MolPharm xPharm- The Comprehensive Pharmacology Reference

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by CANTRELL, C. E.
Right arrow Articles by PRUITT, K. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by CANTRELL, C. E.
Right arrow Articles by PRUITT, K. M.

Molecular Pharmacology, Vol 15, 322-330, Copyright © 1979 by the American Society for Pharmacology and Experimental Therapeutics

Efficiency of Photolytic Binding of Ethidium Monoazide to Nucleic Acids and Synthetic Polynucleotides

CHARLES E. CANTRELL 1, K. LEMONE YIELDING 1, and KENNETH M. PRUITT 1

1 Laboratory of Molecular Biology, University of Alabama in Birmingham, University Station Birmingham, Alabama 35294

Radiolabeled 3-amino-8-azido-5-ethyl-[14C]-6-phenyl phenanthridine (ethidium monoazide) was photolyzed in situ in the presence of calf thymus DNA, RNA, poly A, calf thymus histones and nucleoid structures prepared from lymphocytes. Non-linear least squares analysis of the binding data enabled us to predict the amount of drug adduct formed at infinite polymer concentration. Photolytic efficiency could the be calculated since the input drug concentration was constant and therefore represented the non-covalent complex expected at infinite polymer concentration. The efficiency of photolysis for native, heat-denatured and formaldehyde-denatured DNA was 0.23, 0.26 and 0.45, respectively, and for RNA it was found to be 0.29. The value for poly A was 0.42. The intensity of light was not a limiting factor under the conditions of these experiments.

Submitted on June 2, 1978
Accepted on October 18, 1978




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. Wu, W. Chen, E. Murphy, S. Gabel, K. B. Tomer, J. Foley, C. Steenbergen, J. R. Falck, C. R. Moomaw, and D. C. Zeldin
Molecular Cloning, Expression, and Functional Significance of a Cytochrome P450 Highly Expressed in Rat Heart Myocytes
J. Biol. Chem., May 9, 1997; 272(19): 12551 - 12559.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Zabner, A. J. Fasbender, T. Moninger, K. A. Poellinger, and M. J. Welsh
Cellular and Molecular Barriers to Gene Transfer by a Cationic Lipid
J. Biol. Chem., August 11, 1995; 270(32): 18997 - 19007.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1979 by the American Society for Pharmacology and Experimental Therapeutics