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Molecular Pharmacology, Vol 5, 26-29, Copyright © 1969 by the American Society for Pharmacology and Experimental Therapeutics

Inosinic Acid Dehydrogenase in Mammalian Tissues

P. A. SACCOCCIA JR. 1 and R. P. MIECH 1

1 Division of Biological and Medical Sciences, Brown University, Providence, Rhode Island 02912

The enzyme inosinic acid dehydrogenase (IMP:NAD oxidoreductase, EC 1.2.1.14), which catalyzes the reaction IMP + NAD+ + H2O [unknown] XMP + NADH + [H+ has been demonstrated and quantitatively determined for the first time in crude extracts of spleen, lung, and brain. This was achieved by means of a new radiochemical assay for this enzyme. Reasons for the previous inability to measure this enzyme in extracts of mammalian tissues with spectrophotometric techniques are discussed. Potential uses of this new IMP dehydrogenase assay in investigating the mechanism of action of several cytolytic purine analogues in vivo are suggested.

Note:
ACKNOWLEDGMENTS The authors are indebted to Miss Janice Santos and Mrs. Dalia Rosen for their skillful technical assistance.

Submitted on August 12, 1968






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