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Vol. 57, Issue 5, 1064-1074, May 2000
1B Ca2+ Channels
Department of Neurobiology, Pharmacology, and Physiology, and
Committee on Neurobiology, The University of Chicago, Chicago, Illinois
The structural basis of Ca2+ channel inhibition by G
proteins has received considerable attention recently, and multiple
regions on Ca2+ channels that interact with G protein
subunits have been identified. We have demonstrated previously that a
region extending from the N terminus to the I/II loop of the
Ca2+ channel is involved in determining the differences
between 
1B and 1E Ca2+ channels with respect to
inhibition by G proteins. Here we explore this region of the
channel in greater detail in an effort to further define the regions
involved in determining inhibition. Chimeric Ca2+ channels
constructed from 1B and 1E Ca2+ channels revealed
that the N terminus, the I/II loop, and domain I all play an important
role in determining inhibition. We identified a 70-amino acid fragment
from domain I that mediates the effects of domain I, and a 50-amino
acid fragment from the I/II loop that mediates the effects of the I/II
loop. When these regions from 1B were exchanged into 1E,
inhibition identical with that of 1B was observed. The differences
between 1B and 1E in the identified region of domain I involve
residues that are predicted to be almost exclusively extracellular.
Mutations to some of the high-affinity G protein binding regions of
1B ( interaction domain, CC14, and a C-terminal G
binding site) caused relatively little change in inhibition, which
suggests that these sites are not necessary individually for G
protein-mediated inhibition and may help to explain the small effects
of exchanging these regions in isolation.
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