MolPharm xPharm- The Comprehensive Pharmacology Reference

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jaiswal, N.
Right arrow Articles by Malik, K. U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jaiswal, N.
Right arrow Articles by Malik, K. U.

Muscarinic receptor-mediated prostacyclin and cGMP synthesis in cultured vascular cells

N Jaiswal, RK Jaiswal and KU Malik

Department of Pharmacology, University of Tennessee, Memphis 38163.

The purpose of the present study was to determine the subtype of muscarinic receptor involved in the action of cholinergic stimuli on synthesis of prostacyclin, measured as immunoreactive 6-keto- prostaglandin F1 alpha (6-keto-PGF1 alpha), and cGMP in bovine aortic endothelial and rabbit vascular smooth muscle cells. Acetylcholine and arecaidine propargyl ester, a selective M2 agonist, produced a dose- dependent increase in 6-keto-PGF1 alpha output and cGMP formation in confluent endothelial cells but not in confluent vascular smooth muscle cells. McN-A-343, a selective M1 agonist, failed to alter basal 6-keto- PGF1 alpha or cGMP synthesis. Acetylcholine- and arecaidine propargyl ester-induced 6-keto-PGF1 alpha synthesis and cGMP formation in endothelial cells were attenuated by atropine, AF-DX 116 (M2 antagonist), and hexahydrosiladifenidol (M3 antagonist) but not by pirenzepine (M1 antagonist). The cyclooxygenase inhibitor indomethacin abolished 6-keto-PGF1 alpha synthesis but not the increase in cGMP formation elicited by the cholinergic stimuli. Our data suggest that the effect of cholinergic stimuli to enhance prostacyclin and cGMP synthesis is mediated via activation of M2 and M3 receptors located on endothelial cells and that the increase in cGMP production is independent of prostaglandins.

Volume 40, Issue 1, pp. 101-106, 07/01/1991
Copyright © 1991 by American Society for Pharmacology and Experimental Therapeutics




This article has been cited by other articles:


Home page
 CarcinogenesisHome page
J.-H. Jeng, Y.-J. Wang, B.-L. Chiang, P.-H. Lee, C.-P. Chan, Y.-S. Ho, T.-M. Wang, J.-J. Lee, L.-J. Hahn, and M.-C. Chang
Roles of keratinocyte inflammation in oral cancer: regulating the prostaglandin E2, interleukin-6 and TNF-{alpha} production of oral epithelial cells by areca nut extract and arecoline
Carcinogenesis, August 1, 2003; 24(8): 1301 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
X. Yao, P.-S. Leung, H.-Y. Kwan, T.-P. Wong, and M.-W. Fong
Rod-type cyclic nucleotide-gated cation channel is expressed in vascular endothelium and vascular smooth muscle cells
Cardiovasc Res, January 1, 1999; 41(1): 282 - 290.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
H. Kan, Y. Ruan, and K. U. Malik
Signal Transduction Mechanism(s) Involved in Prostacyclin Production Elicited by Acetylcholine in Coronary Endothelial Cells of Rabbit Heart
J. Pharmacol. Exp. Ther., July 1, 1997; 282(1): 113 - 122.
[Abstract] [Full Text]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1991 by the American Society for Pharmacology and Experimental Therapeutics