![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
D Gurantz, JF Margiotta, AT Harootunian and VE Dionne
Department of Pharmacology, University of California, San Diego, La Jolla 92093-0636.
Bovine serum albumin greatly enhanced the cholinergic response mediated by neuronal nicotinic acetylcholine receptors in chick ciliary ganglion neurons. The enhancement exceeded 5-fold in some experiments (mean +/- standard error, 3.26 +/- 0.43-fold) and was rapid, was dose dependent, and occurred without changes in the unitary conductance or the mean open time of the acetylcholine receptor channel. This lack of detectable change in permeation or kinetic properties suggests that bovine serum albumin might increase acetylcholine responses by increasing the number of functional receptors. The enhancement appears to be specific to the albumin molecule, because activity could not be removed by detergent extraction, gel filtration, or dialysis. Acetylcholine responses in these cells are known to be enhanced by a cAMP-dependent mechanism that converts existing acetylcholine receptors from a nonfunctional to a functional state. We found that the enhancement by bovine serum albumin occurred without an increase in cAMP and that pretreatment with membrane-permeable cAMP analogs prevented any additional enhancement of the cholinergic response by bovine serum albumin. These observations are consistent with a cAMP- dependent modulation of the enhancement produced by bovine serum albumin or a convergence of the two enhancement mechanisms onto a single pathway.
This article has been cited by other articles:
|
|
 |
|
  D. B. Freir and C. E. Herron Nicotine Enhances the Depressive Actions of A{beta}1-40 on Long-Term Potentiation in the Rat Hippocampal CA1 Region In Vivo J Neurophysiol, June 1, 2003; 89(6): 2917 - 2922. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. G. Conroy, Q.-S. Liu, Q. Nai, J. F. Margiotta, and D. K. Berg Potentiation of alpha 7-Containing Nicotinic Acetylcholine Receptors by Select Albumins Mol. Pharmacol., February 1, 2003; 63(2): 419 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Dobelis, M. J. Marks, P. Whiteaker, S. A. Balogh, A. C. Collins, and J. A. Stitzel A Polymorphism in the Mouse Neuronal alpha 4 Nicotinic Receptor Subunit Results in An Alteration in Receptor Function Mol. Pharmacol., August 1, 2002; 62(2): 334 - 342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Abou-Donia, A. M. Dechkovskaia, L. B. Goldstein, S. L. Bullman, and W. A. Khan Sensorimotor Deficit and Cholinergic Changes following Coexposure with Pyridostigmine Bromide and Sarin in Rats Toxicol. Sci., March 1, 2002; 66(1): 148 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. A. Khan, A. M. Dechkovskaia, E. A. Herrick, K. H. Jones, and M. B. Abou-Donia Acute Sarin Exposure Causes Differential Regulation of Choline Acetyltransferase, Acetylcholinesterase, and Acetylcholine Receptors in the Central Nervous System of the Rat Toxicol. Sci., September 1, 2000; 57(1): 112 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. Fenster, M. L. Beckman, J. C. Parker, E. B. Sheffield, T. L. Whitworth, M. W. Quick, and R. A. J. Lester Regulation of alpha 4beta 2 Nicotinic Receptor Desensitization by Calcium and Protein Kinase C Mol. Pharmacol., March 1, 1999; 55(3): 432 - 443. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. M. Blumenthal, W. G. Conroy, S. J. Romano, P. D. Kassner, and D. K. Berg Detection of Functional Nicotinic Receptors Blocked by alpha -Bungarotoxin on PC12 Cells and Dependence of Their Expression on Post-Translational Events J. Neurosci., August 15, 1997; 17(16): 6094 - 6104. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
