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Vol. 53, Issue 2, 274-282, February 1998
Department of Pharmacology and Toxicology, University of Alabama at
Birmingham, Birmingham, Alabama 35294
Sulfation is an important conjugation reaction for a wide range of
endogenous and exogenous compounds in humans, including steroids, bile
acids, catecholamine neurotransmitters and thyroid hormones. The cDNA
for a distinct human cytosolic sulfotransferase (ST), hST1B2, has been
isolated from a human liver 
Zap cDNA library. The hST1B2 cDNA
consists of 1144 bp and contains the coding region for a novel human
cytosolic ST that has been termed hST1B2 on the basis of its sequence
similarity to a rat sulfotransferase, ST1B1. The hST1B2 cDNA contains
an 888-bp open reading frame that encodes a 296-amino acid protein with
a calculated molecular mass of 34,897 Da. The hST1B2 cDNA also has a
127-bp 5
untranslated region (UTR) and a 129-bp 3-UTR, including a
22-bp poly(A)+ tract. The amino acid sequence of hST1B2 is
74%, 53%, 53%, 52%, 56%, and 34% identical to the amino acid
sequences of rat ST1B1 and human P-PST-1, P-PST-2, M-PST, EST, and
DHEA-ST, respectively. Enzymatically active hST1B2 was expressed in the
bacterial expression vector pKK233-2 for kinetic characterization and
in the bacterial expression vector pQE-31, which generates a
histidine-tagged fusion protein for the generation of antibodies.
Expressed hST1B2 sulfates small phenols such as 1-naphthol and
p-nitrophenol and thyroid hormones, including
3,3-diiodothyronine, triiodothyronine, reverse triiodothyronine, and
thyroxine. No activity was detected when several steroids or dopamine
were tested as substrates. High levels of hST1B2 message were detected
by Northern blot analysis in RNA isolated from human liver, colon,
small intestine, and blood leukocytes. Immunoblot analysis detected a
protein with the same mass as expressed hST1B2 in several human tissues
that also possessed hST1B2 message. These results indicate that a novel
cytosolic ST is present in human tissues, which may have an important
role in thyroid hormone and xenobiotic metabolism.
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