Molecular Pharmacology, Vol 16, 473-481, Copyright © 1979 by the American Society for Pharmacology and Experimental Therapeutics

Response of Adenosine Cyclic 3',5'-Monophosphate Level in Rabbit Neutrophils to the Chemotactic Peptide Formyl-Methionyl-Leucyl-Phenylalanine

¹ Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut 06032

Addition of the synthetic chemotactic peptide, fMet-Leu-Phe, to rabbit neutrophils elevates the basal level of cyclic 3',5'-AMP. Stimulation of the cyclic AMP level reaches a maximum at 1 min (60% increase over basal value at 5 nM fMet-Leu-Phe) and subsequently the cyclic AMP level returns to the basal value within 5 min. In the absence of extracellular Ca²⁺, the cyclic AMP level increases minimally (<20%) upon exposure to fMet-Leu-Phe. The effect of fMet-Leu-Phe is detectable at 0.5 nM and increases at higher concentrations. Inhibition of the cyclic AMP-dependent phosphodiesterase activity by pretreatment with 1-methyl-3-isobutylxanthine enhances both the basal and stimulated cyclic AMP levels.

Note:
ACKNOWLEDGMENTS We thank Dr. P. H. Naccache for many timely discussions and Dr. M. C. Wacholtz for helpful information in the course of this work. We also thank Dr. R. J. Freer for supplying the chemotactic peptide.

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