![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
DM Lovinger and G White
Section of Electrophysiology, National Institute on Alcohol Abuse and Alcoholism, Rockville, Maryland 20852.
Recent studies indicate that ethanol (EtOH) potentiates ion current through the channel associated with the 5-hydroxytryptamine3 (5-HT3)- type serotonin receptor. The present study was designed to determine 1) whether such potentiation occurs in adult mammalian neurons expressing 5-HT3 receptors; 2) whether potentiation is selective for the 5-HT3 receptor, relative to other ligand-gated ion channels; and 3) possible mechanisms by which EtOH potentiates this response. EtOH potentiated 5- HT3 receptor-mediated ion current in freshly isolated nodose ganglion neurons at concentrations similar to those previously reported to be effective in neuroblastoma cells (25-100 mM). Current was blocked by the selective 5-HT3 antagonist ICS 205-930 even in the presence of EtOH, and current activated by a 5-HT3 agonist (2-methyl-5-HT) was potentiated by EtOH. Thus, EtOH appears to produce potentiation via an alteration in the function of 5-HT3 receptors and not through an independent effect. gamma-Aminobutyric acidA receptor-mediated Cl- current was not potentiated by EtOH in neurons in which potentiation of responses to 5-HT was observed. Methanol potentiated 5-HT3 receptor- mediated current with a potency lower than that of EtOH. Potentiation by EtOH decreased with increasing 5-HT concentration. In addition, EtOH increased the decay rate of current. EtOH did not alter the reversal potential of the 5-HT3 receptor-mediated current. These observations indicate that intoxicating concentrations of EtOH selectively potentiate 5-HT3 receptor-mediated responses by increasing the apparent potency of 5-HT for activating ion current.
This article has been cited by other articles:
|
|
 |
|
  Z. A. Rodd, V. E. Gryszowka, J. E. Toalston, S. M. Oster, D. Ji, R. L. Bell, and W. J. McBride The Reinforcing Actions of a Serotonin-3 Receptor Agonist within the Ventral Tegmental Area: Evidence for Subregional and Genetic Differences and Involvement of Dopamine Neurons J. Pharmacol. Exp. Ther., June 1, 2007; 321(3): 1003 - 1012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Rusch, B. Musset, H. Wulf, A. Schuster, and D. E. Raines Subunit-Dependent Modulation of the 5-Hydroxytryptamine Type 3 Receptor Open-Close Equilibrium by n-Alcohols J. Pharmacol. Exp. Ther., June 1, 2007; 321(3): 1069 - 1074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Thielen, E. A. Engleman, Z. A. Rodd, J. M. Murphy, L. Lumeng, T.-K. Li, and W. J. McBride Ethanol Drinking and Deprivation Alter Dopaminergic and Serotonergic Function in the Nucleus Accumbens of Alcohol-Preferring Rats J. Pharmacol. Exp. Ther., April 1, 2004; 309(1): 216 - 225. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Wallner, H. J. Hanchar, and R. W. Olsen From The Cover: Ethanol enhances {alpha}4{beta}3{delta} and {alpha}6{beta}3{delta} {gamma}-aminobutyric acid type A receptors at low concentrations known to affect humans PNAS, December 9, 2003; 100(25): 15218 - 15223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Sessoms-Sikes, M. E. Hamilton, L.-X. Liu, D. M. Lovinger, and T. K. Machu A Mutation in Transmembrane Domain II of the 5-Hydroxytryptamine3A Receptor Stabilizes Channel Opening and Alters Alcohol Modulatory Actions J. Pharmacol. Exp. Ther., August 1, 2003; 306(2): 595 - 604. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhang, M. Hosoi, M. Fukuzawa, H. Sun, R. R. Rawlings, and F. F. Weight Distinct Molecular Basis for Differential Sensitivity of the Serotonin Type 3A Receptor to Ethanol in the Absence and Presence of Agonist J. Biol. Chem., November 22, 2002; 277(48): 46256 - 46264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. A. Hewlett, B. L. Trivedi, Z.-J. Zhang, T. de Paulis, D. E. Schmidt, D. M. Lovinger, M. S. Ansari, and M. H. Ebert Characterization of (S)-Des-4-amino-3-[125I]iodozacopride ([125I]DAIZAC), a Selective High-Affinity Radioligand for 5-Hydroxytryptamine3 Receptors J. Pharmacol. Exp. Ther., January 1, 1999; 288(1): 221 - 231. [Abstract] [Full Text]
|
|
 |
 |
 |
|
 |
 |
 |
