MolPharm xPharm- The Comprehensive Pharmacology Reference

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yun, Y. P.
Right arrow Articles by Song, B. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yun, Y. P.
Right arrow Articles by Song, B. J.

Pretranslational activation of cytochrome P450IIE during ketosis induced by a high fat diet

YP Yun, JP Casazza, DH Sohn, RL Veech and BJ Song

Laboratory of Metabolism and Molecular Biology, National Institute on Alcohol Abuse and Alcoholism, Rockville, Maryland 20852.

Ethanol-inducible cytochrome P450 (P450IIE) is reported to be induced by ketosis. In the present study, the effects of a high fat diet on P450IIE induction and the relationship between ketone body concentration and P450IIE induction were studied by the following: 1) measurement of the activity of aniline hydroxylase, 2) immunoblot analysis for P450IIE protein, and 3) Northern blot analysis for P450IIE mRNA. The enzyme activities (aniline hydroxylase) in hepatic and renal microsomes were elevated about 2-3-fold by feeding with a high fat diet for 3 days. The increases in enzyme activities were also accompanied by 3-fold increases in immunoreactive P450IIE protein and its mRNA. In contrast, no differences were observed for the catalytic activities of N-alkoxyresorufin dealkylases or the amounts of immunoreactive P450IA and P450IIC, indicating a specific induction of P450IIE by high fat feeding. Furthermore, the increases in the levels of P450IIE mRNA correlated positively (r = 0.73) with plasma concentrations of acetoacetate and beta-hydroxybutyrate but not with that of acetone, which induces P450IIE without changing its mRNA level. Our data thus indicated that P450IIE induction during the ketosis of a high fat feeding appears to be due to pretranslational activation and that is similar to the induction mechanism of fasted and diabetic animals.

Volume 41, Issue 3, pp. 474-479, 03/01/1992
Copyright © 1992 by American Society for Pharmacology and Experimental Therapeutics




This article has been cited by other articles:


Home page
 Toxicol SciHome page
J. L. Raucy, J. Lasker, K. Ozaki, and V. Zoleta
Regulation of CYP2E1 by Ethanol and Palmitic Acid and CYP4A11 by Clofibrate in Primary Cultures of Human Hepatocytes
Toxicol. Sci., June 1, 2004; 79(2): 233 - 241.
[Abstract] [Full Text] [PDF]

Home page
 Drug Metab. Dispos.Home page
H. C. Chung, S. H. Sung, J. S. Kim, Y. C. Kim, and S. G. Kim
Lack of Cytochrome P450 2E1 (CYP2E1) Induction in the Rat Liver by Starvation without Coprophagy
Drug Metab. Dispos., March 1, 2001; 29(3): 213 - 216.
[Abstract] [Full Text]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. B. Sewer, D. R. Koop, and E. T. Morgan
Differential Inductive and Suppressive Effects of Endotoxin and Particulate Irritants on Hepatic and Renal Cytochrome P-450 Expression
J. Pharmacol. Exp. Ther., March 1, 1997; 280(3): 1445 - 1454.
[Abstract] [Full Text]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1992 by the American Society for Pharmacology and Experimental Therapeutics