![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
MW Hamblin and MA Metcalf
Geriatric Research, Education, and Clinical Center, Seattle Veterans Affairs Medical Center, Washington 98108.
We describe the nucleic acid sequence encoding a human 5- hydroxytryptamine1D (5-HT1D) serotonin receptor and some of the functional characteristics of the gene product. The receptor gene was isolated by hybridization to a probe based on a canine thyroid cDNA (called RDC4) previously isolated by others and believed to encode a heretofore undetermined member of the guanine nucleotide-binding protein (G protein)-linked receptor family. The human clone we isolated, called MA6A, contains an apparently intronless open reading frame encoding a 377-amino acid polypeptide with the seven hydrophobic domains characteristic of G protein-linked receptors. The MA6A deduced amino acid sequence is 88% identical to that for RDC4 and 43% identical to that for the human 5-HT1A receptor. Expression of the human gene product in transfected cell lines results in the appearance of saturable high affinity 5-HT1D-type [3H]5-HT binding. The expressed receptor exhibits features indicative of coupling to Gi proteins, i.e., robust inhibition of forskolin-stimulated cAMP accumulation and formation of a pertussis toxin-sensitive high agonist affinity binding state. These findings may help clarify several ambiguities in the classification and action of serotonin receptor subtypes.
This article has been cited by other articles:
|
|
 |
|
  M. Raiteri Functional pharmacology in human brain. Pharmacol. Rev., June 1, 2006; 58(2): 162 - 193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Morikawa, O. J. Manzoni, J. C. Crabbe, and J. T. Williams Regulation of Central Synaptic Transmission by 5-HT1B Auto- and Heteroreceptors Mol. Pharmacol., April 13, 2001; 58(6): 1271 - 1278. [Abstract] [Full Text]
|
|
|
|
|
|
R. Brys, K. Josson, M. P. Castelli, M. Jurzak, P. Lijnen, W. Gommeren, and J. E. Leysen Reconstitution of the Human 5-HT1D Receptor-G-Protein Coupling: Evidence for Constitutive Activity and Multiple Receptor Conformations Mol. Pharmacol., June 1, 2000; 57(6): 1132 - 1141. [Abstract] [Full Text]
|
|
|
|
|
|
G. Pineyro and P. Blier Autoregulation of Serotonin Neurons: Role in Antidepressant Drug Action Pharmacol. Rev., September 1, 1999; 51(3): 533 - 591. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Bae, T. M. Cabrera-Vera, K. M. Depree, S. G. Graber, and H. E. Hamm Two Amino Acids within the alpha 4 Helix of Galpha i1 Mediate Coupling with 5-Hydroxytryptamine1B Receptors J. Biol. Chem., May 21, 1999; 274(21): 14963 - 14971. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ishida, K.-i. Hirata, T. Sakoda, S. Kawashima, H. Akita, and M. Yokoyama Identification of mRNA for 5-HT1 and 5-HT2 receptor subtypes in human coronary arteries Cardiovasc Res, January 1, 1999; 41(1): 267 - 274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. A. Cox, C. P. Tensen, R. C. Van der Schors, K. W. Li, H. van Heerikhuizen, E. Vreugdenhil, W. P. M. Geraerts, and J. F. Burke Cloning, Characterization, and Expression of a G-Protein-Coupled Receptor from Lymnaea stagnalis and Identification of a Leucokinin-Like Peptide, PSFHSWSamide, as Its Endogenous Ligand J. Neurosci., February 15, 1997; 17(4): 1197 - 1205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Arora, A. Sakai, and K. J. Catt Effects of Second Intracellular Loop Mutations on Signal Transduction and Internalization of the Gonadotropin-releasing Hormone Receptor J. Biol. Chem., September 29, 1995; 270(39): 22820 - 22826. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
