![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
G Nickenig and TJ Murphy
Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia 30322.
The effects of epidermal growth factor, basic fibroblast growth factor, and platelet-derived growth factor-BB on angiotensin type 1 (AT1) receptor gene expression were examined in rat thoracic aorta vascular smooth muscle cells (VSMC) in culture. Incubation of serum-deprived VSMC with 20 ng/ml epidermal growth factor, 20 ng/ml basic fibroblast growth factor, or 50 ng/ml platelet-derived growth factor-BB reduces AT1 receptor mRNA levels, as assessed by Northern hybridization analysis, to approximately 30% of control levels. This effect is maximal 4 hr after addition of each growth factor to the culture medium and is sustained for up to 24 hr of incubation after a single dose. There is a correlative loss of membrane-associated AT1 receptors and angiotensin II-stimulated inositol phosphate production after 24 hr of growth factor treatment. The half-life of AT1 receptor mRNA is reduced significantly by growth factors, compared with that for cells treated with actinomycin D alone to block transcription. This suggests that growth factors activate a mechanism that involves post-transcriptional destabilization of AT1 receptor mRNA. This effect can be blocked by prior treatment of VSMC with actinomycin D or cycloheximide, suggesting that the effect of the growth factors on AT1 receptor gene expression is mediated through induction of an unknown gene or genes that function to destabilize AT1 receptor mRNA and that mRNA translation is essential for the destabilizing effect. Nuclear run-on assays reveal that the growth factors also significantly reduce the rate of de novo AT1 receptor gene transcription. Thus, down-regulation of AT1 receptor gene expression by growth factors also appears to involve mechanisms that decrease the rate of AT1 receptor gene transcription. These data reveal marked down-regulation of AT1 receptor gene expression in VSMC by growth factor receptor activation, through mechanisms that involve both attenuation of transcription and post-transcriptional mRNA destabilization.
This article has been cited by other articles:
|
|
 |
|
  K. Paukku, N. Kalkkinen, O. Silvennoinen, K. K. Kontula, and J. Y. A. Lehtonen p100 increases AT1R expression through interaction with AT1R 3'-UTR Nucleic Acids Res., August 1, 2008; 36(13): 4474 - 4487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. S. Elton and M. M. Martin Angiotensin II Type 1 Receptor Gene Regulation: Transcriptional and Posttranscriptional Mechanisms Hypertension, May 1, 2007; 49(5): 953 - 961. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lee, H. Ji, Z. Wu, W. Zheng, A. Hassan, and K. Sandberg Translational regulation of ANG II type 1 receptors in proliferating vascular smooth muscle cells Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2006; 290(1): R50 - R56. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Olivares-Reyes, B. H. Shah, J. Hernandez-Aranda, A. Garcia-Caballero, M. P. Farshori, J. A. Garcia-Sainz, and K. J. Catt Agonist-Induced Interactions between Angiotensin AT1 and Epidermal Growth Factor Receptors Mol. Pharmacol., August 1, 2005; 68(2): 356 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wassmann, M. Stumpf, K. Strehlow, A. Schmid, B. Schieffer, M. Bohm, and G. Nickenig Interleukin-6 Induces Oxidative Stress and Endothelial Dysfunction by Overexpression of the Angiotensin II Type 1 Receptor Circ. Res., March 5, 2004; 94(4): 534 - 541. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Y. Park, C. Y. Song, B. C. Kim, H. K. Hong, and H. S. Lee Angiotensin II mediates LDL-induced superoxide generation in mesangial cells Am J Physiol Renal Physiol, November 1, 2003; 285(5): F909 - F915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-K. Yeh, T. K. Hymer, A. L. Sousa, B.-X. Zhang, M. D. Lifschitz, and M. S. Katz Epidermal growth factor upregulates beta -adrenergic receptor signaling in a human salivary cell line Am J Physiol Cell Physiol, May 1, 2003; 284(5): C1164 - C1175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Nickenig and D. G. Harrison The AT1-Type Angiotensin Receptor in Oxidative Stress and Atherogenesis: Part II: AT1 Receptor Regulation Circulation, January 29, 2002; 105(4): 530 - 536. [Full Text] [PDF]
|
|
|
|
|
|
S. Wassmann, U. Laufs, A. T. Bäumer, K. Müller, C. Konkol, H. Sauer, M. Böhm, and G. Nickenig Inhibition of Geranylgeranylation Reduces Angiotensin II-Mediated Free Radical Production in Vascular Smooth Muscle Cells: Involvement of Angiotensin AT1 Receptor Expression and Rac1 GTPase Mol. Pharmacol., March 1, 2001; 59(3): 646 - 654. [Abstract] [Full Text]
|
|
|
|
|
|
T. Ichiki, K. Takeda, T. Tokunou, Y. Funakoshi, K. Ito, N. Iino, and A. Takeshita Reactive Oxygen Species-Mediated Homologous Downregulation of Angiotensin II Type 1 Receptor mRNA by Angiotensin II Hypertension, February 1, 2001; 37(2): 535 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wassmann, A. T. Baumer, K. Strehlow, M. van Eickels, C. Grohe, K. Ahlbory, R. Rosen, M. Bohm, and G. Nickenig Endothelial Dysfunction and Oxidative Stress During Estrogen Deficiency in Spontaneously Hypertensive Rats Circulation, January 23, 2001; 103(3): 435 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Nickenig, K. Strehlow, S. Wassmann, A. T. Baumer, K. Albory, H. Sauer, and M. Bohm Differential Effects of Estrogen and Progesterone on AT1 Receptor Gene Expression in Vascular Smooth Muscle Cells Circulation, October 10, 2000; 102(15): 1828 - 1833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Robida, K. Xu, M. L. Ellington, and T. J. Murphy Cyclosporin A Selectively Inhibits Mitogen-Induced Cyclooxygenase-2 Gene Transcription in Vascular Smooth Muscle Cells Mol. Pharmacol., October 1, 2000; 58(4): 701 - 708. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Muller, A. Reddert, S. Wassmann, K. Strehlow, M. Bohm, and G. Nickenig Insulin-like growth factor induces up-regulation of AT1-receptor gene expression in vascular smooth muscle cells Journal of Renin-Angiotensin-Aldosterone System, September 1, 2000; 1(3): 273 - 277. [Abstract] [PDF]
|
|
|
|
 |
|
 K. Xu and T. J. Murphy Reconstitution of Angiotensin Receptor mRNA Down-regulation in Vascular Smooth Muscle. POST-TRANSCRIPTIONAL CONTROL BY PROTEIN KINASE A BUT NOT MITOGENIC SIGNALING DIRECTED BY THE 5'-UNTRANSLATED REGION J. Biol. Chem., March 10, 2000; 275(11): 7604 - 7611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Xin, N. Yang, and J. E. Faber Platelet-Derived Growth Factor Inhibits alpha 1D-Adrenergic Receptor Expression in Vascular Smooth Muscle Cells In Vitro and Ex Vivo Mol. Pharmacol., December 1, 1999; 56(6): 1143 - 1151. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Q. Liu Focal Expression of Angiotensin II Type 1 Receptor and Smooth Muscle Cell Proliferation in the Neointima of Experimental Vein Grafts : Relation to Eddy Blood Flow Arterioscler. Thromb. Vasc. Biol., November 1, 1999; 19(11): 2630 - 2639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Strehlow, G. Nickenig, J. Roeling, S. Wassmann, O. Zolk, A. Knorr, and M. Bohm AT1 receptor regulation in salt-sensitive hypertension Am J Physiol Heart Circ Physiol, November 1, 1999; 277(5): H1701 - H1707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Gurantz, R. T. Cowling, F. J. Villarreal, and B. H. Greenberg Tumor Necrosis Factor-{alpha} Upregulates Angiotensin II Type 1 Receptors on Cardiac Fibroblasts Circ. Res., August 6, 1999; 85(3): 272 - 279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Adams, T. S. Obertone, X. Wang, and T. J. Murphy Relationship between Internalization and mRNA Decay in Down-Regulation of Recombinant Type 1 Angiotensin II Receptor (AT1) Expression in Smooth Muscle Cells Mol. Pharmacol., June 1, 1999; 55(6): 1028 - 1036. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Tazawa, T. Nakane, and S. Chiba Angiotensin II Type 1 Receptor Blockade Prevents Up-Regulation of Angiotensin II Type 1A Receptors in Rat Injured Artery J. Pharmacol. Exp. Ther., February 1, 1999; 288(2): 898 - 904. [Abstract] [Full Text]
|
|
|
|
|
|
G. Nickenig, J. Roling, K. Strehlow, P. Schnabel, and M. Bohm Insulin Induces Upregulation of Vascular AT1 Receptor Gene Expression by Posttranscriptional Mechanisms Circulation, December 1, 1998; 98(22): 2453 - 2460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wang and T. J. Murphy Inhibition of Cyclic AMP-Dependent Kinase by Expression of a Protein Kinase Inhibitor/Enhanced Green Fluorescent Fusion Protein Attenuates Angiotensin II-Induced Type 1 AT1 Receptor mRNA Down-Regulation in Vascular Smooth Muscle Cells Mol. Pharmacol., September 1, 1998; 54(3): 514 - 524. [Abstract] [Full Text]
|
|
|
|
|
|
G. Nickenig, A. T. Baumer, C. Grohe, S. Kahlert, K. Strehlow, S. Rosenkranz, A. Stablein, F. Beckers, J. F. M. Smits, M. J. A. P. Daemen, et al. Estrogen Modulates AT1 Receptor Gene Expression In Vitro and In Vivo Circulation, June 9, 1998; 97(22): 2197 - 2201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Nickenig, K. Strehlow, J. Roeling, O. Zolk, A. Knorr, and M. Bohm Salt Induces Vascular AT1 Receptor Overexpression In Vitro and In Vivo Hypertension, June 1, 1998; 31(6): 1272 - 1277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wang, G. Nickenig, and T. J. Murphy The Vascular Smooth Muscle Type I Angiotensin II Receptor mRNA Is Destabilized by Cyclic AMP-Elevating Agents Mol. Pharmacol., November 1, 1997; 52(5): 781 - 787. [Abstract] [Full Text]
|
|
|
|
|
|
M. E. Ullian, J. R. Raymond, M. C. Willingham, and R. V. Paul Regulation of vascular angiotensin II receptors by EGF Am J Physiol Cell Physiol, October 1, 1997; 273(4): C1241 - C1249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Clements and J. E. Faber Mechanical Load Opposes Angiotensin-Mediated Decrease in Vascular {alpha}1-Adrenoceptors Hypertension, May 1, 1997; 29(5): 1165 - 1172. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Ouali, M.-C. Berthelon, M. Begeot, and J. M. Saez Angiotensin II Receptor Subtypes AT1 and AT2 Are Down-Regulated by Angiotensin II through AT1 Receptor by Different Mechanisms Endocrinology, February 1, 1997; 138(2): 725 - 733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Nickenig, A. Sachinidis, F. Michaelsen, M. Bohm, S. Seewald, and H. Vetter Upregulation of Vascular Angiotensin II Receptor Gene Expression by Low-Density Lipoprotein in Vascular Smooth Muscle Cells Circulation, January 21, 1997; 95(2): 473 - 478. [Abstract] [Full Text]
|
|
|
|
|
|
K. Kijima, H. Matsubara, S. Murasawa, K. Maruyama, Y. Mori, N. Ohkubo, I. Komuro, Y. Yazaki, T. Iwasaka, and M. Inada Mechanical Stretch Induces Enhanced Expression of Angiotensin II Receptor Subtypes in Neonatal Rat Cardiac Myocytes Circ. Res., October 1, 1996; 79(4): 887 - 897. [Abstract] [Full Text]
|
|
|
|
|
|
B. S. Dixon, R. V. Sharma, and M. J. Dennis The Bradykinin B2 Receptor Is a Delayed Early Response Gene for Platelet-derived Growth Factor in Arterial Smooth Muscle Cells J. Biol. Chem., June 7, 1996; 271(23): 13324 - 13332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Xu, A. M. Robida, and T. J. Murphy Immediate-early MEK-1-dependent Stabilization of Rat Smooth Muscle Cell Cyclooxygenase-2 mRNA by Galpha q-coupled Receptor Signaling J. Biol. Chem., July 21, 2000; 275(30): 23012 - 23019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Nickenig, F. Michaelsen, C. Muller, A. Berger, T. Vogel, A. Sachinidis, H. Vetter, and M. Bohm Destabilization of AT1 Receptor mRNA by Calreticulin Circ. Res., January 11, 2002; 90(1): 53 - 58. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
