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Calcium channel current in cultured rat mesangial cells

M Nishio, H Tsukahara, M Hiraoka, M Sudo, S Kigoshi and I Muramatsu

Department of Pharmacology, Fukui Medical School, Japan.

The presence of voltage-dependent calcium channels has been suggested in mesangial cells by using calcium-sensitive fluorescent probes. However, direct electrophysiological evidence for voltage-dependent calcium channels has not yet been presented. In this study voltage- dependent calcium channels were studied in cultured rat mesangial cells. Whole-cell patch-clamp experiments were done with 50 mM Ba2+ as a charge carrier. Step depolarizing pulses from a holding potential of - 50 mV produced an inward barium current at potentials more positive than -10 mV, and a peak current (10-45 pA) was obtained at a membrane potential of approximately +30 mV. The inward current was augmented by 100 nM Bay K 8644, attenuated by 1 microM nifedipine, and abolished by 50 microM Cd2+. These results indicate that the inward current is a barium current flowing through L-type calcium channels. This may be the first study that demonstrates the presence of L-type calcium channels in mesangial cells.

Volume 43, Issue 1, pp. 96-99, 01/01/1993
Copyright © 1993 by American Society for Pharmacology and Experimental Therapeutics




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