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Molecular Pharmacology, Vol 18, 148-150, Copyright © 1980 by the American Society for Pharmacology and Experimental Therapeutics

The Incorporation of Trifluorothymidine into Calf Thymus DNA in a Cell-Free System Does Not Lead to Chain Termination

AWNI M. SARRIF 1, HIROSHI TONE 2, PETER V. DANENBERG 3, and CHARLES HEIDELBERGER 3

1 Department of Pharmacology and Comprehensive Cancer Center, University of Alabama in Birmingham Medical Center, Birmingham, Alabama 35294
2 Central Research Laboratories, Sanraku-Ocean Company Ltd., Fujisawa, 251 Japan.
3 Department of Biochemistry and Comprehensive Cancer Center, University of Southern California Medical Center, Los Angeles California 90033

In this report we present evidence that trifluorothymidine-5'-triphosphate (F3dTTP) once incorporated into activated calf thymus DNA by a partially purified HeLa cell cytoplasmic DNA-dependent DNA polymerase agr does not lead to chain termination. Less than 1% of the [3H]F3dTTP incorporated in the cell-free system was recovered at the 3'-termini. In addition, F3dTTP-containing DNA was capable of incorporating [3H]dTTP, which led to the complete disappearance of radioactive trifluorothymidine from the 3'-termini.

Note:
ACKNOWLEDGMENTS The authors acknowledge Ms. Virginia Garcia for her technical assistance and Mrs. Sharon Garrison for the preparation of the manuscript.

Submitted on October 15, 1980
Accepted on February 5, 1980






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