MolPharm xPharm- The Comprehensive Pharmacology Reference

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Woods, M.
Right arrow Articles by Warner, T. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Woods, M.
Right arrow Articles by Warner, T. D.

Vol. 55, Issue 5, 902-909, May 1999

Endothelin-1 Is Induced by Cytokines in Human Vascular Smooth Muscle Cells: Evidence for Intracellular Endothelin-Converting Enzyme

Mandy Woods, Jane A. Mitchell, Elizabeth G. Wood, Stewart Barker, Nicholas R. Walcot, Gareth M. Rees, and Timothy D. Warner

Vascular Inflammation, The William Harvey Research Institute, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Charterhouse Square, London (M.W., E.G.W., S.B., N.R.W., T.D.W.); Department of Critical Care Medicine, National Heart and Lung Institute, Imperial College, Sydney Street, London (J.A.M.); and Department of Cardiothoracic Surgery, St. Bartholomew's Hospital, West Smithfield, London (G.M.R.), United Kingdom

Endothelin-1 (ET-1) is the predominant endothelin isopeptide generated by the vascular wall and therefore appears to be the most important peptide involved in regulation of cardiovascular events. Many pathologic conditions are associated with elevations of ET-1 in the blood vessel wall. Because these conditions are often cytokine driven, we examined the effects of a mixture of cytokines on ET-1 production in human vascular smooth muscle cells (VSMCs) derived from internal mammary artery and saphenous vein (SV). Incubation of IMA and SV VSMCs with tumor necrosis factor-alpha (10 ng/ml) and interferon-gamma (1000 U/ml) in combination for up to 48 h markedly elevated the expression of mRNA for prepro-ET-1 and the release of ET-1 into the culture medium. This cytokine-stimulated release of ET-1 was inhibited by a series of dual endothelin-converting enzyme (ECE)/neutral endopeptidase inhibitors, phosphoramidon, CGS 26303, and CGS 26393, with an accompanying increase in big ET-1 release but with no effect on expression of mRNA for prepro-ET-1. These same compounds were 10 times more potent at inhibiting the conversion of exogenously applied big ET-1 to ET-1. ECE-1b/c mRNA is present in SV VSMCs, however no ECE-1a is present in these cells. Thus VSMCs most probably contain, like endothelial cells, an intracellular ECE responsible for the endogenous synthesis of ET-1. Under the influence of pro-inflammatory mediators the vascular smooth muscle can therefore become an important site of ET-1 production, as has already been established for the dilator mediators nitric oxide, prostaglandin I2, and prostaglandin E2.

Copyright © 1999 by The American Society for Pharmacology and Experimental Therapeutics


This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
W. Wang, H. Yen, C.-H. Chen, R. Soni, N. Jasani, G. Sylvestre, and S. E. Reznik
The Endothelin-Converting Enzyme-1/Endothelin-1 Pathway Plays a Critical Role in Inflammation-Associated Premature Delivery in a Mouse Model
Am. J. Pathol., October 1, 2008; 173(4): 1077 - 1084.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes Spectr.Home page
B. B. Dokken
The Pathophysiology of Cardiovascular Disease and Diabetes: Beyond Blood Pressure and Lipids
Diabetes Spectr, July 1, 2008; 21(3): 160 - 165.
[Abstract] [Full Text] [PDF]

Home page
 Br J AnaesthHome page
J. X. Wei, A. Verity, M. Garle, R. Mahajan, and V. Wilson
Examination of the effect of procalcitonin on human leucocytes and the porcine isolated coronary artery
Br. J. Anaesth., May 1, 2008; 100(5): 612 - 621.
[Abstract] [Full Text] [PDF]

Home page
 The Annals of PharmacotherapyHome page
R. P Miech
Pathopharmacology of Excessive Hemorrhage in Mifepristone Abortions
Ann. Pharmacother., December 1, 2007; 41(12): 2002 - 2007.
[Abstract] [Full Text] [PDF]

Home page
 Br J AnaesthHome page
W. Qi, J. X. Wei, I. Dorairaj, R. P. Mahajan, and V. G. Wilson
Evidence that a prostanoid produced by cyclo-oxygenase-2 enhances contractile responses of the porcine isolated coronary artery following exposure to lipopolysaccharide
Br. J. Anaesth., March 1, 2007; 98(3): 323 - 330.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
T. Lattmann, J. Ortmann, S. Horber, S. G. Shaw, M. Hein, and M. Barton
Upregulation of endothelin converting enzyme-1 in host liver during chronic cardiac allograft rejection.
Experimental Biology and Medicine, June 1, 2006; 231(6): 899 - 901.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J. J. Lepore, T. P. Cappola, P. A. Mericko, E. E. Morrisey, and M. S. Parmacek
GATA-6 Regulates Genes Promoting Synthetic Functions in Vascular Smooth Muscle Cells
Arterioscler. Thromb. Vasc. Biol., February 1, 2005; 25(2): 309 - 314.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. A. Gupte, E. A. Zias, M. R. Sarabu, and M. S. Wolin
Role of Prostaglandins in Mediating Differences in Human Internal Mammary and Radial Artery Relaxation Elicited by Hypoxia
J. Pharmacol. Exp. Ther., November 1, 2004; 311(2): 510 - 518.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. D. Moraes, G. Gioseffi, A. C. L. Nobrega, and E. Tibirica
Effects of exercise training on the vascular reactivity of the whole kidney circulation in rabbits
J Appl Physiol, August 1, 2004; 97(2): 683 - 688.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
A. Carotti, F. Emma, S. Picca, E. Iannace, S. B. Albanese, M. Grigioni, F. Meo, M. Sciarra, and R. M. Di Donato
Inflammatory response to cardiac bypass in ewe fetuses: effects of steroid administration or continuous hemodiafiltration
J. Thorac. Cardiovasc. Surg., December 1, 2003; 126(6): 1839 - 1848.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Narayan, G. Prasanna, R. R. Krishnamoorthy, X. Zhang, and T. Yorio
Endothelin-1 Synthesis and Secretion in Human Retinal Pigment Epithelial Cells (ARPE-19): Differential Regulation by Cholinergics and TNF-{alpha}
Invest. Ophthalmol. Vis. Sci., November 1, 2003; 44(11): 4885 - 4894.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
M. Woods, E. G. Wood, S. C. Bardswell, D. Bishop-Bailey, S. Barker, S. J. Wort, J. A. Mitchell, and T. D. Warner
Role for Nuclear Factor-{kappa}B and Signal Transducer and Activator of Transcription 1/Interferon Regulatory Factor-1 in Cytokine-Induced Endothelin-1 Release in Human Vascular Smooth Muscle Cells
Mol. Pharmacol., October 1, 2003; 64(4): 923 - 931.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
E.D. Moloney and T.W. Evans
Pathophysiology and pharmacological treatment of pulmonary hypertension in acute respiratory distress syndrome
Eur. Respir. J., April 1, 2003; 21(4): 720 - 727.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
N. Levy, M. Gordin, M. F. Smith, O. U. Bolden-Tiller, and R. Meidan
Hormonal Regulation and Cell-Specific Expression of Endothelin-Converting Enzyme 1 Isoforms in Bovine Ovarian Endothelial and Steroidogenic Cells
Biol Reprod, April 1, 2003; 68(4): 1361 - 1368.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
S. J. Wort, M. Woods, T. D. Warner, T. W. Evans, and J. A. Mitchell
Cyclooxygenase-2 Acts as an Endogenous Brake on Endothelin-1 Release by Human Pulmonary Artery Smooth Muscle Cells: Implications for Pulmonary Hypertension
Mol. Pharmacol., November 1, 2002; 62(5): 1147 - 1153.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
N. DAVIE, S. J. HALEEN, P. D. UPTON, J. M. POLAK, M. H. YACOUB, N. W. MORRELL, and J. WHARTON
ETA and ETB Receptors Modulate the Proliferation of Human Pulmonary Artery Smooth Muscle Cells
Am. J. Respir. Crit. Care Med., February 1, 2002; 165(3): 398 - 405.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
H.-D. Orzechowski, A. Gunther, S. Menzel, A. Zimmermann, H. Funke-Kaiser, R. Real, T. Subkowski, F. S. Zollmann, and M. Paul
Transcriptional Mechanism of Protein Kinase C-Induced Isoform-Specific Expression of the Gene for Endothelin-Converting Enzyme-1in Human Endothelial Cells
Mol. Pharmacol., December 1, 2001; 60(6): 1332 - 1342.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
S. Kinlay, D. Behrendt, M. Wainstein, J. Beltrame, J. C. Fang, M. A. Creager, A. P. Selwyn, and P. Ganz
Role of Endothelin-1 in the Active Constriction of Human Atherosclerotic Coronary Arteries
Circulation, September 4, 2001; 104(10): 1114 - 1118.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. J. Wort, M. Woods, T. D. Warner, T. W. Evans, and J. A. Mitchell
Endogenously Released Endothelin-1 from Human Pulmonary Artery Smooth Muscle Promotes Cellular Proliferation . Relevance to Pathogenesis of Pulmonary Hypertension and Vascular Remodeling
Am. J. Respir. Cell Mol. Biol., July 1, 2001; 25(1): 104 - 110.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
S. Barker, N. Q. Khan, E. G. Wood, and R. Corder
Effect of an Antisense Oligodeoxynucleotide to Endothelin-Converting Enzyme-1c (ECE-1c) on ECE-1c mRNA, ECE-1 Protein and Endothelin-1 Synthesis in Bovine Pulmonary Artery Smooth Muscle Cells
Mol. Pharmacol., February 1, 2001; 59(2): 163 - 169.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
A. Friedman, S. Weiss, N. Levy, and R. Meidan
Role of Tumor Necrosis Factor {alpha} and Its Type I Receptor in Luteal Regression: Induction of Programmed Cell Death in Bovine Corpus Luteum-Derived Endothelial Cells
Biol Reprod, December 1, 2000; 63(6): 1905 - 1912.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. Ergul, C. A. Walker, A. Goldberg, S. C. Baicu, J. W. Hendrick, M. K. King, and F. G. Spinale
ET-1 in the myocardial interstitium: relation to myocyte ECE activity and expression
Am J Physiol Heart Circ Physiol, June 1, 2000; 278(6): H2050 - H2056.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
C. Ferri, G. Properzi, G. Tomassoni, A. Santucci, G. Desideri, A. E. Giuliani, R. C. Starling, N. B. Ratliff, D. J. Cook, P. McCarthy, et al.
Patterns of Myocardial Endothelin-1 Expression and Outcome After Cardiac Transplantation
Circulation, April 16, 2002; 105(15): 1768 - 1771.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1999 by the American Society for Pharmacology and Experimental Therapeutics