![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
P Palade, C Dettbarn, B Alderson and P Volpe
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston 77550.
Various known Ca2+ channel blockers and intracellular Ca2+ antagonists have been tested for effects of inositol-1,4,5-trisphosphate (IP3)- induced Ca2+ release from isolated canine brain microsomes. In agreement with previous reports, heparin, p-chloromercuribenzoic acid, W-7, cinnarizine, flunarizine, certain local anesthetics, La3+, and Ca2+ inhibit the release of Ca2+ induced by addition of IP3. In addition, we report here pronounced inhibition of IP3-induced Ca2+ release by low levels of Cd2+, by relatively high concentrations of TMB- 8, and by phytic acid. In contrast, a number of blockers of other Ca2+ channels (nifedipine, verapamil, dantrolene, dithiothreitol, and ruthenium red) have relatively little or no effect on IP3-induced Ca2+ release from brain microsomes. The relative ineffectiveness of substances that inhibit Ca2+- or caffeine-induced Ca2+ release from skeletal muscle sarcoplasmic reticulum suggests that release of Ca2+ from caffeine- and IP3-sensitive neuronal Ca2+ stores is likely to be mediated by different channels. Further evidence that different channels are involved is presented by way of demonstration of the lack of Ca2+-induced Ca2+ release from these brain microsomes and the lack of effect on sarcoplasmic reticulum caffeine-induced Ca2+ release of certain inhibitors of IP3-induced Ca2+ release used here. Among IP3- induced Ca2+ release blockers, La3+ appeared to be exceptional in its ability to stimulate microsomal Ca2+ uptake sufficiently to attenuate release of Ca2+ induced by IP3. Most blockers of IP3-induced Ca2+ release appear not to function by way of inhibiting K+ counter-ion movements (valinomycin does not reverse the inhibition) but rather by way of direct interaction with the IP3 receptor or the Ca2+ channel that mediates the IP3-induced Ca2+ release. Inhibition of [3H]IP3 binding to the microsomes by phytic acid, heparin, pyrophosphate, p- chloromercuribenzoic acid, and Ca2+ could be demonstrated but not by the other substances tested.
This article has been cited by other articles:
|
|
 |
|
  A. Reynolds, A. Parris, L. A. Evans, S. Lindqvist, P. Sharp, M. Lewis, R. Tighe, and M. R. Williams Dynamic and differential regulation of NKCC1 by calcium and cAMP in the native human colonic epithelium J. Physiol., July 15, 2007; 582(2): 507 - 524. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Kossintseva, S. Wong, E. Johnstone, L. Guilbert, D. M. Olson, and B. F. Mitchell Proinflammatory cytokines inhibit human placental 11{beta}-hydroxysteroid dehydrogenase type 2 activity through Ca2+ and cAMP pathways Am J Physiol Endocrinol Metab, February 1, 2006; 290(2): E282 - E288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Verkhratsky Physiology and Pathophysiology of the Calcium Store in the Endoplasmic Reticulum of Neurons Physiol Rev, January 1, 2005; 85(1): 201 - 279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Fellner and L. A. Parker Endothelin B receptor Ca2+ signaling in shark vascular smooth muscle: participation of inositol trisphosphate and ryanodine receptors J. Exp. Biol., September 1, 2004; 207(19): 3411 - 3417. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. B. Lomax, C. Camello, F. Van Coppenolle, O. H. Petersen, and A. V. Tepikin Basal and Physiological Ca2+ Leak from the Endoplasmic Reticulum of Pancreatic Acinar Cells. SECOND MESSENGER-ACTIVATED CHANNELS AND TRANSLOCONS J. Biol. Chem., July 12, 2002; 277(29): 26479 - 26485. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Fellner and L. Parker A Ca2+-sensing receptor modulates shark rectal gland function J. Exp. Biol., July 1, 2002; 205(13): 1889 - 1897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Salomonsson and W. J. Arendshorst Norepinephrine-induced calcium signaling pathways in afferent arterioles of genetically hypertensive rats Am J Physiol Renal Physiol, August 1, 2001; 281(2): F264 - F272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. Poirier, M. Poitras, K. Laflamme, and G. Guillemette Thiol-Reactive Agents Biphasically Regulate Inositol 1,4,5-Trisphosphate Binding and Ca2+ Release Activities in Bovine Adrenal Cortex Microsomes Endocrinology, June 1, 2001; 142(6): 2614 - 2621. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Hauser, Z. Fekete, J. M. Adams, M. Garced, D. H. Livingston, and E. A. Deitch PAF-mediated Ca2+ influx in human neutrophils occurs via store-operated mechanisms J. Leukoc. Biol., January 1, 2001; 69(1): 63 - 68. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. E. PURDY and W. J. ARENDSHORST Iloprost Inhibits Inositol-1,4,5-Trisphosphate-Mediated Calcium Mobilization Stimulated by Angiotensin II in Cultured Preglomerular Vascular Smooth Muscle Cells J. Am. Soc. Nephrol., January 1, 2001; 12(1): 19 - 28. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. Koulen and B. E. Ehrlich Reversible Block of the Calcium Release Channel/Ryanodine Receptor by Protamine, a Heparin Antidote Mol. Biol. Cell, July 1, 2000; 11(7): 2213 - 2219. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. K. Combs, D. E. Johnson, S. B. Cannady, T. M. Lehman, and G. E. Landreth Identification of Microglial Signal Transduction Pathways Mediating a Neurotoxic Response to Amyloidogenic Fragments of beta -Amyloid and Prion Proteins J. Neurosci., February 1, 1999; 19(3): 928 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Horgan and P. F. Copenhaver G Protein-Mediated Inhibition of Neuronal Migration Requires Calcium Influx J. Neurosci., June 1, 1998; 18(11): 4189 - 4200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Patel, S. A. Morris, C. E. Adkins, G. O'Beirne, and C. W. Taylor Ca2+-independent inhibition of inositol trisphosphate receptors by calmodulin: Redistribution of calmodulin as a possible means of regulating Ca2+ mobilization PNAS, October 14, 1997; 94(21): 11627 - 11632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Bandtlow, M. Schmidt, T. Hassinger, M. Schwab, and S. Kater Role of intracellular calcium in NI-35-evoked collapse of neuronal growth cones Science, January 1, 1993; 259(5091): 80 - 83. [Abstract] [PDF]
|
|
|
|
|
|
A Tse and B Hille GnRH-induced Ca2+ oscillations and rhythmic hyperpolarizations of pituitary gonadotropes Science, January 24, 1992; 255(5043): 462 - 464. [Abstract] [PDF]
|
|
|
|
|
|
A. Harootunian, J. Kao, S Paranjape, and R. Tsien Generation of calcium oscillations in fibroblasts by positive feedback between calcium and IP3 Science, January 4, 1991; 251(4989): 75 - 78. [Abstract] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
