![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
KB Walsh
Department of Pharmacology, University of South Carolina, School of Medicine, Columbia 29208.
The whole-cell arrangement of the patch-clamp technique was used to examine the effect of protein kinase C (PKC) stimulation on ion channels in isolated guinea pig ventricular cells. In the presence of appropriate external solutions and drugs to reduce contamination from sodium, calcium, and potassium ion currents, application of phorbol 12- myristate 13-acetate or phorbol 12,13-dibutyrate, to stimulate PKC, activated a time-independent background current. The current-voltage relation for the PKC-activated current was linear over the voltage range of -90 to +60 mV. Alteration of the chloride equilibrium potential, brought about through changes in external and internal Cl- concentrations, shifted the reversal potential for the background current in a manner expected for a Cl- -selective ion channel. The PKC- activated current was reversibly inhibited by the S-(-)-enantiomer of the monocarboxylic acid derivative 8-chlorophenoxyproprionic acid, at a concentration that did not affect Ca2+ or delayed rectifier K+ currents. Dialysis of ventricular cells with partially purified PKC obtained from rat brain resulted in the activation of a large (greater than 1-nA) time-independent background current after addition of external phorbol 12,13-dibutyrate. In the presence of the beta- adrenergic receptor antagonist propranolol, norepinephrine activated a background current with properties similar to those of the PKC- sensitive current. It is concluded that cardiac ventricular cells contain PKC-activated Cl- channels, which may be regulated during alpha- adrenergic stimulation.
This article has been cited by other articles:
|
|
 |
|
  Y. Yashiro and B. R. Duling Integrated Ca2+ Signaling Between Smooth Muscle and Endothelium of Resistance Vessels Circ. Res., November 24, 2000; 87(11): 1048 - 1054. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. C. Britton, W. J. Hatton, C. F. Rossow, D. Duan, J. R. Hume, and B. Horowitz Molecular distribution of volume-regulated chloride channels (ClC-2 and ClC-3) in cardiac tissues Am J Physiol Heart Circ Physiol, November 1, 2000; 279(5): H2225 - H2233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Hume, D. Duan, M. L. Collier, J. Yamazaki, and B. Horowitz Anion Transport in Heart Physiol Rev, January 1, 2000; 80(1): 31 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sorota Insights into the structure, distribution and function of the cardiac chloride channels Cardiovasc Res, May 1, 1999; 42(2): 361 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hiraoka, S. Kawano, Y. Hirano, and T. Furukawa Role of cardiac chloride currents in changes in action potential characteristics and arrhythmias Cardiovasc Res, October 1, 1998; 40(1): 23 - 33. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Middleton and R. D. Harvey PKC regulation of cardiac CFTR Cl- channel function in guinea pig ventricular myocytes Am J Physiol Cell Physiol, July 1, 1998; 275(1): C293 - C302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Oleksa, L. C. Hool, and R. D. Harvey {alpha}1-Adrenergic Inhibition of the ß-Adrenergically Activated Cl- Current in Guinea Pig Ventricular Myocytes Circ. Res., June 1, 1996; 78(6): 1090 - 1099. [Abstract] [Full Text]
|
|
|
|
|
|
D. Duan, B. Fermini, and S. Nattel {alpha}-Adrenergic Control of Volume-Regulated Cl- Currents in Rabbit Atrial Myocytes : Characterization of a Novel Ionic Regulatory Mechanism Circ. Res., August 1, 1995; 77(2): 379 - 393. [Abstract] [Full Text]
|
|
|
|
|
|
M. L. Collier and J. R. Hume Unitary Chloride Channels Activated by Protein Kinase C in Guinea Pig Ventricular Myocytes Circ. Res., February 1, 1995; 76(2): 317 - 324. [Abstract] [Full Text]
|
|
 |
 |
 |
|
 |
 |
 |
