![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
L Counillon, W Scholz, HJ Lang and J Pouyssegur
Centre de Biochimie, Universite de Nice-Sophia Antipolis.
A fibroblast mutant cell line devoid of Na+/H+ exchange was used to stably express cDNAs encoding the NHE1, NHE2, and NHE3 Na+/H+ antiporters. Pharmacological studies using amiloride and two of its 5-N- substituted derivatives, 5-N-dimethyl amiloride and 5-N-(methyl- propyl)amiloride (MPA), demonstrate that the NHE1 isoform is the ubiquitously expressed amiloride-sensitive Na+/H+ antiporter (Ki of 0.08 microM for MPA), whereas the NHE2 and NHE3 isoforms exhibit a lower affinity for these inhibitors (Ki of 0.5 microM and 10 microM, respectively, for MPA) and are therefore likely to be members of the epithelial Na+/H+ exchanger's family. In addition, we have used this system to test a new Na+/H+ exchanger inhibitor possessing anti- ischemic properties on myocardial cells [(3-methylsulphonyl-4- piperidinobenzoyl) guanidine methanesulphonate]. This compound inhibits competitively NHE1 (Ki of 0.16 microM) with a much greater affinity than NHE2 and NHE3 (Ki of 5 microM and 650 microM, respectively) and therefore appears to be much more discriminative between these two classes of antiporter isoforms than the amiloride-related molecules. These results suggest an explanation for the observed difference of physiological effects between amiloride and HOE694, and identify this new inhibitor as a useful tool for studies of Na+/H+ exchange.
This article has been cited by other articles:
|
|
 |
|
  H. Hayashi, O. Aharonovitz, R. T. Alexander, N. Touret, W. Furuya, J. Orlowski, and S. Grinstein Na+/H+ exchange and pH regulation in the control of neutrophil chemokinesis and chemotaxis Am J Physiol Cell Physiol, February 1, 2008; 294(2): C526 - C534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-S. Jung, H.-Y. Kim, J. Kim, M.-G. Lee, J. Pouyssegur, and E. Kim Physical Interactions and Functional Coupling between Daxx and Sodium Hydrogen Exchanger 1 in Ischemic Cell Death J. Biol. Chem., January 11, 2008; 283(2): 1018 - 1025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. D. Johnstone, P. F. Speake, and C. P. Sibley Epidermal growth factor and sphingosine-1-phosphate stimulate Na+/H+ exchanger activity in the human placental syncytiotrophoblast Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2007; 293(6): R2290 - R2294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Subramanya, V. M. Rajendran, P. Srinivasan, N. S. Nanda Kumar, B. S. Ramakrishna, and H. J. Binder Differential regulation of cholera toxin-inhibited Na-H exchange isoforms by butyrate in rat ileum Am J Physiol Gastrointest Liver Physiol, October 1, 2007; 293(4): G857 - G863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. Pedersen, S. A. King, E. B. Nygaard, R. R. Rigor, and P. M. Cala NHE1 Inhibition by Amiloride- and Benzoylguanidine-type Compounds: INHIBITOR BINDING LOCI DEDUCED FROM CHIMERAS OF NHE1 HOMOLOGUES WITH ENDOGENOUS DIFFERENCES IN INHIBITOR SENSITIVITY J. Biol. Chem., July 6, 2007; 282(27): 19716 - 19727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mason, G. V. Mallo, M. R. Terebiznik, B. Payrastre, B. B. Finlay, J. H. Brumell, L. Rameh, and S. Grinstein Alteration of Epithelial Structure and Function Associated with PtdIns(4,5)P2 Degradation by a Bacterial Phosphatase J. Gen. Physiol., March 26, 2007; 129(4): 267 - 283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. T. Alexander, A. Malevanets, A. M. Durkan, H. S. Kocinsky, P. S. Aronson, J. Orlowski, and S. Grinstein Membrane Curvature Alters the Activation Kinetics of the Epithelial Na+/H+ Exchanger, NHE3 J. Biol. Chem., March 9, 2007; 282(10): 7376 - 7384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. R. Carraro-Lacroix, M. A. Ramirez, T. M. T. Zorn, N. A. Reboucas, and G. Malnic Increased NHE1 expression is associated with serum deprivation-induced differentiation in immortalized rat proximal tubule cells Am J Physiol Renal Physiol, July 1, 2006; 291(1): F129 - F139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yamanishi, K. Katsumura, T. Kobayashi, and D. G. Puro Extracellular lactate as a dynamic vasoactive signal in the rat retinal microvasculature Am J Physiol Heart Circ Physiol, March 1, 2006; 290(3): H925 - H934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Pastor-Soler, C. Pietrement, and S. Breton Role of Acid/Base Transporters in the Male Reproductive Tract and Potential Consequences of Their Malfunction Physiology, December 1, 2005; 20(6): 417 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A Bailey, G. Giebisch, T. Abbiati, P. S Aronson, L. R Gawenis, G. E Shull, and T. Wang NHE2-mediated bicarbonate reabsorption in the distal tubule of NHE3 null mice J. Physiol., December 15, 2004; 561(3): 765 - 775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. C. Girardi, F. Knauf, H.-U. Demuth, and P. S. Aronson Role of dipeptidyl peptidase IV in regulating activity of Na+/H+ exchanger isoform NHE3 in proximal tubule cells Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1238 - C1245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Fidzinski, M. Salvador-Silva, L. Choritz, J. Geibel, and M. Coca-Prados Inhibition of NHE-1 Na+/H+ exchanger by natriuretic peptides in ocular nonpigmented ciliary epithelium Am J Physiol Cell Physiol, September 1, 2004; 287(3): C655 - C663. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhao, H. Shiue, S. Palkon, Y. Wang, P. Cullinan, J. K. Burkhardt, M. W. Musch, E. B. Chang, and J. R. Turner Ezrin regulates NHE3 translocation and activation after Na+-glucose cotransport PNAS, June 22, 2004; 101(25): 9485 - 9490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chen, C. X. Chen, X. T. Gan, N. Beier, W. Scholz, and M. Karmazyn Inhibition and reversal of myocardial infarction-induced hypertrophy and heart failure by NHE-1 inhibition Am J Physiol Heart Circ Physiol, January 1, 2004; 286(1): H381 - H387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. K. Putney and D. L. Barber Na-H Exchange-dependent Increase in Intracellular pH Times G2/M Entry and Transition J. Biol. Chem., November 7, 2003; 278(45): 44645 - 44649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Krishnan, V. M. Rajendran, and H. J. Binder Apical NHE isoforms differentially regulate butyrate-stimulated Na absorption in rat distal colon Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1246 - C1254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Behne, J. W. Meyer, K. M. Hanson, N. P. Barry, S. Murata, D. Crumrine, R. W. Clegg, E. Gratton, W. M. Holleran, P. M. Elias, et al. NHE1 Regulates the Stratum Corneum Permeability Barrier Homeostasis. MICROENVIRONMENT ACIDIFICATION ASSESSED WITH FLUORESCENCE LIFETIME IMAGING J. Biol. Chem., November 27, 2002; 277(49): 47399 - 47406. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.A. Harding, C.A. Gibb, M.H. Johnson, D.I. Cook, and M.L. Day Developmental Changes in the Management of Acid Loads During Preimplantation Mouse Development Biol Reprod, November 1, 2002; 67(5): 1419 - 1429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chu, S. Chu, and M. H. Montrose Apical Na+/H+ exchange near the base of mouse colonic crypts Am J Physiol Cell Physiol, July 1, 2002; 283(1): C358 - C372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Mitchell, J. C. Fleischhauer, W. D. Stamer, K. Peterson-Yantorno, and M. M. Civan Human trabecular meshwork cell volume regulation Am J Physiol Cell Physiol, July 1, 2002; 283(1): C315 - C326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Y. Avila, R. W. Seidler, R. A. Stone, and M. M. Civan Inhibitors of NHE-1 Na+/H+ Exchange Reduce Mouse Intraocular Pressure Invest. Ophthalmol. Vis. Sci., June 1, 2002; 43(6): 1897 - 1902. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Yun, Y. Chen, and F. Lang Glucocorticoid Activation of Na+/H+ Exchanger Isoform 3 Revisited. THE ROLES OF SGK1 AND NHERF2 J. Biol. Chem., March 1, 2002; 277(10): 7676 - 7683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yang, A. Hodel, and G. D. Holman Insulin and Isoproterenol Have Opposing Roles in the Maintenance of Cytosol pH and Optimal Fusion of GLUT4 Vesicles with the Plasma Membrane J. Biol. Chem., February 15, 2002; 277(8): 6559 - 6566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wang, M. Hropot, P. S. Aronson, and G. Giebisch Role of NHE isoforms in mediating bicarbonate reabsorption along the nephron Am J Physiol Renal Physiol, December 1, 2001; 281(6): F1117 - F1122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Khadilkar, P. Iannuzzi, and J. Orlowski Identification of Sites in the Second Exomembrane Loop and Ninth Transmembrane Helix of the Mammalian Na+/H+ Exchanger Important for Drug Recognition and Cation Translocation J. Biol. Chem., November 16, 2001; 276(47): 43792 - 43800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Turner and E. D. Black NHE3-dependent cytoplasmic alkalinization is triggered by Na+-glucose cotransport in intestinal epithelia Am J Physiol Cell Physiol, November 1, 2001; 281(5): C1533 - C1541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Chambrey, P. L. St. John, D. Eladari, F. Quentin, D. G. Warnock, D. R. Abrahamson, R.-A. Podevin, and M. Paillard Localization and functional characterization of Na+/H+ exchanger isoform NHE4 in rat thick ascending limbs Am J Physiol Renal Physiol, October 1, 2001; 281(4): F707 - F717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Xu, J. F. Collins, L. Bai, P. R. Kiela, R. M. Lynch, and F. K. Ghishan Epidermal growth factor regulation of rat NHE2 gene expression Am J Physiol Cell Physiol, August 1, 2001; 281(2): C504 - C513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. KARUMANCHI, L. JIANG, B. KNEBELMANN, A. K. STUART-TILLEY, S. L. ALPER, and V. P. SUKHATME VHL tumor suppressor regulates Cl-/HCO3- exchange and Na+/H+ exchange activities in renal carcinoma cells Physiol Genomics, April 2, 2001; 5(3): 119 - 128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Knight, A. H. Smith, D. M. Flynn, J. T. MacAndrew, S. S. Ellery, J. X. Kong, R. B. Marala, R. T. Wester, A. Guzman-Perez, R. J. Hill, et al. A Novel Sodium-Hydrogen Exchanger Isoform-1 Inhibitor, Zoniporide, Reduces Ischemic Myocardial Injury in Vitro and in Vivo J. Pharmacol. Exp. Ther., April 1, 2001; 297(1): 254 - 259. [Abstract] [Full Text]
|
|
|
|
|
|
V. M. Rajendran, J. Geibel, and H. J. Binder Characterization of apical membrane Cl-dependent Na/H exchange in crypt cells of rat distal colon Am J Physiol Gastrointest Liver Physiol, March 1, 2001; 280(3): G400 - G405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. De La Horra, M. Cano, M. J. Peral, M. L. Calonge, and A. A. Ilundain Hormonal regulation of chicken intestinal NHE and SGLT-1 activities Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2001; 280(3): R655 - R660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bagnis, M. Marsolais, D. Biemesderfer, R. Laprade, and S. Breton Na+/H+-exchange activity and immunolocalization of NHE3 in rat epididymis Am J Physiol Renal Physiol, March 1, 2001; 280(3): F426 - F436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.P.H. Leung, C.M. Tse, S.B. Cheng Chew, and P.Y.D. Wong Expression of Multiple Na+/H+ Exchanger Isoforms in Cultured Epithelial Cells from Rat Efferent Duct and Cauda Epididymidis Biol Reprod, February 1, 2001; 64(2): 482 - 490. [Abstract] [Full Text]
|
|
|
|
|
|
J. R. Turner, E. D. Black, J. Ward, C.-M. Tse, F. A. Uchwat, H. A. Alli, M. Donowitz, J. L. Madara, and J. M. Angle Transepithelial resistance can be regulated by the intestinal brush-border Na+/H+ exchanger NHE3 Am J Physiol Cell Physiol, December 1, 2000; 279(6): C1918 - C1924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X-H. Xiao and D.G. Allen Activity of the Na+/H+ exchanger is critical to reperfusion damage and preconditioning in the isolated rat heart Cardiovasc Res, November 1, 2000; 48(2): 244 - 253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-J. Rupprecht, J. v. Dahl, W. Terres, K. M. Seyfarth, G. Richardt, H.-P. Schulthei{beta}, M. Buerke, F. H. Sheehan, and H. Drexler Cardioprotective Effects of the Na+/H+ Exchange Inhibitor Cariporide in Patients With Acute Anterior Myocardial Infarction Undergoing Direct PTCA Circulation, June 27, 2000; 101(25): 2902 - 2908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Kiela, Y. S. Guner, H. Xu, J. F. Collins, and F. K. Ghishan Age- and tissue-specific induction of NHE3 by glucocorticoids in the rat small intestine Am J Physiol Cell Physiol, April 1, 2000; 278(4): C629 - C637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. McLean, J. Roscoe, N. K. Jorgensen, F. A. Gorin, and P. M. Cala Malignant gliomas display altered pH regulation by NHE1 compared with nontransformed astrocytes Am J Physiol Cell Physiol, April 1, 2000; 278(4): C676 - C688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Peti-Peterdi, R. Chambrey, Z. Bebok, D. Biemesderfer, P. L. St. John, D. R. Abrahamson, D. G. Warnock, and P. D. Bell Macula densa Na+/H+ exchange activities mediated by apical NHE2 and basolateral NHE4 isoforms Am J Physiol Renal Physiol, March 1, 2000; 278(3): F452 - F463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Z. Szabo, M. Numata, G. E. Shull, and J. Orlowski Kinetic and Pharmacological Properties of Human Brain Na+/H+ Exchanger Isoform 5 Stably Expressed in Chinese Hamster Ovary Cells J. Biol. Chem., February 25, 2000; 275(9): 6302 - 6307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Counillon and J. Pouyssegur The Expanding Family of Eucaryotic Na+/H+ Exchangers J. Biol. Chem., January 7, 2000; 275(1): 1 - 4. [Full Text] [PDF]
|
|
|
|
|
|
R. J. Gumina, E. Buerger, C. Eickmeier, J. Moore, J. Daemmgen, and G. J. Gross Inhibition of the Na+/H+ Exchanger Confers Greater Cardioprotection Against 90 Minutes of Myocardial Ischemia Than Ischemic Preconditioning in Dogs Circulation, December 21, 1999; 100(25): 2519 - 2526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Janecki, M. H. Montrose, C. M. Tse, F. S. de Medina, A. Zweibaum, and M. Donowitz Development of an endogenous epithelial Na+/H+ exchanger (NHE3) in three clones of Caco-2 cells Am J Physiol Gastrointest Liver Physiol, August 1, 1999; 277(2): G292 - G305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wang, C.-L. Yang, T. Abbiati, P. J. Schultheis, G. E. Shull, G. Giebisch, and P. S. Aronson Mechanism of proximal tubule bicarbonate absorption in NHE3 null mice Am J Physiol Renal Physiol, August 1, 1999; 277(2): F298 - F302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Aharonovitz, N. Demaurex, M. Woodside, and S. Grinstein ATP dependence is not an intrinsic property of Na+/H+ exchanger NHE1: requirement for an ancillary factor Am J Physiol Cell Physiol, June 1, 1999; 276(6): C1303 - C1311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. McLean, S. Zia, F. A. Gorin, and P. M. Cala Cloning and expression of the Na+/H+ exchanger from Amphiuma RBCs: resemblance to mammalian NHE1 Am J Physiol Cell Physiol, May 1, 1999; 276(5): C1025 - C1037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Bhartur, L. J. Ballarin, M. W. Musch, C. Bookstein, E. B. Chang, and M. C. Rao A unique Na+/H+ exchanger, analogous to NHE1, in the chicken embryonic fibroblast Am J Physiol Regulatory Integrative Comp Physiol, March 1, 1999; 276(3): R838 - R846. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ikuma, M. Kashgarian, H. J. Binder, and V. M. Rajendran Differential regulation of NHE isoforms by sodium depletion in proximal and distal segments of rat colon Am J Physiol Gastrointest Liver Physiol, February 1, 1999; 276(2): G539 - G549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Gonda, D. Maouyo, S. E. Rees, and M. H. Montrose Regulation of intracellular pH gradients by identified Na/H exchanger isoforms and a short-chain fatty acid Am J Physiol Gastrointest Liver Physiol, January 1, 1999; 276(1): G259 - G270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Laghmani, R. Chambrey, M. Froissart, M. Bichara, M. Paillard, and P. Borensztein Adaptation of NHE-3 in the rat thick ascending limb: effects of high sodium intake and metabolic alkalosis Am J Physiol Renal Physiol, January 1, 1999; 276(1): F18 - F26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lane, J. M. Baltz, and B. D. Bavister Regulation of Intracellular pH in Hamster Preimplantation Embryos by theSodium Hydrogen (Na+/H+) Antiporter Biol Reprod, December 1, 1998; 59(6): 1483 - 1490. [Abstract] [Full Text]
|
|
|
|
|
|
R. L. McSwine, M. W. Musch, C. Bookstein, Y. Xie, M. Rao, and E. B. Chang Regulation of apical membrane Na+/H+ exchangers NHE2 and NHE3 in intestinal epithelial cell line C2/bbe Am J Physiol Cell Physiol, September 1, 1998; 275(3): C693 - C701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. O. Andersen, M. Enger, G. H. Thoresen, T. Skomedal, and J.-B. Osnes alpha 1-Adrenergic activation of myocardial Na-K-2Cl cotransport involving mitogen-activated protein kinase Am J Physiol Heart Circ Physiol, August 1, 1998; 275(2): H641 - H652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Gumina, T. Mizumura, N. Beier, P. Schelling, J. J. Schultz, and G. J. Gross A New Sodium/Hydrogen Exchange Inhibitor, EMD 85131, Limits Infarct Size in Dogs When Administered Before or After Coronary Artery Occlusion J. Pharmacol. Exp. Ther., July 1, 1998; 286(1): 175 - 183. [Abstract] [Full Text]
|
|
|
|
|
|
M. Donowitz, C. De La Horra, M. L. Calonge, I. S. Wood, J. Dyer, S. M. Gribble, F. S. De Medina, C. M. Tse, S. P. Shirazi-Beechey, and A. A. Ilundain In birds, NHE2 is major brush-border Na+/H+ exchanger in colon and is increased by a low-NaCl diet Am J Physiol Regulatory Integrative Comp Physiol, June 1, 1998; 274(6): R1659 - R1669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wormmeester, F. S. De Medina, F. Kokke, C.-M. Tse, S. Khurana, J. Bowser, M. E. Cohen, and M. Donowitz Quantitative contribution of NHE2 and NHE3 to rabbit ileal brush-border Na+/H+ exchange Am J Physiol Cell Physiol, May 1, 1998; 274(5): C1261 - C1272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Janecki, M. H. Montrose, P. Zimniak, A. Zweibaum, C. M. Tse, S. Khurana, and M. Donowitz Subcellular Redistribution Is Involved in Acute Regulation of the Brush Border Na+/H+ Exchanger Isoform 3 in Human Colon Adenocarcinoma Cell Line Caco-2. PROTEIN KINASE C-MEDIATED INHIBITION OF THE EXCHANGER J. Biol. Chem., April 10, 1998; 273(15): 8790 - 8798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Cho, M. W. Musch, C. M. Bookstein, R. L. McSwine, K. Rabenau, and E. B. Chang Aldosterone stimulates intestinal Na+ absorption in rats by increasing NHE3 expression of the proximal colon Am J Physiol Cell Physiol, March 1, 1998; 274(3): C586 - C594. [Abstract] [Full Text] [PDF]
|
|
