MolPharm xPharm- The Comprehensive Pharmacology Reference

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boss, V.
Right arrow Articles by Murphy, T. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boss, V.
Right arrow Articles by Murphy, T. J.

Vol. 54, Issue 2, 264-272, August 1998

Histamine Induces Nuclear Factor of Activated T Cell-Mediated Transcription and Cyclosporin A-Sensitive Interleukin-8 mRNA Expression in Human Umbilical Vein Endothelial Cells

Valerie Boss, Xiaofei Wang, Lacie F. Koppelman, Kaiming Xu, and T. J. Murphy

Department of Pharmacology and Program in Molecular Therapeutics and Toxicology, Graduate Division of Biological and Biomedical Sciences (X.W., T.J.M.), Emory University School of Medicine, Atlanta, Georgia 30322

The nuclear factor of activated T cells (NFAT) mediates a cyclosporin A (CsA)- and FK506-suppressible transcriptional program in lymphocytes after antigen-stimulated phospholipase C activation. Nonlymphoid cells also express NFAT isoforms, raising the possibility that these isoforms can be regulated by other extracellular stimuli. This study sought to determine whether histamine can trigger NFAT-mediated transcription in human umbilical vein endothelial cells (HUVEC), using a retrovirus-based luciferase reporter driven by a well characterized, NFAT-specific enhancer. Luciferase levels are induced up to 60-fold over basal levels after costimulation of HUVEC with Ca2+-mobilizing drugs and a phorbol ester, a response that is 20-fold greater than that observed when HUVEC are stimulated with either drug alone. These synergistic responses are inhibited in cells treated with CsA. CsA and FK506 also inhibit the luciferase response to histamine, indicating that histamine can induce NFAT-mediated transcription in HUVEC. To identify candidate genes in HUVEC that might be regulated by NFAT, the expression of several chemokine mRNAs was measured after histamine treatment. Of the mRNAs tested, only those encoding monocyte chemotactic protein-1 (~2-fold over basal) and interleukin-8 (~6-fold over basal) are induced by histamine; both of these responses are suppressed by CsA and FK506. The H1 histamine receptor antagonist chlorpheniramine, but not the H2 receptor antagonist ranitidine, blocks the effects of histamine in this preparation. These data provide the first evidence for a physiological inducer of NFAT-mediated transcription in endothelial cells and support the hypothesis that NFAT participates in H1 histamine receptor-induced interleukin-8 gene expression.

Copyright © 1998 by The American Society for Pharmacology and Experimental Therapeutics


This article has been cited by other articles:


Home page
 J. Virol.Home page
M. M. Brinkmann, M. Pietrek, O. Dittrich-Breiholz, M. Kracht, and T. F. Schulz
Modulation of Host Gene Expression by the K15 Protein of Kaposi's Sarcoma-Associated Herpesvirus
J. Virol., January 1, 2007; 81(1): 42 - 58.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Uzonyi, K. Lotzer, S. Jahn, C. Kramer, M. Hildner, E. Bretschneider, D. Radke, M. Beer, R. Vollandt, J. F. Evans, et al.
Cysteinyl leukotriene 2 receptor and protease-activated receptor 1 activate strongly correlated early genes in human endothelial cells
PNAS, April 18, 2006; 103(16): 6326 - 6331.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. H. Cleator, W. Q. Zhu, D. E. Vaughan, and H. E. Hamm
Differential regulation of endothelial exocytosis of P-selectin and von Willebrand factor by protease-activated receptors and cAMP
Blood, April 1, 2006; 107(7): 2736 - 2744.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Minami, K. Horiuchi, M. Miura, Md. R. Abid, W. Takabe, N. Noguchi, T. Kohro, X. Ge, H. Aburatani, T. Hamakubo, et al.
Vascular Endothelial Growth Factor- and Thrombin-induced Termination Factor, Down Syndrome Critical Region-1, Attenuates Endothelial Cell Proliferation and Angiogenesis
J. Biol. Chem., November 26, 2004; 279(48): 50537 - 50554.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
T. M. Thway, S. G. Shlykov, M.-C. Day, B. M. Sanborn, L. C. Gilstrap III, Y. Xia, and R. E. Kellems
Antibodies From Preeclamptic Patients Stimulate Increased Intracellular Ca2+ Mobilization Through Angiotensin Receptor Activation
Circulation, September 21, 2004; 110(12): 1612 - 1619.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
B. A. Hesser, X. H. Liang, G. Camenisch, S. Yang, D. A. Lewin, R. Scheller, N. Ferrara, and H.-P. Gerber
Down syndrome critical region protein 1 (DSCR1), a novel VEGF target gene that regulates expression of inflammatory markers on activated endothelial cells
Blood, July 1, 2004; 104(1): 149 - 158.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
T. Wierzbicki, S. M. Iqbal, S. L. Cuvelier, G. Awong, L. A. Tibbles, and K. D. Patel
IL-4 primes human endothelial cells for secondary responses to histamine
J. Leukoc. Biol., September 1, 2003; 74(3): 420 - 427.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. F. Gomez, A. S. Stevenson, A. D. Bonev, D. C. Hill-Eubanks, and M. T. Nelson
Opposing Actions of Inositol 1,4,5-Trisphosphate and Ryanodine Receptors on Nuclear Factor of Activated T-cells Regulation in Smooth Muscle
J. Biol. Chem., September 27, 2002; 277(40): 37756 - 37764.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
V. N. Bochkov, D. Mechtcheriakova, M. Lucerna, J. Huber, R. Malli, W. F. Graier, E. Hofer, B. R. Binder, and N. Leitinger
Oxidized phospholipids stimulate tissue factor expression in human endothelial cells via activation of ERK/EGR-1 and Ca++/NFAT
Blood, January 1, 2002; 99(1): 199 - 206.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
K. L. Abbott, J. R. Loss II, A. M. Robida, and T. J. Murphy
Evidence That Galpha q-Coupled Receptor-Induced Interleukin-6 mRNA in Vascular Smooth Muscle Cells Involves the Nuclear Factor of Activated T Cells
Mol. Pharmacol., November 1, 2000; 58(5): 946 - 953.
[Abstract] [Full Text]

Home page
 Mol. Pharmacol.Home page
A. M. Robida, K. Xu, M. L. Ellington, and T. J. Murphy
Cyclosporin A Selectively Inhibits Mitogen-Induced Cyclooxygenase-2 Gene Transcription in Vascular Smooth Muscle Cells
Mol. Pharmacol., October 1, 2000; 58(4): 701 - 708.
[Abstract] [Full Text]

Home page
 J. Exp. Med.Home page
R. K. Srivastava, C. Y. Sasaki, J. M. Hardwick, and D. L. Longo
Bcl-2–mediated Drug Resistance: Inhibition of Apoptosis by Blocking Nuclear Factor of Activated T Lymphocytes (NFAT)-induced Fas Ligand Transcription
J. Exp. Med., July 19, 1999; 190(2): 253 - 266.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1998 by the American Society for Pharmacology and Experimental Therapeutics