Molecular Pharmacology, Vol 4, 379-388, Copyright © 1968 by the American Society for Pharmacology and Experimental Therapeutics

Studies on Adenosine 3',5'-Phosphate in Rabbit Cerebral Cortex

¹ Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106

Incubation of slices of rabbit cerebral cortex with histamine resulted in large accumulations of adenosine 3',5'-phosphate. Maximal levels of the nucleotide required about 15 min exposure to histamine and represented more than an 8-fold increase. Inclusion of theophylline (0.5 mM) in incubation media had little effect by itself on the accumulation of adenosine 3',5'-phosphate but increased the effect of histamine 3-fold. Norepinephrine increased the nucleotide level only about 70%, but only 2 min exposure to the agent was required to produce maximal values. This pattern of responses is in sharp contrast to that observed in cerebellar slices, but appears to be similar to that of other areas of rabbit brain. The content of adenosine 3',5'-phosphate in the cerebral cortex of rabbit heads frozen rapidly at intervals after decapitation rose about 8-fold within 90 sec after decapitation. Although phosphorylase a levels increased very rapidly after decapitation, no changes in phosphorylase a content of either cerebellar or cerebral cortex slices was detected under conditions producing large increases in adenosine 3',5'-phosphate.

Note:
ACKNOWLEDGMENTS This work was supported in part by U.S. Public Health Service Grants AM-06141, NB-05716, and GM-661. The authors also wish to acknowledge the skillful technical assistance of Mrs. Arleen Maxwell Haley, Mr. George Thorne, and Mr. Neil Joebchen.

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