Vol 13, 662-670, Copyright © 1977 by the American Society for Pharmacology and Experimental Therapeutics

Adenosine-Sensitive Adenylate Cyclase in Rat Striatal Homogenates and Its Relationship to Dopamine- and Ca2+-Sensitive Adenylate Cyclases

JOËL PRÉMONT 1, MICHÈLE PEREZ 1, and JOËL BOCKAERT 1

1 Laboratoires de Physiologie Cellulaire, 75231 Paris Cedex 05, France

Rat striatal homogenates were found to contain adenosine-sensitive adenylate cyclase, which could be fully stimulated by endogenous adenosine under standard adenylate cyclase assay conditions. Adenosine present in the adenylate cyclase assay had two origins, the homogenate and ATP hydrolysis during incubation. Theophylline, caffeine, and isobutylmethylxanthine inhibited the adenosine-sensitive adenylate cyclase. At low concentrations, theophylline inhibition was competitive (KI = 20 µM). The adenosine present in the assay could be removed by using adenosine deaminase. It was then possible to stimulate the adenosine-sensitive adenylate cyclase by adenosine analogues resistant to adenosine deaminase treatment, such as 2-chloroadenosine, N6-phenylisopropyladenosine, and 5'-deoxyadenosine, which was a partial agonist. Adenine, inosine, 5'-AMP, and ATP were neither agonists non antagonists. In addition to adenosine-sensitive adenylate cyclase, rat striatal homogenates contain Ca2+- and dopamine-sensitive adenylate cyclases. Each of these three adenylate cyclase systems was found to be independent of the others.

Note:
ACKNOWLEDGMENTS We are indebted to Drs. J. P. Tassin, A. M. Thierry, and J. Glowinski for their helpful criticisms of this work, and to Miss M. Dreyfus and Mrs. M. Guérin for their help in the preparation of the manuscript.

Submitted on December 23, 1976
Accepted on February 28, 1977




This article has been cited by other articles:


Home page
 Endocr. Rev.Home page
R. G. Smith, L. Betancourt, and Y. Sun
Molecular Endocrinology and Physiology of the Aging Central Nervous System
Endocr. Rev., April 1, 2005; 26(2): 203 - 250.
[Abstract] [Full Text] [PDF]

Home page
 NeurologyHome page
B. B. Fredholm and P. Svenningsson
Adenosine-dopamine interactions: Development of a concept and some comments on therapeutic possibilities
Neurology, December 9, 2003; 61(90116): S5 - 9.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
D. Herve, C. Le Moine, J.-C. Corvol, L. Belluscio, C. Ledent, A. A. Fienberg, M. Jaber, J.-M. Studler, and J.-A. Girault
G{alpha}olf Levels Are Regulated by Receptor Usage and Control Dopamine and Adenosine Action in the Striatum
J. Neurosci., June 15, 2001; 21(12): 4390 - 4399.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1977 by the American Society for Pharmacology and Experimental Therapeutics