![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
JA Mitchell, KL Kohlhaas, T Matsumoto, JS Pollock, U Forstermann, TD Warner, HH Schmidt and F Murad
Department 47S, Abbott Laboratories, Abbott Park, Illinois 60064.
Lipopolysaccharide (LPS), either alone or in combination with cytokines, induces nitric oxide (NO) synthase activity in cells that normally release little or no NO. In arterial smooth muscle cells and various macrophage cell lines, NO synthase activity is induced after several hours of incubation with LPS. In brain, NADPH-dependent diaphorase activity has been associated with constitutive NO synthase. Here we show that incubation of rat aorta or cultured macrophages with LPS causes a time-dependent induction of NO synthase. The NO synthase activity in both rat aorta and macrophages was calcium independent and inhibited by NG-monomethyl-L-arginine and NG-nitro-L-arginine. We also found that LPS caused a time-dependent induction in NADPH-dependent diaphorase activity in both rat aorta and cultured macrophages. The diaphorase activity was mainly NADPH dependent and NADH independent. NO synthase activity and NADPH-diaphorase activity in crude cytosol from LPS-treated macrophages were found to co-purify, using 2',5'-ADP- Sepharose followed by Superose-6 gel permeation chromatography.
This article has been cited by other articles:
|
|
 |
|
  K. R. Hamal, R. Wideman, N. Anthony, and G. F. Erf Expression of Inducible Nitric Oxide Synthase in Lungs of Broiler Chickens Following Intravenous Cellulose Microparticle Injection Poult. Sci., April 1, 2008; 87(4): 636 - 644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Aga, J. J. Watters, Z. A. Pfeiffer, G. J. Wiepz, J. A. Sommer, and P. J. Bertics Evidence for nucleotide receptor modulation of cross talk between MAP kinase and NF-{kappa}B signaling pathways in murine RAW 264.7 macrophages Am J Physiol Cell Physiol, April 1, 2004; 286(4): C923 - C930. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Watters, J. A. Sommer, Z. A. Pfeiffer, U. Prabhu, A. N. Guerra, and P. J. Bertics A Differential Role for the Mitogen-activated Protein Kinases in Lipopolysaccharide Signaling. THE MEK/ERK PATHWAY IS NOT ESSENTIAL FOR NITRIC OXIDE AND INTERLEUKIN 1beta PRODUCTION J. Biol. Chem., March 8, 2002; 277(11): 9077 - 9087. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Gui, X.-L. Zheng, and M. D. Hollenberg Interleukin-1beta , Src- and non-Src tyrosine kinases, and nitric oxide synthase induction in rat aorta in vitro Am J Physiol Heart Circ Physiol, August 1, 2000; 279(2): H566 - H576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Schwarz, H. Kleinert, and U. Forstermann Potential Functional Significance of Brain-Type and Muscle-Type Nitric Oxide Synthase I Expressed in Adventitia and Media of Rat Aorta Arterioscler. Thromb. Vasc. Biol., November 1, 1999; 19(11): 2584 - 2590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Bjornheden, M. Levin, M. Evaldsson, and O. Wiklund Evidence of Hypoxic Areas Within the Arterial Wall In Vivo Arterioscler. Thromb. Vasc. Biol., April 1, 1999; 19(4): 870 - 876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Bjornheden, M. Evaldsson, and O. Wiklund A Method for the Assessment of Hypoxia in the Arterial Wall, With Potential Application In Vivo Arterioscler. Thromb. Vasc. Biol., January 1, 1996; 16(1): 178 - 185. [Abstract] [Full Text]
|
|
 |
 |
 |
|
 |
 |
 |
