meta- and para-isothiocyanato-t-butylbicycloorthobenzoate: irreversible ligands of the gamma-aminobutyric acid-regulated chloride ionophore

AH Lewin, BR de Costa, KC Rice and P Skolnick

Laboratory of Neuroscience, National Institute of Diabetes, Digestive, and Kidney Diseases, Bethesda, MD 20892.

The meta- and para-isothiocyanato derivatives of t- butylbicycloorthobenzoate (TBOB) were synthesized by catalytic reduction of the corresponding nitro compounds, followed by treatment with thiophosgene. p-NCS-TBOB (2) inhibited the binding of both [3H]TBOB and [35S]t-butylbicyclophosphorothionate (TBPS) with potencies (IC50 of 61 and 23 nM, respectively) similar to the parent compound. In contrast, the meta derivative (m-NCS-TBOB, 1) was more than 1 order of magnitude less potent (IC50 of 1588 and 149 nM, respectively). The IC50 values for both 1 and 2 were strongly dependent on the tissue concentration, in a manner characteristic of irreversible inhibitors. Moreover, preincubation of tissue with these compounds, followed by extensive washing, resulted in a concentration-dependent reduction in the number of [35S]TBPS binding sites and in the apparent affinity of this radioligand. Similar effects were not observed in tissues treated in identical fashion with either TBOB or picrotoxin. Preincubation with p-NCS-TBOB at concentrations that significantly inhibit [35S]TBPS or [3H]TBOB binding did not affect radioligand binding to either benzodiazepine or gamma-aminobutyric acid receptors. These findings suggest that m- and p-NCS-TBOB bind irreversibly to sites labeled by cage convulsants such as TBOB and TBPS, which are on or near GABA-gated chloride channels. p-NCS-TBOB should prove useful in determining the molecular characteristics of the benzodiazepine receptor-coupled GABA- gated chloride ionophore.

Volume 35, Issue 2, pp. 189-194, 02/01/1989
Copyright © 1989 by American Society for Pharmacology and Experimental Therapeutics