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Molecular Pharmacology, Vol 16, 196-201, Copyright © 1979 by the American Society for Pharmacology and Experimental Therapeutics

Gonadotropin Releasing Hormone Stimulation of Cultured Pituitary Cells Requires Calcium

JAN MARIAN 1 and P. MICHAEL CONN 1

1 Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27710

The requirement for Ca2+ during luteinizing hormone (LH) release stimulated by gonadotropin-releasing hormone (GnRH) was studied in dispersed cultured rat pituicytes. With maximal GnRH stimulation, LH release required 1 mM Ca2+ for optimal stimulation, although basal production was not calcium dependent. Stimulated release was inhibited when cells were incubated in media lacking Ca2+, and by LaCl3 (a potent inhibitor of Ca2+ action) which inhibited GnRH-induced LH release in the presence of Ca2+. The requirement for Ca2+ is specific, since Mg2+ is not as effective, even at elevated concentrations. Two agents that block Ca2+ movement into and within cells, Ruthenium Red and D-600 (methoxy-verapamil), also blocked the release of LH from stimulated pituicytes. The present results demonstrate a specific dependence on calcium for GnRH stimulation of LH release from cultured gonadotrophs. Our observations satisfy some of the criteria require for a postulated role of Ca2+ in stimulus-secretion coupling in this system.

Submitted on December 20, 1978
Accepted on February 20, 1979




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Copyright © 1979 by the American Society for Pharmacology and Experimental Therapeutics