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Vol. 57, Issue 2, 401-408, February 2000
-Opioid Receptor
Expression
Department of Pharmacology, University of Minnesota Medical School,
Minneapolis, Minnesota.
Three mRNA variants are generated from the mouse 
-opioid receptor
(KOR) gene. The expression patterns of these KOR mRNA variants in adult
animal tissues and during developmental stages are examined. Furthermore, the biological significance of generating these variants is demonstrated with respect to two post-transcriptional mechanisms, i.e., mRNA stability and translation efficiency. Variants A and B are
both transcribed from promoter 1 of the KOR gene and expressed from
early developmental stages through adult life. Although their sequences
differ only at a 30-nucleotide insertion for variant B, these two
variants are distinct with regard to their expression patterns, mRNA
stability, and translation efficiency. Variant A is expressed
ubiquitously in all the tissues examined and has a longer
t1/2 (12 h), whereas variant B is more
specific to the central nervous system both pre- and postnatally and
has a t1/2 of ~8 h. Variant C is
transcribed from promoter 2 of the KOR gene and is most specifically
expressed, being detected only in the brain stem, spinal cord, and
thalamic/hypothalamic areas of postnatal animals. With regard to
protein translation, variants B and C are significantly more efficient
than variant A. This study provides the evidence for multiple levels of
KOR regulation. The biological implication of the generation of KOR
mRNA variants is discussed.
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