![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
DT Price, RJ Lefkowitz, MG Caron, D Berkowitz and DA Schwinn
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710.
alpha 1-Adrenergic receptors (alpha 1ARs) are virtually ubiquitous in human tissues and mediate important physiological functions as diverse as smooth muscle contraction, glycogenolysis, and myocardial inotropy. At least three alpha 1AR subtypes (alpha 1A/D, alpha 1B, and alpha 1C) have been described using molecular and pharmacological techniques. The identification of species heterogeneity (rat versus rabbit) in alpha 1AR subtype distribution has made it imperative to determine the distribution of alpha 1AR subtypes in human tissues. Accordingly, RNA extracted from human tissues was analyzed using RNase protection assays to determine alpha 1AR subtype expression. Of the cloned alpha 1ARs, alpha 1CAR mRNA predominates in many human tissues (heart, liver, cerebellum, and cerebral cortex), in contrast to its restricted distribution in both rats and rabbits. alpha (1B)AR mRNA is present in highest concentrations in human spleen, kidney, and fetal brain. alpha 1A/DAR mRNA is present in highest concentrations in human aorta and cerebral cortex. Hence, alpha 1AR subtype mRNA distribution is tissue selective and differs from that reported for rats and rabbits. These results have potentially significant implications for understanding human adrenergic physiology and are important for the rational development of alpha 1AR subtype-selective drugs.
This article has been cited by other articles:
|
|
 |
|
  M. Stafford-Smith, R. Bartz, K. Wilson, J. N. Baraniuk, and D. A. Schwinn Alpha-adrenergic mRNA subtype expression in the human nasal turbinate: [Expression du sous-type d'ARN messager alpha-adrenergique dans le cornet nasal humain] Can J Anesth, July 1, 2007; 54(7): 549 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Michelotti, D. M. Brinkley, D. P. Morris, M. P. Smith, R. J. Louie, and D. A. Schwinn Epigenetic regulation of human {alpha}1d-adrenergic receptor gene expression: a role for DNA methylation in Sp1-dependent regulation FASEB J, July 1, 2007; 21(9): 1979 - 1993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Huang, L.L. Wu, Y.Y. Zhang, Y. Gao, and G.Y. Yu Functional {alpha}1-Adrenoceptor Subtypes in Human Submandibular Glands. J. Dent. Res., March 1, 2006; 85(3): 251 - 256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Israilova, T. Tanaka, F. Suzuki, S. Morishima, and I. Muramatsu Pharmacological Characterization and Cross Talk of {alpha}1A- and {alpha}1B-Adrenoceptors Coexpressed in Human Embryonic Kidney 293 Cells J. Pharmacol. Exp. Ther., April 1, 2004; 309(1): 259 - 266. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. A. Uberti, R. A. Hall, and K. P. Minneman Subtype-Specific Dimerization of {alpha}1-Adrenoceptors: Effects on Receptor Expression and Pharmacological Properties Mol. Pharmacol., December 1, 2003; 64(6): 1379 - 1390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Michelotti, M. J. Bauman, M. P. Smith, and D. A. Schwinn Cloning and Characterization of the Rat alpha 1a-Adrenergic Receptor Gene Promoter. DEMONSTRATION OF CELL SPECIFICITY AND REGULATION BY HYPOXIA J. Biol. Chem., February 28, 2003; 278(10): 8693 - 8705. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Rokosh and P. C. Simpson Knockout of the alpha 1A/C-adrenergic receptor subtype: The alpha 1A/C is expressed in resistance arteries and is required to maintain arterial blood pressure PNAS, July 9, 2002; 99(14): 9474 - 9479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kawanabe, N. Hashimoto, T. Masaki, and S. Miwa Ca2+ Influx through Nonselective Cation Channels Plays an Essential Role in Noradrenaline-Induced Arachidonic Acid Release in Chinese Hamster Ovary Cells Expressing alpha 1A-, alpha 1B-, or alpha 1D-Adrenergic Receptors J. Pharmacol. Exp. Ther., December 1, 2001; 299(3): 901 - 907. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Salvi Protecting the Myocardium From Ischemic Injury : A Critical Role for {{alpha}}1-Adrenoreceptors? Chest, April 1, 2001; 119(4): 1242 - 1249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Shapiro, B. Winters, M. Hales, T. Barnett, D. A. Schwinn, N. Flavahan, and D. E. Berkowitz Endogenous Circulating Sympatholytic Factor in Orthostatic Intolerance Hypertension, October 1, 2000; 36(4): 553 - 560. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. L. Rudner, D. E. Berkowitz, J. V. Booth, B. L. Funk, K. L. Cozart, E. B. D’Amico, H. El-Moalem, S. O. Page, C. D. Richardson, B. Winters, et al. Subtype Specific Regulation of Human Vascular {alpha}1-Adrenergic Receptors by Vessel Bed and Age Circulation, December 7, 1999; 100(23): 2336 - 2343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O.-E. Brodde and M. C. Michel Adrenergic and Muscarinic Receptors in the Human Heart Pharmacol. Rev., December 1, 1999; 51(4): 651 - 690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-Y. Zhang, K.-M. Xu, and C. Han alpha 1-Adrenoceptor Subtypes Mediating Inotropic Responses in Rat Heart J. Pharmacol. Exp. Ther., November 1, 1999; 291(2): 829 - 836. [Abstract] [Full Text]
|
|
|
|
|
|
Z.-W. Hu, X.-Y. Shi, R. Z. Lin, J. Chen, and B. B. Hoffman alpha 1-Adrenergic Receptor Stimulation of Mitogenesis in Human Vascular Smooth Muscle Cells: Role of Tyrosine Protein Kinases and Calcium in Activation of Mitogen-Activated Protein Kinase J. Pharmacol. Exp. Ther., July 1, 1999; 290(1): 28 - 37. [Abstract] [Full Text]
|
|
|
|
|
|
S. L. Hrometz, S. E. Edelmann, D. F. McCune, J. R. Olges, R. W. Hadley, D. M. Perez, and M. T. Piascik Expression of Multiple alpha 1-Adrenoceptors on Vascular Smooth Muscle: Correlation with the Regulation of Contraction J. Pharmacol. Exp. Ther., July 1, 1999; 290(1): 452 - 463. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. AVKIRAN and R. S. HAWORTH Regulation of Cardiac Sarcolemmal Na Ann. N.Y. Acad. Sci., June 30, 1999; 874(1): 335 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Berkowitz, D. Brown, K. M. Lee, C. Emala, D. Palmer, Y. An, and M. Breslow Endotoxin-induced alteration in the expression of leptin and beta 3-adrenergic receptor in adipose tissue Am J Physiol Endocrinol Metab, June 1, 1998; 274(6): E992 - E997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yokoyama, M. Yasutake, and M. Avkiran {alpha}1-Adrenergic Stimulation of Sarcolemmal Na+-H+ Exchanger Activity in Rat Ventricular Myocytes : Evidence for Selective Mediation by the {alpha}1A-Adrenoceptor Subtype Circ. Res., June 1, 1998; 82(10): 1078 - 1085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Razik, K. Lee, R. R. Price, M. R. Williams, R. R. Ongjoco, M. K. Dole, X. L. Rudner, M. M. Kwatra, and D. A. Schwinn Transcriptional Regulation of the Human alpha 1a-Adrenergic Receptor Gene. CHARACTERIZATION OF THE 5'-REGULATORY AND PROMOTER REGION J. Biol. Chem., November 7, 1997; 272(45): 28237 - 28246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Graham, D. M. Perez, J. Hwa, and M. T. Piascik {alpha}1-Adrenergic Receptor Subtypes : Molecular Structure, Function, and Signaling Circ. Res., May 1, 1996; 78(5): 737 - 749. [Full Text]
|
|
 |
 |
 |
|
 |
 |
 |
