![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Department of Physiology and Biophysics, School of Medicine, Case
Western Reserve University, Cleveland, Ohio 44106-4970
HL-60 human promyelocytic leukocytes express G protein-coupled
P2U-purinergic nucleotide receptors (P2UR or
P2Y2R) that activate inositol phospholipid hydrolysis and
Ca2+ mobilization in response to ATP or UTP. We examined
the expression of functional P2UR and P2UR mRNA
levels during in vitro differentiation of HL-60 cells by
dibutyryl-cAMP (Bt2cAMP), which induces a
granulocyte/neutrophil phenotype, or by phorbol-12-myristate-13-acetate
(PMA), which induces a monocyte/macrophage phenotype. Both
P2UR function and P2UR mRNA levels were only
modestly attenuated during granulocytic differentiation by
Bt2cAMP. In contrast, P2UR function, as assayed by either Ca2+ mobilization or inositol trisphosphate
generation, was greatly reduced in PMA-differentiated cells. This
inhibition of P2UR function was strongly correlated with
PMA-induced decreases in P2UR mRNA levels, as assayed by
Northern blot analysis or reverse transcription-polymerase chain
reaction-based quantification. Although PMA induced an early, transient
up-regulation of P2UR mRNA, this was rapidly followed by a
sustained decrease in P2UR mRNA to a level 5-10-fold lower than that in undifferentiated HL-60 cells. The half-life of the P2UR transcript in HL-60 cells was ~60 min, and this was
not affected by acute exposure (
4 hr) to Bt2cAMP or PMA.
PMA down-regulated P2UR mRNA in THP-1 monocytes and HL-60
granulocytes but not in A431 human epithelial cells or human
keratinocytes. P2UR mRNA was also down-regulated in THP-1
monocytes differentiated into inflammatory macrophages by
-interferon and endotoxin. These data indicate that myeloid
leukocytes possess tissue-specific mechanisms for the rapid modulation
of P2UR expression and function during differentiation and
inflammatory activation.
This article has been cited by other articles:
|
|
 |
|
  M. P. Abbracchio, G. Burnstock, J.-M. Boeynaems, E. A. Barnard, J. L. Boyer, C. Kennedy, G. E. Knight, M. Fumagalli, C. Gachet, K. A. Jacobson, et al. International Union of Pharmacology LVIII: Update on the P2Y G Protein-Coupled Nucleotide Receptors: From Molecular Mechanisms and Pathophysiology to Therapy Pharmacol. Rev., September 1, 2006; 58(3): 281 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Meshki, F. Tuluc, O. Bredetean, Z. Ding, and S. P. Kunapuli Molecular mechanism of nucleotide-induced primary granule release in human neutrophils: role for the P2Y2 receptor Am J Physiol Cell Physiol, February 1, 2004; 286(2): C264 - C271. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Aga, C. J. Johnson, A. P. Hart, A. G. Guadarrama, M. Suresh, J. Svaren, P. J. Bertics, and B. J. Darien Modulation of monocyte signaling and pore formation in response to agonists of the nucleotide receptor P2X7 J. Leukoc. Biol., July 1, 2002; 72(1): 222 - 232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.-C. Suh, J.-S. Kim, U. Namgung, H. Ha, and K.-T. Kim P2X7 Nucleotide Receptor Mediation of Membrane Pore Formation and Superoxide Generation in Human Promyelocytes and Neutrophils J. Immunol., June 1, 2001; 166(11): 6754 - 6763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Stober, D. A. Lammas, C. M. Li, D. S. Kumararatne, S. L. Lightman, and C. A. McArdle ATP-Mediated Killing of Mycobacterium bovis Bacille Calmette-Guerin Within Human Macrophages Is Calcium Dependent and Associated with the Acidification of Mycobacteria-Containing Phagosomes J. Immunol., May 15, 2001; 166(10): 6276 - 6286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Erb, J. Liu, J. Ockerhausen, Q. Kong, R. C. Garrad, K. Griffin, C. Neal, B. Krugh, L. I. Santiago-Perez, F. A. Gonzalez, et al. An RGD Sequence in the P2Y2 Receptor Interacts with {alpha}V{beta}3 Integrins and Is Required for Go-mediated Signal Transduction J. Cell Biol., April 24, 2001; 153(3): 491 - 502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Di Virgilio, P. Chiozzi, D. Ferrari, S. Falzoni, J. M. Sanz, A. Morelli, M. Torboli, G. Bolognesi, and O. R. Baricordi Nucleotide receptors: an emerging family of regulatory molecules in blood cells Blood, February 1, 2001; 97(3): 587 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hou, S. Moller, L. Edvinsson, and D. Erlinge Cytokines Induce Upregulation of Vascular P2Y2 Receptors and Increased Mitogenic Responses to UTP and ATP Arterioscler. Thromb. Vasc. Biol., September 1, 2000; 20(9): 2064 - 2069. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. John, E. Scemes, S. O. Suadicani, J. S. H. Liu, P. C. Charles, S. C. Lee, D. C. Spray, and C. F. Brosnan IL-1beta differentially regulates calcium wave propagation between primary human fetal astrocytes via pathways involving P2 receptors and gap junction channels PNAS, September 28, 1999; 96(20): 11613 - 11618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R. Woodman, J. M. Muller, J. W. E. Rush, M. H. Laughlin, and E. M. Price Flow regulation of ecNOS and Cu/Zn SOD mRNA expression in porcine coronary arterioles Am J Physiol Heart Circ Physiol, March 1, 1999; 276(3): H1058 - H1063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hu, P. L. Fisette, L. C. Denlinger, A. G. Guadarrama, J. A. Sommer, R. A. Proctor, and P. J. Bertics Purinergic Receptor Modulation of Lipopolysaccharide Signaling and Inducible Nitric-oxide Synthase Expression in RAW 264.7 Macrophages J. Biol. Chem., October 16, 1998; 273(42): 27170 - 27175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Ralevic and G. Burnstock Receptors for Purines and Pyrimidines Pharmacol. Rev., September 1, 1998; 50(3): 413 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. I. Gorodeski, P. Burfeind, S. U. Gan, D. Pal, and F. W. Abdul-Karim Regulation by retinoids of P2Y2 nucleotide receptor mRNA in human uterine cervical cells Am J Physiol Cell Physiol, September 1, 1998; 275(3): C758 - C765. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Li, V. A. Vaidya, J. D. Alvaro, P. A. Iredale, R. Hsu, G. Hoffman, L. Fitzgerald, P. K. Curran, C. A. Machida, P. H. Fishman, et al. Protein Kinase C-Mediated Down-Regulation of beta 1-Adrenergic Receptor Gene Expression in Rat C6 Glioma Cells Mol. Pharmacol., July 1, 1998; 54(1): 14 - 21. [Abstract] [Full Text]
|
|
|
|
|
|
C. R. Woodman, J. M. Muller, M. H. Laughlin, and E. M. Price Induction of nitric oxide synthase mRNA in coronary resistance arteries isolated from exercise-trained pigs Am J Physiol Heart Circ Physiol, December 1, 1997; 273(6): H2575 - H2579. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
