![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 58, Issue 5, 946-953, November 2000
q-Coupled Receptor-Induced
Interleukin-6 mRNA in Vascular Smooth Muscle Cells Involves the Nuclear
Factor of Activated T Cells
Department of Pharmacology and Graduate Program in Molecular and
Systems Pharmacology, Emory University School of Medicine,
Atlanta, Georgia
The immunosuppressant cyclosporin A inhibits transcription mediated by
the nuclear factor of activated T-cells (NFAT), a key regulator
of cytokine gene expression in lymphocytes that integrates phospholipase C signaling. NFAT is also expressed in vascular smooth
muscle cells, but the genes it regulates there are unknown. Here we
show that G
q-coupled P2Y nucleotide receptor
signaling in rat vascular smooth muscle cells increases NFAT-mediated
luciferase reporter expression. It also induces interleukin (IL)-6 gene
expression but not other cytokine mRNAs including IL-1, IL-2, IL-3,
IL-4, IL-10, 
-interferon, tumor necrosis factor-, or tumor
necrosis factor-
. IL-6 mRNA induction by UTP is more rapid and
transient then that caused by IL-1 stimulation and is partially
blocked by cyclosporin A or by expression of a
trans-dominant NFAT inhibitor. Expression of recombinant
NFATc1 markedly augments IL-6 mRNA induction by these and other
agonists, which is partially attributable to NFAT-regulated paracrine
mediators. However, trans-dominant NF
B inhibitors
strongly interfere with IL-6 mRNA induction both by IL-1 and by UTP,
which synergistically evoke IL-6 mRNA expression. These findings
suggest that NFAT is among the cofactors involved in NFB-dependent
IL-6 gene induction by Ca2+-mobilizing receptors in
vascular smooth muscle cells.
This article has been cited by other articles:
|
|
 |
|
  S. Taurin, N. Sandbo, D. M. Yau, N. Sethakorn, and N. O. Dulin Phosphorylation of {beta}-catenin by PKA promotes ATP-induced proliferation of vascular smooth muscle cells Am J Physiol Cell Physiol, May 1, 2008; 294(5): C1169 - C1174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. de Frutos, L. Duling, D. Alo, T. Berry, O. Jackson-Weaver, M. Walker, N. Kanagy, and L. Gonzalez Bosc NFATc3 is required for intermittent hypoxia-induced hypertension Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2382 - H2390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Xu and H.-K. G. Shu EGFR Activation Results in Enhanced Cyclooxygenase-2 Expression through p38 Mitogen-Activated Protein Kinase-Dependent Activation of the Sp1/Sp3 Transcription Factors in Human Gliomas Cancer Res., July 1, 2007; 67(13): 6121 - 6129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. de Frutos, R. Spangler, D. Alo, and L. V. G. Bosc NFATc3 Mediates Chronic Hypoxia-induced Pulmonary Arterial Remodeling with {alpha}-Actin Up-regulation J. Biol. Chem., May 18, 2007; 282(20): 15081 - 15089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Nilsson, Z.-W. Sun, J. Nilsson, I. Nordstrom, Y.-W. Chen, J. D. Molkentin, D. Wide-Swensson, P. Hellstrand, M.-L. Lydrup, and M. F. Gomez Novel blocker of NFAT activation inhibits IL-6 production in human myometrial arteries and reduces vascular smooth muscle cell proliferation Am J Physiol Cell Physiol, March 1, 2007; 292(3): C1167 - C1178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. O'Connor, S. T. Mills, K. A. Jones, S. N. Ho, and G. K. Pavlath A combinatorial role for NFAT5 in both myoblast migration and differentiation during skeletal muscle myogenesis J. Cell Sci., January 1, 2007; 120(1): 149 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Douillet, W. P. Robinson III, P. M. Milano, R. C. Boucher, and P. B. Rich Nucleotides induce IL-6 release from human airway epithelia via P2Y2 and p38 MAPK-dependent pathways Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L734 - L746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-A. Renault, S. Jalvy, M. Potier, I. Belloc, E. Genot, L. V. Dekker, C. Desgranges, and A.-P. Gadeau UTP Induces Osteopontin Expression through a Coordinate Action of NF{kappa}B, Activator Protein-1, and Upstream Stimulatory Factor in Arterial Smooth Muscle Cells J. Biol. Chem., January 28, 2005; 280(4): 2708 - 2713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Liu, N. Dronadula, and G. N. Rao A Novel Role for Nuclear Factor of Activated T Cells in Receptor Tyrosine Kinase and G Protein-coupled Receptor Agonist-induced Vascular Smooth Muscle Cell Motility J. Biol. Chem., September 24, 2004; 279(39): 41218 - 41226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Schafer, F. Sedehizade, T. Welte, and G. Reiser ATP- and UTP-activated P2Y receptors differently regulate proliferation of human lung epithelial tumor cells Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L376 - L385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Gomez, A. S. Stevenson, A. D. Bonev, D. C. Hill-Eubanks, and M. T. Nelson Opposing Actions of Inositol 1,4,5-Trisphosphate and Ryanodine Receptors on Nuclear Factor of Activated T-cells Regulation in Smooth Muscle J. Biol. Chem., September 27, 2002; 277(40): 37756 - 37764. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
