![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
AB Bloomenthal, E Goldwater, DB Pritchett and NL Harrison
Department of Anesthesia and Critical Care, University of Chicago, Illinois 60637.
The effects of extracellular applications of Zn2+ ions on the strychnine-sensitive glycine receptor were studied in cultured rat spinal cord neurons and with recombinant glycine receptors expressed in human embryonic kidney 293 cells. Nanomolar concentrations of Zn2+ enhanced the chloride ion current in response to brief applications of 100 microM glycine. The enhancement of glycine responses increased from 20 nM to 1 microM Zn2+. Higher concentrations of Zn2+ caused a reversal of the potentiation, followed by progressive inhibition of the glycine response up to approximately 20-50 microM Zn2+. The biphasic modulation by Zn2+ appeared essentially identical in native and recombinant glycine receptors. Biphasic Zn2+ modulation was observed both with picrotoxin-insensitive heteromeric (alpha 2/beta) receptors and with picrotoxin-sensitive homomeric receptors consisting only of alpha 2 subunits. This suggests that the alpha subunit alone is sufficient for formation of two distinct Zn2+ binding sites on the glycine receptor. The demonstration of Zn2+ modulation of the strychnine-sensitive glycine receptor is of potential physiological importance, in view of the likely range of subsynaptic Zn2+ concentrations to which the receptor is exposed.
This article has been cited by other articles:
|
|
 |
|
  D. D. Mott, M. Benveniste, and R. J. Dingledine pH-Dependent Inhibition of Kainate Receptors by Zinc J. Neurosci., February 13, 2008; 28(7): 1659 - 1671. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Hsiao, K. B. Mihalak, K. L. Magleby, and C. W. Luetje Zinc Potentiates Neuronal Nicotinic Receptors by Increasing Burst Duration J Neurophysiol, February 1, 2008; 99(2): 999 - 1007. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Coddou, C. Acuna-Castillo, P. Bull, and J. P. Huidobro-Toro Dissecting the Facilitator and Inhibitor Allosteric Metal Sites of the P2X4 Receptor Channel: CRITICAL ROLES OF CYS132 FOR ZINC POTENTIATION AND ASP138 FOR COPPER INHIBITION J. Biol. Chem., December 21, 2007; 282(51): 36879 - 36886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. X. Zhang and L. L. Thio Zinc Enhances the Inhibitory Effects of Strychnine-Sensitive Glycine Receptors in Mouse Hippocampal Neurons J Neurophysiol, December 1, 2007; 98(6): 3666 - 3676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Miller, H. M. A. Da Silva, and T. G. Smart Molecular Basis for Zinc Potentiation at Strychnine-sensitive Glycine Receptors J. Biol. Chem., November 11, 2005; 280(45): 37877 - 37884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. S Miller, M. Beato, R. J Harvey, and T. G Smart Molecular determinants of glycine receptor {alpha}{beta} subunit sensitivities to Zn2+-mediated inhibition J. Physiol., August 1, 2005; 566(3): 657 - 670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. G. Smart, A. M. Hosie, and P. S. Miller Zn2+ Ions: Modulators of Excitatory and Inhibitory Synaptic Activity Neuroscientist, October 1, 2004; 10(5): 432 - 442. [Abstract] [PDF]
|
|
|
|
 |
|
 J. W. Lynch Molecular Structure and Function of the Glycine Receptor Chloride Channel Physiol Rev, October 1, 2004; 84(4): 1051 - 1095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gruss, A. Mathie, W. R. Lieb, and N. P. Franks The Two-Pore-Domain K+ Channels TREK-1 and TASK-3 Are Differentially Modulated by Copper and Zinc Mol. Pharmacol., September 1, 2004; 66(3): 530 - 537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ruiz, M. C. Walker, R. Fabian-Fine, and D. M. Kullmann Endogenous Zinc Inhibits GABAA Receptors in a Hippocampal Pathway J Neurophysiol, February 1, 2004; 91(2): 1091 - 1096. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Coddou, B. Morales, J. Gonzalez, M. Grauso, F. Gordillo, P. Bull, F. Rassendren, and J. P. Huidobro-Toro Histidine 140 Plays a Key Role in the Inhibitory Modulation of the P2X4 Nucleotide Receptor by Copper but Not Zinc J. Biol. Chem., September 19, 2003; 278(38): 36777 - 36785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Thio, A. Shanmugam, K. Isenberg, and K. Yamada Benzodiazepines Block {alpha}2-Containing Inhibitory Glycine Receptors in Embryonic Mouse Hippocampal Neurons J Neurophysiol, July 1, 2003; 90(1): 89 - 99. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Davies, W. Wang, T. G. Hales, and E. F. Kirkness A Novel Class of Ligand-gated Ion Channel Is Activated by Zn2+ J. Biol. Chem., January 3, 2003; 278(2): 712 - 717. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Martin and G. R. Siggins Electrophysiological Evidence for Expression of Glycine Receptors in Freshly Isolated Neurons from Nucleus Accumbens J. Pharmacol. Exp. Ther., September 1, 2002; 302(3): 1135 - 1145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Jentsch, V. Stein, F. Weinreich, and A. A. Zdebik Molecular Structure and Physiological Function of Chloride Channels Physiol Rev, April 1, 2002; 82(2): 503 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Chattipakorn and L. L. McMahon Pharmacological Characterization of Glycine-Gated Chloride Currents Recorded in Rat Hippocampal Slices J Neurophysiol, March 1, 2002; 87(3): 1515 - 1525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Hsiao, D. Dweck, and C. W. Luetje Subunit-Dependent Modulation of Neuronal Nicotinic Receptors by Zinc J. Neurosci., March 15, 2001; 21(6): 1848 - 1856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Suwa, L. Saint-Amant, A. Triller, P. Drapeau, and P. Legendre High-Affinity Zinc Potentiation of Inhibitory Postsynaptic Glycinergic Currents in the Zebrafish Hindbrain J Neurophysiol, February 1, 2001; 85(2): 912 - 925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Loland, L. Norregaard, and U. Gether Defining Proximity Relationships in the Tertiary Structure of the Dopamine Transporter. IDENTIFICATION OF A CONSERVED GLUTAMIC ACID AS A THIRD COORDINATE IN THE ENDOGENOUS Zn2+-BINDING SITE J. Biol. Chem., December 24, 1999; 274(52): 36928 - 36934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. BETZ, J. KUHSE, V. SCHMIEDEN, B. LAUBE, J. KIRSCH, and R. J. HARVEY Structure and Functions of Inhibitory and Excitatory Glycine Receptors Ann. N.Y. Acad. Sci., April 30, 1999; 868(1): 667 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Han and S. M. Wu Modulation of glycine receptors in retinal ganglion cells by zinc PNAS, March 16, 1999; 96(6): 3234 - 3238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Q. Trombley Selective Modulation of GABAA Receptors by Aluminum J Neurophysiol, August 1, 1998; 80(2): 755 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wakamori, M. Strobeck, T. Niidome, T. Teramoto, K. Imoto, and Y. Mori Functional Characterization of Ion Permeation Pathway in the N-Type Ca2+ Channel J Neurophysiol, February 1, 1998; 79(2): 622 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Shan, J. L. Haddrill, and J. W. Lynch Ivermectin, an Unconventional Agonist of the Glycine Receptor Chloride Channel J. Biol. Chem., April 13, 2001; 276(16): 12556 - 12564. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
