MolPharm xPharm- The Comprehensive Pharmacology Reference

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by BLUM, J. J.
Right arrow Articles by WEXLER, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by BLUM, J. J.
Right arrow Articles by WEXLER, J. P.

Molecular Pharmacology, Vol 4, 155-161, Copyright © 1968 by the American Society for Pharmacology and Experimental Therapeutics

Effect of Clofibrate on Tetrahymena

J. J. BLUM 1 and J. P. WEXLER 1

1 Department of Physiology and Pharmacology, Duke University School of Medicine, Durham, North Carolina 27706

The effects of clofibrate on the growth, glycogen content, triglyceride content, and levels of some peroxisomal enzymes of the ciliated protozoan Tetrahymena pyriformis have been studied. Clofibrate sensitivity inhibited growth and depleted the cell glycogen. The triglyceride content of Tetrahymena in Ringer's solution in the absence of an exogenous carbon source was doubled by exposure to clofibrate for 2 hr. Cells grown in the presence of clofibrate for 17 hr showed a 30% increase in isocitric dehydrogenase activity, a 30% decrease in isocitric lyase activity, and a 20% increase in catalase activity. The results are consistent with the hypothesis that clofibrate inhibits gluconeogenesis or activates glycogenolysis.

Note:
ACKNOWLEDGMENTS This work was supported by grants from the National Science Foundation (No. GB-2788) and the National Institutes of Health (No. 5-RO1-HD-01269). Dr. Blum is the recipient of a Research Career Development Award (No. K3-GM-2341). Mr. Wexler is a predoctral trainee, supported by a Public Health Service training grant (No. 5-T01-GM-00929) from the National Institutes of Health. Miss Delores Randolph and Mr. Alvernon Hayes provided excellent technical assistance. We are also grateful to Dr. B. Wittels for instruction in the techniques of lipid analysis.

Submitted on September 22, 1967






Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1968 by the American Society for Pharmacology and Experimental Therapeutics