![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
DT Dudley, SE Hubbell and RM Summerfelt
Department of Signal Transduction, Parke-Davis Pharmaceutical Research Division, Warner-Lambert Company, Ann Arbor, Michigan 48105.
Binding sites for angiotensin II were found, in a line of Swiss 3T3 cells (designated as R3T3 cells), that were insensitive to Dup 753 and dithiothreitol yet were sensitive to PD 123319, making them members of the AT2 class of angiotensin II binding sites. These binding sites appeared not to be coupled to guanine nucleotide-binding proteins, and affinity labeling experiments revealed a specifically labeled protein with an apparent molecular weight of about 100,000. Treatment of cells with angiotensin II revealed no perturbation of common signaling pathways, including stimulation of phosphatidylinositol turnover, effects on levels of cAMP, tyrosine kinase activity, and release of arachidonic acid. Also, angiotensin II or PD 123319 had no effect on cell growth, mitogenesis, or hypertrophy or on mitogenesis or hypertrophy stimulated by several growth factors. These results show that the AT2 binding site is quite distinct from the AT1 site in terms of molecular weight, binding properties, and coupling to second messenger systems. Although the significance of this novel angiotensin II binding site remains obscure, the identification of cell lines selectively expressing it should greatly aid in the understanding of its regulation and function.
This article has been cited by other articles:
|
|
 |
|
  M. P. Schuijt, P. R. Saxena, A.H. J. Danser, R. M. Carey, N. L. Howell, X.-H. Jin, and H. M. Siragy No Net Effect of Angiotensin II on Blood Pressure? * Response Hypertension, May 1, 2002; 39 (5): e27 - e28. [Full Text] [PDF]
|
|
|
|
 |
|
 M. de Gasparo, K. J. Catt, T. Inagami, J. W. Wright, and Th. Unger International Union of Pharmacology. XXIII. The Angiotensin II Receptors Pharmacol. Rev., September 1, 2000; 52(3): 415 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gallinat, S. Busche, M. K. Raizada, and C. Sumners The angiotensin II type 2 receptor: an enigma with multiple variations Am J Physiol Endocrinol Metab, March 1, 2000; 278(3): E357 - E374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Horiuchi, W. Hayashida, M. Akishita, S. Yamada, J. Y. A. Lehtonen, K. Tamura, L. Daviet, Y. E. Chen, M. Hamai, T.-X. Cui, et al. Interferon-{gamma} Induces AT2 Receptor Expression in Fibroblasts by Jak/STAT Pathway and Interferon Regulatory Factor-1 Circ. Res., February 4, 2000; 86(2): 233 - 240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Gendron*, L. Laflamme*, N. Rivard, C. Asselin, M. D. Payet, and N. Gallo-Payet Signals from the AT2 (Angiotensin Type 2) Receptor of Angiotensin II Inhibit p21ras and Activate MAPK (Mitogen-Activated Protein Kinase) to Induce Morphological Neuronal Differentiation in NG108-15 Cells Mol. Endocrinol., September 1, 1999; 13(9): 1615 - 1626. [Abstract] [Full Text]
|
|
|
|
|
|
M. Horiuchi, M. Akishita, and V. J. Dzau Recent Progress in Angiotensin II Type 2 Receptor Research in the Cardiovascular System Hypertension, February 1, 1999; 33(2): 613 - 621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tamura, Y. Wanaka, E. J. Landon, and T. Inagami Intracellular Sodium Modulates the Expression of Angiotensin II Subtype 2 Receptor in PC12W Cells Hypertension, February 1, 1999; 33(2): 626 - 632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Horiuchi, W. Hayashida, T. Kambe, T. Yamada, and V. J. Dzau Angiotensin Type 2 Receptor Dephosphorylates Bcl-2 by Activating Mitogen-activated Protein Kinase Phosphatase-1 and Induces Apoptosis J. Biol. Chem., July 25, 1997; 272(30): 19022 - 19026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Horiuchi, T. Yamada, W. Hayashida, and V. J. Dzau Interferon Regulatory Factor-1 Up-regulates Angiotensin II Type 2 Receptor and Induces Apoptosis J. Biol. Chem., May 2, 1997; 272(18): 11952 - 11958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Balmforth, A. J. Lee, P. Warburton, D. Donnelly, and S. G. Ball The Conformational Change Responsible for AT1 Receptor Activation Is Dependent upon Two Juxtaposed Asparagine Residues on Transmembrane Helices III and VII J. Biol. Chem., February 14, 1997; 272(7): 4245 - 4251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. R. Huckle, M. D. Drag, W. R. Acker, M. Powers, R. C. McFall, D. J. Holder, T. Fujita, I. I. Stabilito, D. Kim, D. L. Ondeyka, et al. Effects of Subtype-Selective and Balanced Angiotensin II Receptor Antagonists in a Porcine Coronary Artery Model of Vascular Restenosis Circulation, March 1, 1996; 93(5): 1009 - 1019. [Abstract] [Full Text]
|
|
|
|
|
|
T. Ichiki, Y. Kambayashi, and T. Inagami Multiple Growth Factors Modulate mRNA Expression of Angiotensin II Type-2 Receptor in R3T3 Cells Circ. Res., December 1, 1995; 77(6): 1070 - 1076. [Abstract] [Full Text]
|
|
|
|
|
|
M. Horiuchi, G. Koike, T. Yamada, M. Mukoyama, M. Nakajima, and V. J. Dzau The Growth-dependent Expression of Angiotensin II Type 2 Receptor Is Regulated by Transcription Factors Interferon Regulatory Factor-1 and -2 J. Biol. Chem., August 25, 1995; 270(34): 20225 - 20230. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
