![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 60, Issue 5, 989-998, November 2001
Departments of Neuropharmacology (H.K., M.K., S.K.), Pharmacology
(A.A.), and Molecular Pharmacology (M.S.), Graduate School of
Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan; and
Center for Integrative Bioscience (Y.M.), Okazaki National Research
Institutes, Okazaki, Japan
We studied the opening mechanism of Ca2+-permeable channels
formed with mouse transient receptor potential type 5 (mTRP5) using Xenopus oocytes. After stimulation of coexpressed
muscarinic M1 receptors with acetylcholine (ACh) in a
Ca2+-free solution, switching to 2 mM
Ca2+-containing solution evoked a large Cl
current, which reflects the opening of endogenous
Ca2+-dependent Cl channels following
Ca2+ entry through the expressed channels. The ACh-evoked
response was not affected by a depletion of Ca2+ store with
thapsigargin but was inhibited by preinjection of antisense
oligodeoxynucleotides (ODNs) to Gq, G11, or
both. The mTRP5 channel response was also induced by a direct
activation of G proteins with injection of guanosine
5'-3-O-(thio)triphosphate (GTP
S). The ACh- and
GTPS-evoked responses were inhibited by either pretreatment with a
phospholipase C inhibitor, U73122, or an inositol-1,4,5-trisphosphate
(IP3) receptor inhibitor, xestospongin C (XeC). An
activation of IP3 receptors with injection of adenophostin A (AdA) evoked the mTRP5 channel response in a dose-dependent manner.
The AdA-evoked response was not suppressed by preinjection of antisense
ODNs to Gq/11 or U73122 but was suppressed by either
preinjection of XeC or a peptide mimicking the IP3 binding domain of Xenopus IP3 receptor. These
findings suggest that the activation of IP3 receptor is
essential for the opening of mTRP5 channels, and that neither G
proteins, phosphoinositide metabolism, nor depletion of the
Ca2+ store directly modifies the IP3
receptor-linked opening of mTRP5 channels.
This article has been cited by other articles:
|
|
 |
|
  J. Abramowitz and L. Birnbaumer Physiology and pathophysiology of canonical transient receptor potential channels FASEB J, February 1, 2009; 23(2): 297 - 328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Brann and D. A. Fadool Vomeronasal sensory neurons from Sternotherus odoratus (stinkpot/musk turtle) respond to chemosignals via the phospholipase C system J. Exp. Biol., May 15, 2006; 209(10): 1914 - 1927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Shimizu, T. Yoshida, M. Wakamori, M. Ishii, T. Okada, M. Takahashi, M. Seto, K. Sakurada, Y. Kiuchi, and Y. Mori Ca2+-calmodulin-dependent myosin light chain kinase is essential for activation of TRPC5 channels expressed in HEK293 cells J. Physiol., January 15, 2006; 570(2): 219 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. N. Chernova, D. H. Vandorpe, J. S. Clark, J. I. Williams, M. A. Zasloff, L. Jiang, and S. L. Alper Apparent receptor-mediated activation of Ca2+-dependent conductive Cl- transport by shark-derived polyaminosterols Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2005; 289(6): R1644 - R1658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Ordaz, J. Tang, R. Xiao, A. Salgado, A. Sampieri, M. X. Zhu, and L. Vaca Calmodulin and Calcium Interplay in the Modulation of TRPC5 Channel Activity: IDENTIFICATION OF A NOVEL C-TERMINAL DOMAIN FOR CALCIUM/CALMODULIN-MEDIATED FACILITATION J. Biol. Chem., September 2, 2005; 280(35): 30788 - 30796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. H. Zhu, M. Chae, H. J. Kim, Y. M. Lee, M. J. Kim, N. G. Jin, D. K. Yang, I. So, and K. W. Kim Desensitization of canonical transient receptor potential channel 5 by protein kinase C Am J Physiol Cell Physiol, September 1, 2005; 289(3): C591 - C600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Zeng, S.-Z. Xu, P. K. Jackson, D. McHugh, B. Kumar, S. J. Fountain, and D. J. Beech Human TRPC5 channel activated by a multiplicity of signals in a single cell J. Physiol., September 15, 2004; 559(3): 739 - 750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Weiss, T. Doan, K. E. Bernstein, and N. Dascal Modulation of Cardiac Ca2+ Channel by Gq-activating Neurotransmitters Reconstituted in Xenopus Oocytes J. Biol. Chem., March 26, 2004; 279(13): 12503 - 12510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Venkatachalam, F. Zheng, and D. L. Gill Regulation of Canonical Transient Receptor Potential (TRPC) Channel Function by Diacylglycerol and Protein Kinase C J. Biol. Chem., August 1, 2003; 278(31): 29031 - 29040. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kawanabe, Y. Okamoto, S. Miwa, N. Hashimoto, and T. Masaki Molecular Mechanisms for the Activation of Voltage-Independent Ca2+ Channels by Endothelin-1 in Chinese Hamster Ovary Cells Stably Expressing Human EndothelinA Receptors Mol. Pharmacol., July 1, 2002; 62(1): 75 - 80. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
