Abstract
Different amino side chains in the N-terminal extracellular region of the inhibitory glycine receptor (GlyR) have been shown to be crucial for ligand recognition. Here we describe a novel domain of the GlyRα1 subunit that constitutes an important determinant of antagonist activity. The antagonists strychnine, nipecotic acid, and isobutyric acid displayed reduced potencies at recombinant GlyRs formed from α1 subunits, in which lysine 104, phenylalanine 108, or threonine 112 were replaced by alanine. Agonist affinities, in contrast, were slightly increased at these mutant receptors. Taurine and β-aminoisobutyric acid, which are partial agonists at the wild-type GlyR, behaved as full agonists at the mutant GlyRs and failed to inhibit glycine-induced currents. This is consistent with apolar residues at positions 104, 108, and 112 of the α1 subunit reducing the antagonistic, but not the agonistic, binding of β-amino acids. Our data support a model in which the partial agonism of β-amino acids results from their self-inhibitory activity.
Footnotes
- Received March 8, 1999.
- Accepted June 16, 1999.
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Send reprint requests to: Dr. Volker Schmieden, Department of Physiology, Campus Charité, Humboldt-University of Berlin, Tucholskystrasse 2, 10117 Berlin, Germany. E-mail:volker.schmieden{at}charite.de
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↵1 Present address: Department of Physiology, Campus Charité, Humboldt-University of Berlin, Tucholskystrasse 2, 10117 Berlin, Federal Republic of Germany. Phone: +49(30)28026183, Fax +49(30)28026669, e-mail: volker.schmieden{at}charite.de
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↵2 Present address: Department of Anatomy and Cellular Neurobiology, University of Ulm, Albert-Einstein-Allee 11, D-89081 Ulm, Federal Republic of Germany.
- The American Society for Pharmacology and Experimental Therapeutics
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