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Molecular Pharmacology, Vol 6, 255-265, Copyright © 1970 by the American Society for Pharmacology and Experimental Therapeutics

The Interaction of the Nucleoside Analogues, Formycins A and B, with Xanthine Oxidase and Hepatic Aldehyde Oxidase

M. R. SHEEN 1, H. F. MARTIN 1, and R. E. PARKS JR. 1

1 Division of Biological and Medical Sciences, Brown University, Providence, Rhode Island 02912

Formycin B, an analogue of inosine, has been found to be oxidized to oxoformycin B, the xanthosine analogue, by hepatic aldehyde oxidase (EC 1.2.3.1). The Km was 2.0 x 10-4 M. Formycin B and formycin A, the adenosine analogue, are not substrates but are competitive inhibitors for the related enzyme, xanthine oxidase, with Ki values of 1.3 x 10-5 M. Neither of these enzymes has previously been known to interact with purine nucleosides or close structural analogues of nucleosides. The effects of several related purine base and nucleoside analogues on hepatic aldehyde oxidase, bovine milk xanthine oxidase (EC 1.2.3.2), calf intestinal adenosine deaminase (EC 3.5.4.4), and human erythrocytic purine nucleoside phosphorylase (EC (2.4.2.1) are reported. A method for purifying aldehyde oxidase from rabbit liver is described.

Submitted on November 3, 1969






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