![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 59, Issue 5, 1165-1170, May 2001
B in c-Myc Induction by Tubulin
Polymerization Inhibitors
Unité Mixte de Recherche Centre National de la
Recherche Scientifique 6032, Faculté de Pharmacie, Marseille,
France
We showed previously that microtubule disassembly by vinblastine
induces the proto-oncogene c-myc in epithelial mammary HBL100 cells
(Bourgarel-Rey et al., 2000). In this study, we demonstrate that
vinblastine treatment in these cells, in contrast to what was observed
with the colon adenocarcinoma cell line HT29-D4, activated the
transcription factor NF
B, which has been involved in c-myc
regulation. The microtubule disassembly also induced IB degradation.
Using transient transfection analysis, we show that the
trans-activation of c-myc by vinblastine was decreased when NFB binding sites on c-myc promoter were mutated. Additionally, we demonstrate that microtubule dissolution
trans-activated a thymidine kinase-CAT construct
containing an NFB binding site at 
1180 to 1080 bp relative to
the P1 promoter. Thus, vinblastine up-regulates the enhancer activity
of the NFB binding site. These results suggest that microtubule
disassembly induced by vinblastine can trans-activate
the c-myc oncogene through NFB. Taking into consideration the
paradoxical roles of both c-myc and NFB in proliferation or
apoptosis, this data reveals the complex action mechanism of this
microtubule interfering agent.
This article has been cited by other articles:
|
|
 |
|
  C. Wasylyk, H. Zheng, C. Castell, L. Debussche, M.-C. Multon, and B. Wasylyk Inhibition of the Ras-Net (Elk-3) Pathway by a Novel Pyrazole that Affects Microtubules Cancer Res., March 1, 2008; 68(5): 1275 - 1283. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Lee, S. M. Son, D. J. Son, S. M. Kim, T. J. Kim, S. Song, D. C. Moon, H. W. Lee, J. C. Ryu, D.-Y. Yoon, et al. Epothilones induce human colon cancer SW620 cell apoptosis via the tubulin polymerization independent activation of the nuclear factor-{kappa}B/I{kappa}B kinase signal pathway Mol. Cancer Ther., October 1, 2007; 6(10): 2786 - 2797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Izzo, U. Malhotra, T.-T. Wu, R. Luthra, A. M. Correa, S. G. Swisher, W. Hofstetter, K.S. C. Chao, M.-C. Hung, and J. A. Ajani Clinical Biology of Esophageal Adenocarcinoma after Surgery Is Influenced by Nuclear Factor-{kappa}B Expression Cancer Epidemiol. Biomarkers Prev., June 1, 2007; 16(6): 1200 - 1205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Fazal, M. Minhajuddin, K. M. Bijli, J. L. McGrath, and A. Rahman Evidence for Actin Cytoskeleton-dependent and -independent Pathways for RelA/p65 Nuclear Translocation in Endothelial Cells J. Biol. Chem., February 9, 2007; 282(6): 3940 - 3950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Arai, Y. Matsumoto, Y. Nagashima, and K. Yagasaki Regulation of Class II {beta}-Tubulin Expression by Tumor Suppressor p53 Protein in Mouse Melanoma Cells in Response to Vinca Alkaloid Mol. Cancer Res., April 1, 2006; 4(4): 247 - 255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Izzo, U. Malhotra, T.-T. Wu, J. Ensor, R. Luthra, J. H. Lee, S. G. Swisher, Z. Liao, K.S. C. Chao, W. N. Hittelman, et al. Association of Activated Transcription Factor Nuclear Factor {kappa}B With Chemoradiation Resistance and Poor Outcome in Esophageal Carcinoma J. Clin. Oncol., February 10, 2006; 24(5): 748 - 754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ghisletti, C. Meda, A. Maggi, and E. Vegeto 17{beta}-Estradiol Inhibits Inflammatory Gene Expression by Controlling NF-{kappa}B Intracellular Localization Mol. Cell. Biol., April 15, 2005; 25(8): 2957 - 2968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhou, J. G. Krueger, M.-C. J. Kao, E. Lee, F. Du, A. Menter, W. H. Wong, and A. M. Bowcock Novel mechanisms of T-cell and dendritic cell activation revealed by profiling of psoriasis on the 63,100-element oligonucleotide array Physiol Genomics, March 18, 2003; 13(1): 69 - 78. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-J. Jung, J. S. Isaacs, S. Lee, J. Trepel, and L. Neckers Microtubule Disruption Utilizes an NFkappa B-dependent Pathway to Stabilize HIF-1alpha Protein J. Biol. Chem., February 21, 2003; 278(9): 7445 - 7452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Carre, N. Andre, G. Carles, H. Borghi, L. Brichese, C. Briand, and D. Braguer Tubulin Is an Inherent Component of Mitochondrial Membranes That Interacts with the Voltage-dependent Anion Channel J. Biol. Chem., September 6, 2002; 277(37): 33664 - 33669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. G. Mackenzie, M. P. Zago, C. L. Keen, and P. I. Oteiza Low Intracellular Zinc Impairs the Translocation of Activated NF-kappa B to the Nuclei in Human Neuroblastoma IMR-32 Cells J. Biol. Chem., September 6, 2002; 277(37): 34610 - 34617. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
