MolPharm xPharm- The Comprehensive Pharmacology Reference

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dogra, S.
Right arrow Articles by Oesch, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dogra, S.
Right arrow Articles by Oesch, F.

Stable expression of rat cytochrome P-450IA1 cDNA in V79 Chinese hamster cells and their use in mutagenicity testing

S Dogra, J Doehmer, H Glatt, H Molders, P Siegert, T Friedberg, A Seidel and F Oesch

Institut fur Toxikologie, Johannes Gutenberg-Universitat Mainz, Federal Republic of Germany.

V79 Chinese hamster cells genetically engineered to express cytochrome P-450IA1 are reported. A full length cDNA encoding rat cytochrome P- 450IA1 was obtained from a cDNA library prepared from rat liver mRNA. The cDNA was recombined with the SV40 early promoter and expressed in V79 cells. Three V79-derived P-450IA1-expressing cell lines (XEM1, XEM2, and XEM3) were established. The presence of the rat cytochrome P- 450IA1 cDNA in these hamster cells was confirmed by Southern blotting. The transcription of the cDNA into mRNA and translation into the desired cytochrome P-450 protein was detected by Northern and Western blotting. The enzymatic activity was determined by the cytochrome P- 450IA1-dependent oxidation of benzo[a]pyrene and 7-ethoxycoumarin. After exposure to benzo[a]pyrene, the mutant frequency increased in XEM1 and XEM2 cells and was higher than in V79 cells in the presence of an exogenous activating system. The mutant frequency was even more increased when XEM1 and XEM2 cells were exposed to the proximate mutagen (trans)-7,8-dihydroxy-7,8-dihydro-benzo[a]pyrene.

Volume 37, Issue 5, pp. 608-613, 05/01/1990
Copyright © 1990 by American Society for Pharmacology and Experimental Therapeutics




This article has been cited by other articles:


Home page
 MutagenesisHome page
H. Helleberg, H. Xu, L. Ehrenberg, K. Hemminki, U. Rannug, and M. Tornqvist
Studies of dose distribution, premutagenic events and mutation frequencies for benzo[a]pyrene aiming at low dose cancer risk estimation
Mutagenesis, July 1, 2001; 16(4): 333 - 337.
[Abstract] [Full Text] [PDF]

Home page
 MutagenesisHome page
K. G. Jensen, K. Wiberg, E. Klasson-Wehler, and A. Onfelt
Induction of aberrant mitosis with PCBs: particular efficiency of 2,3,3',4,4'-pentachlorobiphenyl and synergism with triphenyltin
Mutagenesis, January 1, 2000; 15(1): 9 - 15.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
W. Engst, R. Landsiedel, H. Hermersdorfer, J. Doehmer, and H. Glatt
Benzylic hydroxylation of 1-methylpyrene and 1-ethylpyrene by human and rat cytochromes P450 individually expressed in V79 Chinese hamster cells
Carcinogenesis, September 1, 1999; 20(9): 1777 - 1785.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1990 by the American Society for Pharmacology and Experimental Therapeutics