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0026-895X/97/020293-08$3.00/0
Copyright © by The American Society for Pharmacology and Experimental Therapeutics
All rights of reproduction in any form reserved.
MOLECULAR PHARMACOLOGY 51:293-300 (1997).

Molecular Cloning and Pharmacological Characterization of a Molluscan Octopamine Receptor

Cindy C. Gerhardt, Remko A. Bakker, Gerard J. Piek, Rudi J. Planta, Erno Vreugdenhil,1 Josée E. Leysen, and Harm Van Heerikhuizen

Department of Biochemistry and Molecular Biology, Research Institute Neurosciences, Vrije Universiteit, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands (C.C.G., R.A.B., G.J.P., R.J.P., E.V., H.v.H.), Department of Biochemical Pharmacology, Janssen Research Foundation, Turnhoutseweg 30, B2340 Beerse, Belgium (J.E.L.), and Department of Pharmacology, Vrije Universiteit, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands (J.E.L.)

We describe the cloning and functional expression of a cDNA encoding a novel G protein-coupled receptor, which was isolated from the central nervous system of the pond snail Lymnaea stagnalis. The amino acid sequence predicted by this cDNA shows highest similarity with the sequence of the Locusta tyramine receptor, the Drosophila tyramine/octopamine receptor, and the mammalian alpha -adrenergic receptors. On expression in mammalian cells, [3H]rauwolscine, an alpha 2-adrenergic receptor antagonist, binds with high affinity (KD = 2.9 × 10-9 M) to the receptor. Of several tested neurotransmitters, octopamine (which is considered to be the invertebrate counterpart of norepinephrine) showed the highest affinity (1.9 × 10-6 M) for the receptor. Therefore, we consider this receptor to be the first true octopamine receptor to be cloned. The ligand binding properties of the novel receptor, designated Lym oa1, seem to be distinct from any of the binding profiles described for octopamine receptors in tissue preparations. Although the pharmacological profile of Lym oa1 shows some similarity with that of Tyr/Oct-Dro and Tyr-Loc, there are also clear differences. In particular, phentolamine, chlorpromazine, and mianserine display markedly higher affinities for Lym oa1 than for the insect receptors. As far as the vertebrate adrenergic receptors are concerned, the ligand binding properties of Lym oa1 resemble alpha 2-adrenergic receptors more than they do alpha 1- or beta -adrenergic receptors. Octopaminergic stimulation of Lym oa1 induces an increase in both inositol phosphates and cAMP (EC50 = 9.1 × 10-7 M and 5.1 × 10-6 M, respectively). This is in contrast to the signal transduction pathways described for the related tyramine- and alpha 2-adrenergic receptors, which couple in an inhibitory way to adenylyl cyclase.

Copyright © by The American Society for Pharmacology and Experimental Therapeutics


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