![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 60, Issue 6, 1288-1295, December 2001
Department of Cell and Cancer Biology, National Cancer Institute,
Bethesda, Maryland (M.S.J., J.B.T.); and Department of Biochemistry,
Vanderbilt University School of Medicine, Nashville, Tennessee (C.W.)
Methylation of DNA is associated with gene silencing. DNA methylation
uses S-adenosylmethionine (SAM) as the methyl donor and
the formation of SAM requires a continuous supply of folate from the
extracellular milieu. Low extracellular folate levels are known to
result in induction of expression of the human 
folate receptor in
nasopharyngeal epidermoid carcinoma cells. Low folate levels have been
implicated in global activation of gene expression. We have
investigated the impact of lowering the level of extracellular folate
by performing cDNA microarray analysis of global gene expression in
human nasopharyngeal carcinoma KB cells grown in folate-deplete and
folate-replete medium. We found that expression of only eight genes
reproducibly responded to variation of folate levels. Among those,
three were up-regulated and five were down-regulated. Examination of
one gene, H-cadherin, demonstrated down-regulation in response
to folate depletion. Despite the low level of extracellular folate,
there was hypermethylation of H-cadherin 5' sequences. These data
indicate that low extracellular folate positively and negatively
influences the expression levels of a small cohort of genes. The data
suggest that folate deficiency is associated with gene-specific
methylation/demethylation, rather than global DNA demethylation and
transcriptional activation.
This article has been cited by other articles:
|
|
 |
|
  J. Kotsopoulos, K.-J. Sohn, and Y.-I. Kim Postweaning Dietary Folate Deficiency Provided through Childhood to Puberty Permanently Increases Genomic DNA Methylation in Adult Rat Liver J. Nutr., April 1, 2008; 138(4): 703 - 709. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A D. Smith, Y.-I. Kim, and H. Refsum Is folic acid good for everyone? Am. J. Clinical Nutrition, March 1, 2008; 87(3): 517 - 533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Sadikovic, J. Andrews, D. Carter, J. Robinson, and D. I. Rodenhiser Genome-wide H3K9 Histone Acetylation Profiles Are Altered in Benzopyrene-treated MCF7 Breast Cancer Cells J. Biol. Chem., February 15, 2008; 283(7): 4051 - 4060. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D. Akintola, Z. L. Crislip, J. M. Catania, G. Chen, W. E. Zimmer, R. C. Burghardt, and A. R. Parrish Promoter methylation is associated with the age-dependent loss of N-cadherin in the rat kidney Am J Physiol Renal Physiol, January 1, 2008; 294(1): F170 - F176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. S. Dhillon, M. Shahid, and S. A. Husain Associations of MTHFR DNMT3b 4977 bp deletion in mtDNA and GSTM1 deletion, and aberrant CpG island hypermethylation of GSTM1 in non-obstructive infertility in Indian men Mol. Hum. Reprod., April 1, 2007; 13(4): 213 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Hayashi, K.-J. Sohn, J. M. Stempak, R. Croxford, and Y.-I. Kim Folate Deficiency Induces Cell-Specific Changes in the Steady-State Transcript Levels of Genes Involved in Folate Metabolism and 1-Carbon Transfer Reactions in Human Colonic Epithelial Cells J. Nutr., March 1, 2007; 137(3): 607 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. K. W. To, Z. Zhan, and S. E. Bates Aberrant Promoter Methylation of the ABCG2 Gene in Renal Carcinoma Mol. Cell. Biol., November 15, 2006; 26(22): 8572 - 8585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Wasson, A. P. McGlynn, H. McNulty, S. L. O'Reilly, V. J. McKelvey-Martin, G. McKerr, J. J. Strain, J. Scott, and C. S. Downes Global DNA and p53 Region-Specific Hypomethylation in Human Colonic Cells Is Induced by Folate Depletion and Reversed by Folate Supplementation J. Nutr., November 1, 2006; 136(11): 2748 - 2753. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Leopardi, F. Marcon, S. Caiola, A. Cafolla, E. Siniscalchi, A. Zijno, and R. Crebelli Effects of folic acid deficiency and MTHFR C677T polymorphism on spontaneous and radiation-induced micronuclei in human lymphocytes Mutagenesis, September 1, 2006; 21(5): 327 - 333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Anguera, M. S. Field, C. Perry, H. Ghandour, E.-P. Chiang, J. Selhub, B. Shane, and P. J. Stover Regulation of Folate-mediated One-carbon Metabolism by 10-Formyltetrahydrofolate Dehydrogenase J. Biol. Chem., July 7, 2006; 281(27): 18335 - 18342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Novakovic, J. M. Stempak, K.-J. Sohn, and Y.-I. Kim Effects of folate deficiency on gene expression in the apoptosis and cancer pathways in colon cancer cells Carcinogenesis, May 1, 2006; 27(5): 916 - 924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Jang, J. B. Mason, and S.-W. Choi Genetic and Epigenetic Interactions between Folate and Aging in Carcinogenesis J. Nutr., December 1, 2005; 135(12): 2967S - 2971S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-I. Kim Nutritional Epigenetics: Impact of Folate Deficiency on DNA Methylation and Colon Cancer Susceptibility J. Nutr., November 1, 2005; 135(11): 2703 - 2709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Stempak, K.-J. Sohn, E.-P. Chiang, B. Shane, and Y.-I. Kim Cell and stage of transformation-specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model Carcinogenesis, May 1, 2005; 26(5): 981 - 990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Liu, Y. Ge, D. C. Cabelof, A. Aboukameel, A. R. Heydari, R. Mohammad, and L. H. Matherly Structure and Regulation of the Murine Reduced Folate Carrier Gene: IDENTIFICATION OF FOUR NONCODING EXONS AND PROMOTERS AND REGULATION BY DIETARY FOLATES J. Biol. Chem., February 18, 2005; 280(7): 5588 - 5597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W.L. Ma, R. H. Finnell, L. A. Davidson, E. S. Callaway, O. Spiegelstein, J. A. Piedrahita, J. M. Salbaum, C. Kappen, B. R. Weeks, J. James, et al. Folate Transport Gene Inactivation in Mice Increases Sensitivity to Colon Carcinogenesis Cancer Res., February 1, 2005; 65(3): 887 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Davis and E. O. Uthus DNA Methylation, Cancer Susceptibility, and Nutrient Interactions Experimental Biology and Medicine, November 1, 2004; 229(10): 988 - 995. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Courtemanche, I. Elson-Schwab, S. T. Mashiyama, N. Kerry, and B. N. Ames Folate Deficiency Inhibits the Proliferation of Primary Human CD8+ T Lymphocytes In Vitro J. Immunol., September 1, 2004; 173(5): 3186 - 3192. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-I. Kim Folate and DNA Methylation: A Mechanistic Link between Folate Deficiency and Colorectal Cancer? Cancer Epidemiol. Biomarkers Prev., April 1, 2004; 13(4): 511 - 519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Crott, S.-W. Choi, J. M. Ordovas, J. S. Ditelberg, and J. B. Mason Effects of dietary folate and aging on gene expression in the colonic mucosa of rats: implications for carcinogenesis Carcinogenesis, January 1, 2004; 25(1): 69 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. V. Oleinik and S. A. Krupenko Ectopic Expression of 10-Formyltetrahydrofolate Dehydrogenase in A549 Cells Induces G1 Cell Cycle Arrest and Apoptosis Mol. Cancer Res., June 1, 2003; 1(8): 577 - 588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-J. Sohn, J. M. Stempak, S. Reid, S. Shirwadkar, J. B. Mason, and Y.-I. Kim The effect of dietary folate on genomic and p53-specific DNA methylation in rat colon Carcinogenesis, January 1, 2003; 24(1): 81 - 90. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Friso and S.-W. Choi Gene-Nutrient Interactions and DNA Methylation J. Nutr., August 1, 2002; 132(8): 2382S - 2387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-W. Choi and J. B. Mason Folate Status: Effects on Pathways of Colorectal Carcinogenesis J. Nutr., August 1, 2002; 132(8): 2413S - 2418. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
