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DM Rock and RL MacDonald
Neuroscience Program, University of Michigan, Ann Arbor 48104-1687.
Several polyamines have been shown to interact with a site on the N- methyl-D-aspartate (NMDA) receptor that regulates the binding of open channel blockers. Spermine (SP) and spermidine (SD), polyamine agonists, enhanced binding of open channel blockers, whereas arcaine (ARC), diethylenetriamine (DET), and putrescine (PUT), polyamine antagonists, reduced the polyamine enhancement of open channel blocker binding. We previously showed that SP had multiple actions on NMDA receptor single-channel currents that underlie its effect on whole-cell NMDA receptor current. At high concentrations, SP produced a voltage- dependent decrease in NMDA receptor single-channel conductance and average open time. In the present study, another polyamine agonist (SD) produced a similar reduction of NMDA receptor single-channel conductance at higher concentrations. The polyamine antagonists (ARC, DET, and PUT), however, produced a voltage-dependent reduction in NMDA receptor whole-cell currents and reductions in single-channel conductance and average open time, even in the absence of polyamine agonists. The rank order of potency for reduction of NMDA receptor single-channel conductance by polyamines was ARC greater than SP greater than SD greater than PUT = DET, a rank order similar to that for the inhibitory actions of polyamines in receptor binding assays and for the effects of the antagonists on NMDA receptor whole-cell currents. The polyamine antagonist DET did not block the reduction of single-channel conductance by the polyamine agonist SP. In fact, the effects of SP and DET on single-channel conductance were additive. DET also showed a variable enhancement of NMDA receptor whole-cell currents in some neurons, suggesting polyamine agonist-like properties. These results are not consistent with the standard pharmacological profile for agonists and antagonists acting at the same site. Potential mechanisms for the effects of the polyamines on single-channel conductance are discussed.
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