MolPharm xPharm- The Comprehensive Pharmacology Reference

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by FIRNAU, G.
Right arrow Articles by GARNETT, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by FIRNAU, G.
Right arrow Articles by GARNETT, S.

Molecular Pharmacology, Vol 19, 130-133, Copyright © 1981 by the American Society for Pharmacology and Experimental Therapeutics

Phenol Ionization in Dopa Determines the Site of Methylation by Catechol-O-Methyltransferase

GUNTER FIRNAU 1, SUDESH SOOD 1, RICHARD PANTEL 1, and STEPHEN GARNETT 1

1 McMaster University Medical Centre, Department of Nuclear Medicine, Hamilton, Ontario, Canada L8S 4J9

Catechol-O-methyltransferase (COMT, EC 2.1.1.6) with S-adenosylmethionine as the methyl donor catalyzes preferentially p-O-methylation when 5-fluorodopa is the substrate. The rate of this reaction is characterized by a Km of 0.4 ± 0.2 mmole/liter and a Vmax of 27 ± 11 mmoles/mg of COMT per minute at pH 7.9. We conclude that these results are a consequence of fluorine-induced ionization at the p-hydroxyl group.

Note:
ACKNOWLEDGMENT We are grateful to Dr. C. R. Creveling, Natonal Institutes of Health, Bethesda, Md., who provided 3-(4-hydroxy-3-methoxyphenyl)-L-alanine.

Submitted on December 7, 1979
Accepted on September 3, 1980






Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1981 by the American Society for Pharmacology and Experimental Therapeutics