![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
SR Nash, N Godinot and MG Caron
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710.
Opossum kidney cells are an established epithelial cell line which is often studied as a physiological model system of renal proximal tubule function, and which has also been shown to possess dopamine receptors. To identify dopamine receptor subtypes present in renal tissue, as well as to explore the usefulness of opossum kidney cells for the study of D1 dopamine receptors and renal dopaminergic physiology, we have undertaken the cloning and characterization of the dopamine receptor expressed in this cell line. In the brains of rats and humans, two different subtypes of D1 dopamine receptors, D1A and D1B, have recently been characterized. The OK cell D1 receptor message is 4500 bp long and exhibits extensive homology with the rat and human D1A subtypes of dopamine receptors. Pharmacological experiments were performed on COS-7 cell membranes transiently transfected with this cDNA. Binding properties were compared with those reported for OK cell membranes, and comparison experiments were performed in parallel with the human D1A expressed transiently in the same system. Molecular techniques including Northern blotting, in situ hybridization, and RNase protection analysis were used to study the expression pattern of the OK cell D1 receptor message. Expression of both D1A and D1B subtypes was detected in both the opossum brain and the opossum kidney, however, the OK cell line expresses exclusively the D1A receptor subtype.
This article has been cited by other articles:
|
|
 |
|
  A. R. Cinelli, R. Efendiev, and C. H. Pedemonte Trafficking of Na-K-ATPase and dopamine receptor molecules induced by changes in intracellular sodium concentration of renal epithelial cells Am J Physiol Renal Physiol, October 1, 2008; 295(4): F1117 - F1125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Efendiev, C. E. Budu, A. M. Bertorello, and C. H. Pedemonte G-protein-coupled Receptor-mediated Traffic of Na,K-ATPase to the Plasma Membrane Requires the Binding of Adaptor Protein 1 to a Tyr-255-based Sequence in the {alpha}-Subunit J. Biol. Chem., June 20, 2008; 283(25): 17561 - 17567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Zeng, I. Armando, Y. Luo, G. M. Eisner, R. A. Felder, and P. A. Jose Dysregulation of dopamine-dependent mechanisms as a determinant of hypertension: studies in dopamine receptor knockout mice Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H551 - H569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Zeng, H. Sanada, H. Watanabe, G. M. Eisner, R. A. Felder, and P. A. Jose Functional genomics of the dopaminergic system in hypertension Physiol Genomics, November 17, 2004; 19(3): 233 - 246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Efendiev, C. E. Budu, A. R. Cinelli, A. M. Bertorello, and C. H. Pedemonte Intracellular Na+ Regulates Dopamine and Angiotensin II Receptors Availability at the Plasma Membrane and Their Cellular Responses in Renal Epithelia J. Biol. Chem., August 1, 2003; 278(31): 28719 - 28726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hussain and M. F. Lokhandwala Renal Dopamine Receptors and Hypertension Experimental Biology and Medicine, February 1, 2003; 228(2): 134 - 142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. LUIPPOLD, C. ZIMMERMANN, M. MAI, D. KLOOR, D. STARCK, G. GROSS, and B. MUHLBAUER Dopamine D3 Receptors and Salt-Dependent Hypertension J. Am. Soc. Nephrol., November 1, 2001; 12(11): 2272 - 2279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Feraille and A. Doucet Sodium-Potassium-Adenosinetriphosphatase-Dependent Sodium Transport in the Kidney: Hormonal Control Physiol Rev, January 1, 2001; 81(1): 345 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Luippold, S. Schneider, V. Vallon, H. Osswald, and B. Muhlbauer Postglomerular vasoconstriction induced by dopamine D3 receptor activation in anesthetized rats Am J Physiol Renal Physiol, April 1, 2000; 278(4): F570 - F575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. C. Lembo, M. H. Ghahremani, and P. R. Albert Receptor Selectivity of the Cloned Opossum G Protein-Coupled Receptor Kinase 2 (GRK2) in Intact Opossum Kidney Cells: Role in Desensitization of Endogenous {alpha}2C-Adrenergic but Not Serotonin 1B Receptors Mol. Endocrinol., January 1, 1999; 13(1): 138 - 147. [Abstract] [Full Text]
|
|
|
|
 |
|
 Z.-Q. Wang, R. A. Felder, and R. M. Carey Selective Inhibition of the Renal Dopamine Subtype D1A Receptor Induces Antinatriuresis in Conscious Rats Hypertension, January 1, 1999; 33(1): 504 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Jose, L. D. Asico, G. M. Eisner, F. Pocchiari, C. Semeraro, and R. A. Felder Effects of costimulation of dopamine D1- and D2-like receptors on renal function Am J Physiol Regulatory Integrative Comp Physiol, October 1, 1998; 275(4): R986 - R994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hussain and M. F. Lokhandwala Renal Dopamine Receptor Function in Hypertension Hypertension, August 1, 1998; 32(2): 187 - 197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. MISSALE, S. R. NASH, S. W. ROBINSON, M. JABER, and M. G. CARON Dopamine Receptors: From Structure to Function Physiol Rev, January 1, 1998; 78(1): 189 - 225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. P. O'Connell, A. M. Aherne, E. Lane, R. A. Felder, and R. M. Carey Detection of dopamine receptor D1A subtype-specific mRNA in rat kidney by in situ amplification Am J Physiol Renal Physiol, January 1, 1998; 274(1): F232 - F241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. H. Pedemonte, T. A. Pressley, A. R. Cinelli, and M. F. Lokhandwala Stimulation of Protein Kinase C Rapidly Reduces Intracellular Na+ Concentration via Activation of the Na+ Pump in OK Cells Mol. Pharmacol., July 1, 1997; 52(1): 88 - 97. [Abstract] [Full Text]
|
|
|
|
|
|
I. Yamaguchi, L. Yao, H. Sanada, R. Ozono, M. M. Mouradian, P. A. Jose, R. M. Carey, and R. A. Felder Dopamine D1A Receptors and Renin Release in Rat Juxtaglomerular Cells Hypertension, April 1, 1997; 29(4): 962 - 968. [Abstract] [Full Text]
|
|
|
|
|
|
L. L. Demchyshyn, K. S. Sugamori, F. J. S. Lee, S. A. Hamadanizadeh, and H. B. Niznik The Dopamine D1D Receptor J. Biol. Chem., February 24, 1995; 270(8): 4005 - 4012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yang, C. K. Hwang, U. M. D'Souza, S.-H. Lee, E. Junn, and M. M. Mouradian Three-amino acid Extension Loop Homeodomain Proteins Meis2 and TGIF Differentially Regulate Transcription J. Biol. Chem., June 30, 2000; 275(27): 20734 - 20741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ogimoto, G. A. Yudowski, C. J. Barker, M. Kohler, A. I. Katz, E. Feraille, C. H. Pedemonte, P.-O. Berggren, and A. M. Bertorello G protein-coupled receptors regulate Na+,K+-ATPase activity and endocytosis by modulating the recruitment of adaptor protein 2 and clathrin PNAS, March 28, 2000; 97(7): 3242 - 3247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Yudowski, R. Efendiev, C. H. Pedemonte, A. I. Katz, P.-O. Berggren, and A. M. Bertorello Phosphoinositide-3 kinase binds to a proline-rich motif in the Na+,K+-ATPase alpha subunit and regulates its trafficking PNAS, June 6, 2000; 97(12): 6556 - 6561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Gomes and P. Soares-da-Silva Role of cAMP-PKA-PLC signaling cascade on dopamine-induced PKC-mediated inhibition of renal Na+-K+-ATPase activity Am J Physiol Renal Physiol, June 1, 2002; 282(6): F1084 - F1096. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
