Molecular Pharmacology, Vol 6, 184-188, Copyright © 1970 by the American Society for Pharmacology and Experimental Therapeutics

Cyclic Adenosine 3',5'-Monophosphate Formation in Brain Slices: Stimulation by Batrachotoxin, Ouabain, Veratridine, and Potassium Ions

¹ National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014

The formation of cyclic adenosine 3',5'-monophosphate-¹⁴C from endogenous ATP-¹⁴C is greatly stimulated on incubation of cerebral cortex slices with depolarizing agents such as batrachotoxin, veratridine, ouabain, and potassium ion. In slices of cerebral cortex, batrachotoxin is the most potent known stimulant (ED₅₀ = 1 x 10^-7 M) of cyclic 3',5'-AMP-¹⁴C formation. Stimulation of cyclic 3',5'-AMP-¹⁴C formation by depolarizing agents requires calcium ions and is inhibited by theophylline. Stimulation of cyclic 3',5'-AMP-¹⁴C formation by histamine, in contrast, does not require calcium ions and is not inhibited by theophylline. The results suggest a relationship among depolarization, transmitter release, and cyclic 3',5'-AMP formation in brain.

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