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Molecular Pharmacology, Vol 6, 441-443, Copyright © 1970 by the American Society for Pharmacology and Experimental Therapeutics

The Binding of Tritiated Ouabain to Sodium- and Potassium-Activated Adenosine Triphosphatase and Cardiac Relaxing System of Perfused Dog Heart

JULIUS C. ALLEN 1, HENRY R. BESCH JR. 1, GERALD GLICK 1, ARNOLD SCHWARTZ 1, and ROBERT M. LEWIS 1

1 Division of Myocardial Biology and Laboratory of Clinical Cardiovascular Pharmacology. Baylor College of Medicine, Houston, Texas 77025

Isolated dog hearts were perfused in situ with ouabain; control hearts were perfused for similar lengths of time without the addition of ouabain. After the onset of a significant positive inotropic effect, the hearts were removed, and (Na+ + K+)-ATPase and cardiac relaxing system were isolated. The (Na+ + K+)-ATPase activity was significantly decreased in the ouabain-treated hearts. The binding in vitro of 3H-ouabain to the (Na+ + K+)-ATPase of the treated hearts was significantly lower than biniding to control enzyme. A small extent of binding of 3H-ouabain to the cardiac relaxing system was observed and may be attributed to the presence of (Na+ + K+)-ATPase.

Submitted on January 30, 1970






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