![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
F Larini, P Menegazzi, O Baricordi, F Zorzato and S Treves
Institute of General Pathology, University of Ferrara, Italy.
In this study we investigated the effects of 4-chloro-3-ethylphenol on Ca2+ homeostasis, and we report that this compound induces Ca2+ release from a ruthenium red-sensitive Ca2+ release channel present in skeletal muscle terminal cisternae. When tested with bovine cerebellar microsomes, the Ca(2+)-releasing activity of 4-chloro-3-ethylphenol was reduced by ruthenium red and unaffected by heparin. In PC-12 cells, HL- 60 cells, human fibroblasts, the human hepatoma cell line PLC/PRF/5, and Jurkat cells, 4-chloro-3-ethylphenol released Ca2+ from intracellular thapsigargin-sensitive stores. Although decreased, its effect was retained after treatment of intact/permeabilized cells with inositol-1,4,5-trisphosphate (IP3)-mobilizing agonists/IP3, whereas pretreatment of permeabilized cells with ruthenium red reduced the Ca(2+)-releasing activity of 4-chloro-3-ethylphenol. These results provide functional evidence for the presence of a Ca2+ channel distinct from the IP3 receptor, having pharmacological similarities to the ryanodine receptor, in the intracellular Ca2+ stores of a variety of nonexcitable cells. We also demonstrate that a monoclonal anti- ryanodine receptor antibody recognizes a protein in human fibroblasts with similar apparent molecular mass the ryanodine receptor. Thus, the intracellular Ca2+ stores of mammalian cells appear to be endowed with two distinct intracellular Ca2+ channels.
This article has been cited by other articles:
|
|
 |
|
  T. Tazzeo, Y. Zhang, S. Keshavjee, and L. J. Janssen Ryanodine receptors decant internal Ca2+ store in human and bovine airway smooth muscle Eur. Respir. J., August 1, 2008; 32(2): 275 - 284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Jacobson, S. T. Moe, P. D. Allen, and J. D. Fessenden Structural Determinants of 4-Chloro-m-cresol Required for Activation of Ryanodine Receptor Type 1 Mol. Pharmacol., July 1, 2006; 70(1): 259 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. U. Gerbershagen, M. Fiege, R. Weisshorn, K. Kolodzie, J. S. a. Esch, and F. Wappler Cumulative and Bolus In Vitro Contracture Testing with 4-Chloro-3-Ethylphenol in Malignant Hyperthermia Positive and Negative Human Skeletal Muscles Anesth. Analg., September 1, 2005; 101(3): 710 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Laporte, A. Hui, and I. Laher Pharmacological Modulation of Sarcoplasmic Reticulum Function in Smooth Muscle Pharmacol. Rev., December 1, 2004; 56(4): 439 - 513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. H. Wong, J. D. Johnson, W. K. Yunker, and J. P. Chang Caffeine stores and dopamine differentially require Ca2+ channels in goldfish somatotropes Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2001; 280(2): R494 - R503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zemkova and J. VanecEK Differences in Gonadotropin-Releasing Hormone-Induced Calcium Signaling between Melatonin-Sensitive and Melatonin-Insensitive Neonatal Rat Gonadotrophs Endocrinology, March 1, 2000; 141(3): 1017 - 1026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. DiJulio, E. L. Watson, I. N. Pessah, K. L. Jacobson, S. M. Ott, E. D. Buck, and J. C. Singh Ryanodine Receptor Type III (Ry3R) Identification In Mouse Parotid Acini. PROPERTIES AND MODULATION OF [3H]RYANODINE-BINDING SITES J. Biol. Chem., June 20, 1997; 272(25): 15687 - 15696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zucchi and S. Ronca-Testoni The Sarcoplasmic Reticulum Ca2+ Channel/Ryanodine Receptor: Modulation by Endogenous Effectors, Drugs and Disease States Pharmacol. Rev., March 1, 1997; 49(1): 1 - 52. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
