![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 53, Issue 6, 1104-1111, June 1998
Laboratoire de Biophysique Moléculaire et Cellulaire (C.A.), CNRS
URA 520, CEA/Grenoble, 38054 Grenoble, France,
Laboratoire Canaux
Ioniques et Signalisation (M.V.), CEA-DBMS/Grenoble, 38054 Grenoble,
France, and
Department of Anatomy and Cellular Biology (H.M.F.), Tufts
University School of Medicine, Boston, Massachusetts 02111
The effects of pharmacological agents on the T-type Ca2+
current were studied in dissociated spermatogenic cells from the mouse. Ca2+ currents were elicited by depolarization in 10 mM Ca2+ and recorded in the whole-cell
configuration of the patch clamp technique. The T-type current was
inhibited by the following compounds: PN200-110 (IC50 = 4 × 10
8 M) > nifedipine (IC50 = 4 × 107 M) > pimozide (IC50 = 4.6 × 107 M) > mibefradil (IC50 = 5 × 106 M) > Ni2+ (IC50 = 3.4 × 105 M) > verapamil (IC50 = 7 × 105 M) > amiloride (IC50 = 2.4 × 104 M) > Cd2+ (IC50 = 2.8 × 104 M). However, the agents differed in the
reversibility and the use dependence of their effects. Currents
recovered rapidly and completely after removal of
Ni2+, Cd2+, amiloride, or mibefradil,
whereas recovery from verapamil block was rapid but incomplete. In
contrast, we observed little recovery after the removal of pimozide and
of the dihydropyridines (PN200-110, nifedipine). Moreover, mibefradil
and pimozide exhibit a strongly use-dependent inhibition of current
that is due to selective interaction of these drugs with the open state
and the inactivated state of the channel, respectively, rather than
with the resting state. These properties of the spermatogenic T-type
Ca2+ channel differ from those of somatic cell T channels
and suggest a molecular diversity of low voltage-activated
Ca2+ channels.
This article has been cited by other articles:
|
|
 |
|
  S.-K. Yang, H. C. Parkington, J. Epelbaum, D. J. Keating, and C. Chen Somatostatin decreases voltage-gated Ca2+ currents in GH3 cells through activation of somatostatin receptor 2 Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1863 - E1870. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. S. Freeze, M. M. McNulty, and D. A. Hanck State-Dependent Verapamil Block of the Cloned Human Cav3.1 T-Type Ca2+ Channel Mol. Pharmacol., August 1, 2006; 70(2): 718 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Jimenez-Gonzalez, F. Michelangeli, C.V. Harper, C.L.R. Barratt, and S.J. Publicover Calcium signalling in human spermatozoa: a specialized 'toolkit' of channels, transporters and stores Hum. Reprod. Update, May 1, 2006; 12(3): 253 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-G. Feng and L. G. Navar Nitric oxide synthase inhibition activates L- and T-type Ca2+ channels in afferent and efferent arterioles Am J Physiol Renal Physiol, April 1, 2006; 290(4): F873 - F879. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. O. Hernandez-Gonzalez, J. Sosnik, J. Edwards, J. J. Acevedo, I. Mendoza-Lujambio, I. Lopez-Gonzalez, I. Demarco, E. Wertheimer, A. Darszon, and P. E. Visconti Sodium and Epithelial Sodium Channels Participate in the Regulation of the Capacitation-associated Hyperpolarization in Mouse Sperm J. Biol. Chem., March 3, 2006; 281(9): 5623 - 5633. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Price, E. L. Hansen, and T. N. Oliver Immunofluorescent Localization of a Novel Progesterone Receptor(s) in a T47D-Y Breast Cancer Cell Line Lacking Genomic Progesterone Receptor Expression Reproductive Sciences, December 1, 2005; 12(8): 610 - 616. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Felix Molecular physiology and pathology of Ca2+-conducting channels in the plasma membrane of mammalian sperm Reproduction, March 1, 2005; 129(3): 251 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. F. Hanna, C. L. Kerr, J. H. Shaper, and W. W. Wright Lewis X-Containing Neoglycoproteins Mimic the Intrinsic Ability of Zona Pellucida Glycoprotein ZP3 to Induce the Acrosome Reaction in Capacitated Mouse Sperm Biol Reprod, September 1, 2004; 71(3): 778 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Leresche, J. Hering, and R. C. Lambert Paradoxical Potentiation of Neuronal T-Type Ca2+ Current by ATP at Resting Membrane Potential J. Neurosci., June 16, 2004; 24(24): 5592 - 5602. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Brault, Z. Amiar, A.-M. Pennarun, M. Monestiez, Z. Zhang, D. Cornel, O. Dellis, H. Knight, F. Bouteau, and J.-P. Rona Plasma Membrane Depolarization Induced by Abscisic Acid in Arabidopsis Suspension Cells Involves Reduction of Proton Pumping in Addition to Anion Channel Activation, Which Are Both Ca2+ Dependent Plant Physiology, May 1, 2004; 135(1): 231 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-G. Feng, M. Li, and L. G. Navar T-type calcium channels in the regulation of afferent and efferent arterioles in rats Am J Physiol Renal Physiol, February 1, 2004; 286(2): F331 - F337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Turner Tales From the Tail: What Do We Really Know About Sperm Motility? J Androl, November 1, 2003; 24(6): 790 - 803. [Full Text] [PDF]
|
|
|
|
 |
|
 E. Perez-Reyes Molecular Physiology of Low-Voltage-Activated T-type Calcium Channels Physiol Rev, January 1, 2003; 83(1): 117 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. E. Bertolesi, C. Shi, L. Elbaum, C. Jollimore, G. Rozenberg, S. Barnes, and M. E. M. Kelly The Ca2+ Channel Antagonists Mibefradil and Pimozide Inhibit Cell Growth via Different Cytotoxic Mechanisms Mol. Pharmacol., August 1, 2002; 62(2): 210 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hirooka, G. E. Bertolesi, M. E. M. Kelly, E. M. Denovan-Wright, X. Sun, J. Hamid, G. W. Zamponi, A. E. Juhasz, L. W. Haynes, and S. Barnes T-Type Calcium Channel alpha 1G and alpha 1H Subunits in Human Retinoblastoma Cells and Their Loss After Differentiation J Neurophysiol, July 1, 2002; 88(1): 196 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Kirkman-Brown, E. L. Punt, C. L. R. Barratt, and S. J. Publicover Zona Pellucida and Progesterone-Induced Ca2+ Signaling and Acrosome Reaction in Human Spermatozoa J Androl, May 1, 2002; 23(3): 306 - 315. [Full Text] [PDF]
|
|
|
|
|
|
S. Jagannathan, E. L. Punt, Y. Gu, C. Arnoult, D. Sakkas, C. L. R. Barratt, and S. J. Publicover Identification and Localization of T-type Voltage-operated Calcium Channel Subunits in Human Male Germ Cells. EXPRESSION OF MULTIPLE ISOFORMS J. Biol. Chem., March 1, 2002; 277(10): 8449 - 8456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Santi, F. S. Cayabyab, K. G. Sutton, J. E. McRory, J. Mezeyova, K. S. Hamming, D. Parker, A. Stea, and T. P. Snutch Differential Inhibition of T-Type Calcium Channels by Neuroleptics J. Neurosci., January 15, 2002; 22(2): 396 - 403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Andreasen, B. L. Jensen, P. B. Hansen, T.-H. Kwon, S. Nielsen, and O. Skott The alpha 1G-subunit of a voltage-dependent Ca2+ channel is localized in rat distal nephron and collecting duct Am J Physiol Renal Physiol, December 1, 2000; 279(6): F997 - F1005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Martin, J.-H. Lee, L. L. Cribbs, E. Perez-Reyes, and D. A. Hanck Mibefradil Block of Cloned T-Type Calcium Channels J. Pharmacol. Exp. Ther., October 1, 2000; 295(1): 302 - 308. [Abstract] [Full Text]
|
|
|
|
 |
|
 W.-Y. Son, J.-H. Lee, J.-H. Lee, and C.-T. Han Acrosome reaction of human spermatozoa is mainly mediated by {alpha}1H T-type calcium channels Mol. Hum. Reprod., October 1, 2000; 6(10): 893 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Morales, E. Pizarro, M. Kong, B. Kerr, F. Ceric, and P. Vigil Gonadotropin-Releasing Hormone-Stimulated Sperm Binding to the Human Zona Is Mediated by a Calcium Influx Biol Reprod, August 1, 2000; 63(2): 635 - 642. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. M.B. O'Toole, C. Arnoult, A. Darszon, R. A. Steinhardt, and H. M. Florman Ca2+ Entry through Store-operated Channels in Mouse Sperm Is Initiated by Egg ZP3 and Drives the Acrosome Reaction Mol. Biol. Cell, May 1, 2000; 11(5): 1571 - 1584. [Abstract] [Full Text]
|
|
|
|
|
|
S. Wu, M. Zhang, P. A. Vest, A. Bhattacharjee, L. Liu, and M. Li A Mibefradil Metabolite Is a Potent Intracellular Blocker of L-Type Ca2+ Currents in Pancreatic beta -Cells J. Pharmacol. Exp. Ther., March 1, 2000; 292(3): 939 - 943. [Abstract] [Full Text]
|
|
|
|
|
|
J. C. Gomora, L. Xu, J. A. Enyeart, and J. J. Enyeart Effect of Mibefradil on Voltage-Dependent Gating and Kinetics of T-Type Ca2+ Channels in Cortisol-Secreting Cells J. Pharmacol. Exp. Ther., January 1, 2000; 292(1): 96 - 103. [Abstract] [Full Text]
|
|
|
|
|
|
J. C. Gomora, J. A. Enyeart, and J. J. Enyeart Mibefradil Potently Blocks ATP-Activated K+ Channels in Adrenal Cells Mol. Pharmacol., December 1, 1999; 56(6): 1192 - 1197. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Arnoult, I. G. Kazam, P. E. Visconti, G. S. Kopf, M. Villaz, and H. M. Florman Control of the low voltage-activated calcium channel of mouse sperm by egg ZP3 and by membrane hyperpolarization during capacitation PNAS, June 8, 1999; 96(12): 6757 - 6762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.J. Publicover and C.L.R. Barratt Voltage-operated Ca2+ channels and the acrosome reaction: which channels are present and what do they do? Hum. Reprod., April 1, 1999; 14(4): 873 - 879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Darszon, P. Labarca, T. Nishigaki, and F. Espinosa Ion Channels in Sperm Physiology Physiol Rev, April 1, 1999; 79(2): 481 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Garcia and S. Meizel Progesterone-Mediated Calcium Influx and Acrosome Reaction of Human Spermatozoa: Pharmacological Investigation of T-Type Calcium Channels Biol Reprod, January 1, 1999; 60(1): 102 - 109. [Abstract] [Full Text]
|
|
|
|
|
|
G. Wennemuth, R. E. Westenbroek, T. Xu, B. Hille, and D. F. Babcock CaV2.2 and CaV2.3 (N- and R-type) Ca2+ Channels in Depolarization-evoked Entry of Ca2+ into Mouse Sperm J. Biol. Chem., July 7, 2000; 275(28): 21210 - 21217. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
