![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 59, Issue 2, 278-284, February 2001
Pharmacogenetics Section, Laboratory of Reproductive and
Developmental Toxicology, National Institute of Environmental Health
Sciences, National Institutes of Health, Research Triangle Park, North
Carolina
The nuclear receptor constitutive active receptor (CAR) translocates
into liver nuclei after phenobarbital (PB) treatment, and activates the
conserved enhancer called the PB-response element module (PBREM) found
in CYP2B genes. We have examined whether CAR regulates
the dimorphic induction by PB of the CYP2B1 gene in
Wistar Kyoto (WKY) rats. Northern blot analysis showed that PB induced
CYP2B1 mRNA in male WKY rats but not female rats. An in situ injected
PBREM-luciferase reporter gene was activated by PB only in the male
livers. Western blot analysis revealed extremely low levels of CAR in
the cytosols of female livers compared with male counterparts. CAR was
accumulated in the liver nucleus of male rats in response to PB
treatment, whereas the receptor was barely detectable in the liver
nuclei of PB-induced females. These sexually dimorphic responses of
PBREM and CAR to PB treatment were not observed with Fisher 344 rats,
in which CYP2B1 mRNA was induced in both sexes. Thus, these results
indicate that CAR is a regulatory factor that leads to the sexual
dimorphic induction of CYP2B1 gene in WKY rats.
This article has been cited by other articles:
|
|
 |
|
  C. D. Fisher, L. M. Augustine, J. M. Maher, D. M. Nelson, A. L. Slitt, C. D. Klaassen, L. D. Lehman-McKeeman, and N. J. Cherrington Induction of Drug-Metabolizing Enzymes by Garlic and Allyl Sulfide Compounds via Activation of Constitutive Androstane Receptor and Nuclear Factor E2-Related Factor 2 Drug Metab. Dispos., June 1, 2007; 35(6): 995 - 1000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Anakk, W. Huang, J. L. Staudinger, K. Tan, T. J. Cole, D. D. Moore, and H. W. Strobel Gender Dictates the Nuclear Receptor-Mediated Regulation of CYP3A44 Drug Metab. Dispos., January 1, 2007; 35(1): 36 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Sakai, H. Iwata, E.-Y. Kim, O. Tsydenova, N. Miyazaki, E. A. Petrov, V. B. Batoev, and S. Tanabe Constitutive Androstane Receptor (CAR) as a Potential Sensing Biomarker of Persistent Organic Pollutants (POPs) in Aquatic Mammal: Molecular Characterization, Expression Level, and Ligand Profiling in Baikal Seal (Pusa sibirica) Toxicol. Sci., November 1, 2006; 94(1): 57 - 70. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Slitt, N. J. Cherrington, C. D. Fisher, M. Negishi, and C. D. Klaassen INDUCTION OF GENES FOR METABOLISM AND TRANSPORT BY TRANS-STILBENE OXIDE IN LIVERS OF SPRAGUE-DAWLEY AND WISTAR-KYOTO RATS Drug Metab. Dispos., July 1, 2006; 34(7): 1190 - 1197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Slitt, N. J. Cherrington, M. Z. Dieter, L. M. Aleksunes, G. L. Scheffer, W. Huang, D. D. Moore, and C. D. Klaassen trans-Stilbene Oxide Induces Expression of Genes Involved in Metabolism and Transport in Mouse Liver via CAR and Nrf2 Transcription Factors Mol. Pharmacol., May 1, 2006; 69(5): 1554 - 1563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Wyde, S. E. Kirwan, F. Zhang, A. Laughter, H. B. Hoffman, E. Bartolucci-Page, K. W. Gaido, B. Yan, and L. You Di-n-Butyl Phthalate Activates Constitutive Androstane Receptor and Pregnane X Receptor and Enhances the Expression of Steroid-Metabolizing Enzymes in the Liver of Rat Fetuses Toxicol. Sci., August 1, 2005; 86(2): 281 - 290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kobayashi, Y. Yamanaka, N. Iwazaki, I. Nakajo, M. Hosokawa, M. Negishi, and K. Chiba IDENTIFICATION OF HMG-CoA REDUCTASE INHIBITORS AS ACTIVATORS FOR HUMAN, MOUSE AND RAT CONSTITUTIVE ANDROSTANE RECEPTOR Drug Metab. Dispos., July 1, 2005; 33(7): 924 - 929. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. St-Pierre, T. Stallmach, A. Freimoser Grundschober, J.-F. Dufour, M. A. Serrano, J. J. G. Marin, Y. Sugiyama, and P. J. Meier Temporal expression profiles of organic anion transport proteins in placenta and fetal liver of the rat Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2004; 287(6): R1505 - R1516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wang, S. Faucette, R. Moore, T. Sueyoshi, M. Negishi, and E. LeCluyse Human Constitutive Androstane Receptor Mediates Induction of CYP2B6 Gene Expression by Phenytoin J. Biol. Chem., July 9, 2004; 279(28): 29295 - 29301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Handschin and U. A. Meyer Induction of Drug Metabolism: The Role of Nuclear Receptors Pharmacol. Rev., December 1, 2003; 55(4): 649 - 673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Cherrington, A. L. Slitt, J. M. Maher, X.-X. Zhang, J. Zhang, W. Huang, Y.-J. Y. Wan, D. D. Moore, and C. D. Klaassen INDUCTION OF MULTIDRUG RESISTANCE PROTEIN 3 (MRP3) IN VIVO IS INDEPENDENT OF CONSTITUTIVE ANDROSTANE RECEPTOR Drug Metab. Dispos., November 1, 2003; 31(11): 1315 - 1319. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Wyde, E. Bartolucci, A. Ueda, H. Zhang, B. Yan, M. Negishi, and L. You The Environmental Pollutant 1,1-Dichloro-2,2-bis (p-chlorophenyl)ethylene Induces Rat Hepatic Cytochrome P450 2B and 3A Expression through the Constitutive Androstane Receptor and Pregnane X Receptor Mol. Pharmacol., August 1, 2003; 64(2): 474 - 481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Degawa, M. Namiki, N. Yoshimoto, M. Makino, M. Iwamoto, K. Nemoto, and Y. Hashimoto Constitutive Expression of Cytochrome P450 Genes in Newly Established Rat Hepatic Cell Lines J. Biochem., June 1, 2003; 133(6): 825 - 831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Ledda-Columbano, M. Pibiri, D. Concas, F. Molotzu, G. Simbula, C. Cossu, and A. Columbano Sex difference in the proliferative response of mouse hepatocytes to treatment with the CAR ligand, TCPOBOP Carcinogenesis, June 1, 2003; 24(6): 1059 - 1065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Kocarek and N. A. Mercer-Haines Squalestatin 1-Inducible Expression of Rat CYP2B: Evidence That an Endogenous Isoprenoid Is an Activator of the Constitutive Androstane Receptor Mol. Pharmacol., November 1, 2002; 62(5): 1177 - 1186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Xiong, K. Yoshinari, K. L. R. Brouwer, and M. Negishi Role of Constitutive Androstane Receptor in the In Vivo Induction of Mrp3 and CYP2B1/2 by Phenobarbital Drug Metab. Dispos., August 1, 2002; 30(8): 918 - 923. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Van Ess, M. P. Mattson, and R. A. Blouin Enhanced Induction of Cytochrome P450 Enzymes and CAR Binding in TNF (p55-/-/p75-/-) Double Receptor Knockout Mice Following Phenobarbital Treatment J. Pharmacol. Exp. Ther., March 1, 2002; 300(3): 824 - 830. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
