MolPharm xPharm- The Comprehensive Pharmacology Reference

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Börner, C.
Right arrow Articles by Kraus, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Börner, C.
Right arrow Articles by Kraus, J.

Vol. 61, Issue 4, 800-805, April 2002

Involvement of Activator Protein-1 in Transcriptional Regulation of the Human µ-Opioid Receptor Gene

Christine Börner, Volker Höllt, and Jürgen Kraus

Department of Pharmacology, University of Magdeburg, Magdeburg, Germany

µ-Opioid receptors mediate such opioid effects as analgesia, euphoria, and immunomodulation. Gene expression of µ-opioid receptors can be modulated by various substances, including cytokines, hormones, and drugs. Some of these stimuli (e.g., IL-1beta and cocaine) have been shown to activate members of the AP-1 transcription factor family. In addition, transcription of the µ-opioid receptor gene is induced by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, which in turn is an activator of AP-1 transcription factors. This indicates that signaling pathways involving protein kinase C and activator protein 1 (AP-1) transcription factors are important for the specific expression pattern of the µ-opioid receptor gene. In this report, we show that TPA activates AP-1 as well as the transcription factor nuclear factor kappa B (NFkappa B) in the µ-opioid receptor expressing neuroblastoma cell line SH SY5Y. In transfection experiments performed in these cells, both factors trans-activate expression of reporter gene constructs containing the human µ-opioid receptor gene promoter. By excluding the effects of TPA on NFkappa B with the specific NFkappa B inhibitor sulfasalazine, AP-1 regulatory elements were localized. Two AP-1 elements, which differ in one nucleotide each from the classic AP-1 binding site, were delineated to positions -2388 and -1434 of the promoter. Independent of their orientation, these elements conferred TPA responsiveness on the heterologous thymidine kinase promoter. AP-1 binding to these elements was confirmed using electrophoretic mobility shift and immunoshift assays.

Copyright © 2002 by The American Society for Pharmacology and Experimental Therapeutics


This article has been cited by other articles:


Home page
 Mol. Pharmacol.Home page
C. Borner, J. Kraus, A. Bedini, B. Schraven, and V. Hollt
T-Cell Receptor/CD28-Mediated Activation of Human T Lymphocytes Induces Expression of Functional {micro}-Opioid Receptors
Mol. Pharmacol., August 1, 2008; 74(2): 496 - 504.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
D. Chakass, D. Philippe, E. Erdual, S. Dharancy, M. Malapel, C. Dubuquoy, X. Thuru, J. Gay, C. Gaveriaux-Ruff, P. Dubus, et al.
{micro}-Opioid receptor activation prevents acute hepatic inflammation and cell death
Gut, July 1, 2007; 56(7): 974 - 981.
[Abstract] [Full Text] [PDF]

Home page
 The OncologistHome page
J. R. Ross, J. Riley, C. Quigley, and K. I. Welsh
Clinical Pharmacology and Pharmacotherapy of Opioid Switching in Cancer Patients
Oncologist, July 1, 2006; 11(7): 765 - 773.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
D Philippe, D Chakass, X Thuru, P Zerbib, A Tsicopoulos, K Geboes, P Bulois, M Breisse, H Vorng, J Gay, et al.
Mu opioid receptor expression is increased in inflammatory bowel diseases: implications for homeostatic intestinal inflammation
Gut, June 1, 2006; 55(6): 815 - 823.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
C. Borner, V. Hollt, and J. Kraus
Cannabinoid Receptor Type 2 Agonists Induce Transcription of the {micro}-Opioid Receptor Gene in Jurkat T Cells
Mol. Pharmacol., April 1, 2006; 69(4): 1486 - 1491.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
J. Frassdorf, N. C. Weber, D. Obal, O. Toma, J. Mullenheim, G. Kojda, B. Preckel, and W. Schlack
Morphine Induces Late Cardioprotection in Rat Hearts In Vivo: The Involvement of Opioid Receptors and Nuclear Transcription Factor {kappa}B
Anesth. Analg., October 1, 2005; 101(4): 934 - 941.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
C. Borner, J. Kraus, H. Schroder, H. Ammer, and V. Hollt
Transcriptional Regulation of the Human {micro}-Opioid Receptor Gene by Interleukin-6
Mol. Pharmacol., December 1, 2004; 66(6): 1719 - 1726.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
J. Kraus, C. Borner, E. Giannini, and V. Hollt
The Role of Nuclear Factor {kappa}B in Tumor Necrosis Factor-Regulated Transcription of the Human {micro}-Opioid Receptor Gene
Mol. Pharmacol., October 1, 2003; 64(4): 876 - 884.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 2002 by the American Society for Pharmacology and Experimental Therapeutics