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Molecular Pharmacology, Vol 4, 640-644, Copyright © 1968 by the American Society for Pharmacology and Experimental Therapeutics

Studies on Serum Cholinesterase Kinetics by Nuclear Magnetic Resonance Spectroscopy

G. KATO 1

1 Wellcome Department of Research in Anaesthesia, McGill University, Montreal, Canada

The hydrolysis of acetylcholine by horse serum cholinesterase has been measured by nuclear magnetic resonance spectroscopy. The reaction rate was estimated from the formation of free acetate, whose characteristic frequency of absorption differs from that of the acetate moiety of acetylcholine. From the decay of the acetate signal of acetylcholine and/or the formation of the sodium acetate signal in sodium phosphate buffer, it is possible to follow the rate of the reaction. The calculated Km for the substrate and the Ki for various inhibitors agree well with values obtained by others using conventional methods.

Note:
ACKNOWLEDGMENT I wish to thank Professor K. Krnjevicacute for his advice and for reading the manuscript.

Submitted on August 8, 1968






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